Cargando…
An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe(3)O(4) magnetic nanoparticles were conjugated with protein G for imm...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026555/ https://www.ncbi.nlm.nih.gov/pubmed/35454740 http://dx.doi.org/10.3390/foods11081155 |
_version_ | 1784691150924283904 |
---|---|
author | Guo, Jie-Biao Cheng, Jin-Sheng Wei, Tai-Long Wu, Fan-Min Tang, Gui-Hong He, Qing-Hua |
author_facet | Guo, Jie-Biao Cheng, Jin-Sheng Wei, Tai-Long Wu, Fan-Min Tang, Gui-Hong He, Qing-Hua |
author_sort | Guo, Jie-Biao |
collection | PubMed |
description | An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe(3)O(4) magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents. The immuno-separation of fluorescent reporters by magnetic adsorbents could be completed by ochratoxin A, so the fluorescent reporters released from the immune complex indicate a linear correlation with the concentration of ochratoxin A. Furthermore, the immuno-separated ochratoxin A can be eluted from magnetic adsorbent for LC-conformation. The optimized assay showed results as follows: the quantitative range of the immuno-separated assay was 0.03–100 ng mL(−1) of ochratoxin A. The recoveries for spiked samples ranged from 78.2% to 91.4%, with the relative standard deviation (RSD) being 11.9%~15.3%. Statistical analysis indicated no significant difference between the HPLC-FLD results based on commercial affinity column and by nano-affinity cleaning up. |
format | Online Article Text |
id | pubmed-9026555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90265552022-04-23 An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation Guo, Jie-Biao Cheng, Jin-Sheng Wei, Tai-Long Wu, Fan-Min Tang, Gui-Hong He, Qing-Hua Foods Article An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe(3)O(4) magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents. The immuno-separation of fluorescent reporters by magnetic adsorbents could be completed by ochratoxin A, so the fluorescent reporters released from the immune complex indicate a linear correlation with the concentration of ochratoxin A. Furthermore, the immuno-separated ochratoxin A can be eluted from magnetic adsorbent for LC-conformation. The optimized assay showed results as follows: the quantitative range of the immuno-separated assay was 0.03–100 ng mL(−1) of ochratoxin A. The recoveries for spiked samples ranged from 78.2% to 91.4%, with the relative standard deviation (RSD) being 11.9%~15.3%. Statistical analysis indicated no significant difference between the HPLC-FLD results based on commercial affinity column and by nano-affinity cleaning up. MDPI 2022-04-15 /pmc/articles/PMC9026555/ /pubmed/35454740 http://dx.doi.org/10.3390/foods11081155 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guo, Jie-Biao Cheng, Jin-Sheng Wei, Tai-Long Wu, Fan-Min Tang, Gui-Hong He, Qing-Hua An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
title | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
title_full | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
title_fullStr | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
title_full_unstemmed | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
title_short | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
title_sort | immuno-separated assay for ochratoxin detection coupled with a nano-affinity cleaning-up for lc-confirmation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026555/ https://www.ncbi.nlm.nih.gov/pubmed/35454740 http://dx.doi.org/10.3390/foods11081155 |
work_keys_str_mv | AT guojiebiao animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT chengjinsheng animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT weitailong animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT wufanmin animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT tangguihong animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT heqinghua animmunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT guojiebiao immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT chengjinsheng immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT weitailong immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT wufanmin immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT tangguihong immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation AT heqinghua immunoseparatedassayforochratoxindetectioncoupledwithananoaffinitycleaningupforlcconfirmation |