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Putrescine Production by Latilactobacillus curvatus KP 3-4 Isolated from Fermented Foods

Polyamines are aliphatic hydrocarbons with terminal amino groups and are essential for biological activities. It has been reported that polyamines have health-promoting effects in animals, such as the extension of lifespan by polyamine intake. The identification of a high polyamine-producing bacteri...

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Detalles Bibliográficos
Autores principales: Hirano, Rika, Kume, Aiko, Nishiyama, Chisato, Honda, Ryosuke, Shirasawa, Hideto, Ling, Yiwei, Sugiyama, Yuta, Nara, Misaki, Shimokawa, Hiromi, Kawada, Hiroki, Koyanagi, Takashi, Ashida, Hisashi, Okuda, Shujiro, Matsumoto, Mitsuharu, Takagi, Hiroki, Kurihara, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026525/
https://www.ncbi.nlm.nih.gov/pubmed/35456748
http://dx.doi.org/10.3390/microorganisms10040697
Descripción
Sumario:Polyamines are aliphatic hydrocarbons with terminal amino groups and are essential for biological activities. It has been reported that polyamines have health-promoting effects in animals, such as the extension of lifespan by polyamine intake. The identification of a high polyamine-producing bacterium from foods could lead to the development of a novel probiotic candidate. We aimed to identify high polyamine-producing bacteria from food, and isolated and collected bacteria from vegetables and fermented foods produced in Japan. We successfully acquired Latilactobacillus curvatus KP 3-4 isolated from Kabura-zushi as a putrescine producing lactic acid bacteria. Comparing the polyamine synthesis capability of L. curvatus KP 3-4 with that of typical probiotic lactic acid bacteria and L. curvatus strains available from the Japan Collection of Microorganisms, it was found that only L. curvatus KP 3-4 was capable of exporting high levels of putrescine into the culture supernatant. The enhancement of putrescine production by the addition of ornithine, and whole-genome analysis of L. curvatus KP 3-4, suggest that putrescine is synthesized via ornithine decarboxylase. The administration of L. curvatus KP 3-4 to germ-free mice increased the concentration of putrescine in the feces.