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Characterization of an Unknown Region Linked to the Glycoside Hydrolase Family 17 β-1,3-Glucanase of Vibrio vulnificus Reveals a Novel Glucan-Binding Domain
The glycoside hydrolase family 17 β-1,3-glucanase of Vibrio vulnificus (VvGH17) has two unknown regions in the N- and C-termini. Here, we characterized these domains by preparing mutant enzymes. VvGH17 demonstrated hydrolytic activity of β-(1→3)-glucan, mainly producing laminaribiose, but not of β-(...
Autores principales: | Kumagai, Yuya, Kishimura, Hideki, Lang, Weeranuch, Tagami, Takayoshi, Okuyama, Masayuki, Kimura, Atsuo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026390/ https://www.ncbi.nlm.nih.gov/pubmed/35447923 http://dx.doi.org/10.3390/md20040250 |
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