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DNA replication profiling by molecular combing on single DNA fibers

In this protocol, the progression of DNA synthesis is profiled at a single-molecule resolution. DNA fibers are uniformly stretched on silanized coverslips, and replicating DNA can be traced with thymidine analogs using specific antibodies against distinct analogs. Single DNA fibers are visualized by...

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Detalles Bibliográficos
Autores principales:	Fu, Haiqing, Aladjem, Mirit I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026583/ https://www.ncbi.nlm.nih.gov/pubmed/35463463 http://dx.doi.org/10.1016/j.xpro.2022.101290

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