Effects of Parity and Stage of Lactation on Trend and Variability of Metabolic Markers in Dairy Cows

SIMPLE SUMMARY: The rise in milk yield per cow, herd size, and the percentage of primiparous cows in dairy herds increasingly requires optimized health management in order to ensure the health of the cows. The transition period (three weeks before to three weeks after calving) has a key role in health problems, because dairy cows undergo tremendous metabolic changes. Metabolic monitoring provides an in-depth insight into how the cows cope with these challenges. A remarkable variability in the metabolic parameters reflects the adaptation of dairy cows during the transition from pregnancy to lactation. In addition, primiparous cows undergo physical adaptations because of growth, first gestation, the maturation of the mammary glands, the onset of lactation, and fighting for social dominance. Previous studies have rarely considered these specific demands due to the influences of parity and the lactation stage. Thus, the objective of our study was to describe the variation in metabolic parameters due to parity and the stage of lactation based on a huge number of primiparous and multiparous cows, observed at all stages of lactation, in a retrospective analysis of laboratory data. The remarkable impact of both parity and lactation was elucidated for most parameters. This should be taken into account for a correct interpretation of the laboratory diagnostics in the framework of metabolic monitoring. ABSTRACT: Metabolic monitoring is a tool that is helpful with the increasing requirements regarding feeding and health management in dairy herds. This study aimed at describing the trend and variability of different biochemical parameters in blood and urine in relation to the stages of lactation and parity, in a retrospective analysis of laboratory data from clinically healthy German Holstein cows. The results were derived from metabolic monitoring in Thuringia (Germany), during 2009–2019. A total of 361,584 measured values, of 13 different metabolic variables, were assigned to parity (primiparous and multiparous) and stage of lactation (10 classes from −30 to 300 days in milk). The Kruskal–Wallis test was applied for the evaluation of differences regarding parity or the stage of lactation. Non-esterified fatty acids, beta hydroxybutyrate, and the activity of aspartate aminotransferase in serum were clearly affected by parity and lactation. Serum concentrations of cholesterol, bilirubin, and phosphorus, as well as the serum activity of glutamate dehydrogenase, were affected by the stage of lactation, while parity impacted urea concentration. The serum activity of creatine kinase, serum concentrations of calcium, and urine concentrations of net acid base excretion, potassium, and sodium were not affected by parity or lactation. In conclusion, specific reference limits, with respect to parity and the stage of lactation, are necessary.