Cargando…

An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation

An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe(3)O(4) magnetic nanoparticles were conjugated with protein G for imm...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Jie-Biao, Cheng, Jin-Sheng, Wei, Tai-Long, Wu, Fan-Min, Tang, Gui-Hong, He, Qing-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026555/
https://www.ncbi.nlm.nih.gov/pubmed/35454740
http://dx.doi.org/10.3390/foods11081155
_version_ 1784691150924283904
author Guo, Jie-Biao
Cheng, Jin-Sheng
Wei, Tai-Long
Wu, Fan-Min
Tang, Gui-Hong
He, Qing-Hua
author_facet Guo, Jie-Biao
Cheng, Jin-Sheng
Wei, Tai-Long
Wu, Fan-Min
Tang, Gui-Hong
He, Qing-Hua
author_sort Guo, Jie-Biao
collection PubMed
description An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe(3)O(4) magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents. The immuno-separation of fluorescent reporters by magnetic adsorbents could be completed by ochratoxin A, so the fluorescent reporters released from the immune complex indicate a linear correlation with the concentration of ochratoxin A. Furthermore, the immuno-separated ochratoxin A can be eluted from magnetic adsorbent for LC-conformation. The optimized assay showed results as follows: the quantitative range of the immuno-separated assay was 0.03–100 ng mL(−1) of ochratoxin A. The recoveries for spiked samples ranged from 78.2% to 91.4%, with the relative standard deviation (RSD) being 11.9%~15.3%. Statistical analysis indicated no significant difference between the HPLC-FLD results based on commercial affinity column and by nano-affinity cleaning up.
format Online
Article
Text
id pubmed-9026555
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90265552022-04-23 An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation Guo, Jie-Biao Cheng, Jin-Sheng Wei, Tai-Long Wu, Fan-Min Tang, Gui-Hong He, Qing-Hua Foods Article An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe(3)O(4) magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents. The immuno-separation of fluorescent reporters by magnetic adsorbents could be completed by ochratoxin A, so the fluorescent reporters released from the immune complex indicate a linear correlation with the concentration of ochratoxin A. Furthermore, the immuno-separated ochratoxin A can be eluted from magnetic adsorbent for LC-conformation. The optimized assay showed results as follows: the quantitative range of the immuno-separated assay was 0.03–100 ng mL(−1) of ochratoxin A. The recoveries for spiked samples ranged from 78.2% to 91.4%, with the relative standard deviation (RSD) being 11.9%~15.3%. Statistical analysis indicated no significant difference between the HPLC-FLD results based on commercial affinity column and by nano-affinity cleaning up. MDPI 2022-04-15 /pmc/articles/PMC9026555/ /pubmed/35454740 http://dx.doi.org/10.3390/foods11081155 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Jie-Biao
Cheng, Jin-Sheng
Wei, Tai-Long
Wu, Fan-Min
Tang, Gui-Hong
He, Qing-Hua
An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
title An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
title_full An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
title_fullStr An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
title_full_unstemmed An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
title_short An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
title_sort immuno-separated assay for ochratoxin detection coupled with a nano-affinity cleaning-up for lc-confirmation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026555/
https://www.ncbi.nlm.nih.gov/pubmed/35454740
http://dx.doi.org/10.3390/foods11081155
work_keys_str_mv AT guojiebiao animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT chengjinsheng animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT weitailong animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT wufanmin animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT tangguihong animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT heqinghua animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT guojiebiao immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT chengjinsheng immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT weitailong immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT wufanmin immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT tangguihong immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation
AT heqinghua immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation