Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)

Infertility is a common disease that impacts 15% of reproductive age couples worldwide, and genetic causes are implicated in about half of those cases. Non-obstructive azoospermia is a severe form of male infertility that features spermatogenic failure resulting in no sperm in the ejaculate and seve...

Descripción completa

Detalles Bibliográficos
Autores principales: Tran, Kien T.D., Sheng, Yi, Doungkamchan, Chatchanan, Castro, Carlo A., Orwig, Kyle E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026566/
https://www.ncbi.nlm.nih.gov/pubmed/35240466
http://dx.doi.org/10.1016/j.scr.2022.102728
_version_ 1784691153598152704
author Tran, Kien T.D.
Sheng, Yi
Doungkamchan, Chatchanan
Castro, Carlo A.
Orwig, Kyle E.
author_facet Tran, Kien T.D.
Sheng, Yi
Doungkamchan, Chatchanan
Castro, Carlo A.
Orwig, Kyle E.
author_sort Tran, Kien T.D.
collection PubMed
description Infertility is a common disease that impacts 15% of reproductive age couples worldwide, and genetic causes are implicated in about half of those cases. Non-obstructive azoospermia is a severe form of male infertility that features spermatogenic failure resulting in no sperm in the ejaculate and severely reduces the chance to have biological children. We created a Tex11_1260Ins(TT) (1260GATA → TTGGTA) mutant mouse that models the Tex11_1258(TT) mutation identified from a patient with nonobstructive azoospermia. The Tex11_1260Ins(TT) iPSC cells displayed characteristics of pluripotent-like morphology, expressed pluripotent protein markers, show normal karyotype, and can to differentiate into tissues of the three germ layers.
format Online
Article
Text
id pubmed-9026566
institution National Center for Biotechnology Information
language English
publishDate 2022
record_format MEDLINE/PubMed
spelling pubmed-90265662022-04-22 Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT) Tran, Kien T.D. Sheng, Yi Doungkamchan, Chatchanan Castro, Carlo A. Orwig, Kyle E. Stem Cell Res Article Infertility is a common disease that impacts 15% of reproductive age couples worldwide, and genetic causes are implicated in about half of those cases. Non-obstructive azoospermia is a severe form of male infertility that features spermatogenic failure resulting in no sperm in the ejaculate and severely reduces the chance to have biological children. We created a Tex11_1260Ins(TT) (1260GATA → TTGGTA) mutant mouse that models the Tex11_1258(TT) mutation identified from a patient with nonobstructive azoospermia. The Tex11_1260Ins(TT) iPSC cells displayed characteristics of pluripotent-like morphology, expressed pluripotent protein markers, show normal karyotype, and can to differentiate into tissues of the three germ layers. 2022-04 2022-02-25 /pmc/articles/PMC9026566/ /pubmed/35240466 http://dx.doi.org/10.1016/j.scr.2022.102728 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Tran, Kien T.D.
Sheng, Yi
Doungkamchan, Chatchanan
Castro, Carlo A.
Orwig, Kyle E.
Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)
title Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)
title_full Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)
title_fullStr Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)
title_full_unstemmed Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)
title_short Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11_1260Ins(TT)
title_sort induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation tex11_1260ins(tt)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026566/
https://www.ncbi.nlm.nih.gov/pubmed/35240466
http://dx.doi.org/10.1016/j.scr.2022.102728
work_keys_str_mv AT trankientd inducedpluripotentstemcelllinefromamousemodelofhumanazoospermiawithaframeshiftmutationtex111260instt
AT shengyi inducedpluripotentstemcelllinefromamousemodelofhumanazoospermiawithaframeshiftmutationtex111260instt
AT doungkamchanchatchanan inducedpluripotentstemcelllinefromamousemodelofhumanazoospermiawithaframeshiftmutationtex111260instt
AT castrocarloa inducedpluripotentstemcelllinefromamousemodelofhumanazoospermiawithaframeshiftmutationtex111260instt
AT orwigkylee inducedpluripotentstemcelllinefromamousemodelofhumanazoospermiawithaframeshiftmutationtex111260instt

Ejemplares similares