A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation

Glia are important for the function of touch receptors. Here, we present a protocol in Caenorhabditis elegans for calcium and chloride imaging in worm glia upon nose touch stimulation. We describe aspects of the procedure that are essential for data reproducibility, including worm immobilization, po...

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Detalles Bibliográficos
Autores principales: Fernandez-Abascal, Jesus, Bianchi, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026654/
https://www.ncbi.nlm.nih.gov/pubmed/35463465
http://dx.doi.org/10.1016/j.xpro.2022.101282
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author Fernandez-Abascal, Jesus
Bianchi, Laura
author_facet Fernandez-Abascal, Jesus
Bianchi, Laura
author_sort Fernandez-Abascal, Jesus
collection PubMed
description Glia are important for the function of touch receptors. Here, we present a protocol in Caenorhabditis elegans for calcium and chloride imaging in worm glia upon nose touch stimulation. We describe aspects of the procedure that are essential for data reproducibility, including worm immobilization, poking angle, and applied force. We then detail data processing and analysis of calcium and chloride transients in glia. This protocol can be used for other types of mechanical stimulations or for stimulation using odorants. For complete details on the use and execution of this protocol, please refer to Fernandez-Abascal et al., 2022.
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spelling pubmed-90266542022-04-23 A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation Fernandez-Abascal, Jesus Bianchi, Laura STAR Protoc Protocol Glia are important for the function of touch receptors. Here, we present a protocol in Caenorhabditis elegans for calcium and chloride imaging in worm glia upon nose touch stimulation. We describe aspects of the procedure that are essential for data reproducibility, including worm immobilization, poking angle, and applied force. We then detail data processing and analysis of calcium and chloride transients in glia. This protocol can be used for other types of mechanical stimulations or for stimulation using odorants. For complete details on the use and execution of this protocol, please refer to Fernandez-Abascal et al., 2022. Elsevier 2022-04-13 /pmc/articles/PMC9026654/ /pubmed/35463465 http://dx.doi.org/10.1016/j.xpro.2022.101282 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Fernandez-Abascal, Jesus
Bianchi, Laura
A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation
title A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation
title_full A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation
title_fullStr A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation
title_full_unstemmed A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation
title_short A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation
title_sort protocol for imaging calcium and chloride in c. elegans glia upon touch stimulation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026654/
https://www.ncbi.nlm.nih.gov/pubmed/35463465
http://dx.doi.org/10.1016/j.xpro.2022.101282
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