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Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability

The dual-sugar intestinal permeability test is a commonly used test to assess changes in gut barrier function. However, it does not identify functional changes and the exact mechanism of damage caused by the increased intestinal permeability. This study aims to explore the application of untargeted...

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Autores principales: Shah, Rohan M., Jadhav, Snehal R., Phan, Laura, Tremellen, Kelton, Tran, Cuong D., Beale, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026773/
https://www.ncbi.nlm.nih.gov/pubmed/35448488
http://dx.doi.org/10.3390/metabo12040302
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author Shah, Rohan M.
Jadhav, Snehal R.
Phan, Laura
Tremellen, Kelton
Tran, Cuong D.
Beale, David J.
author_facet Shah, Rohan M.
Jadhav, Snehal R.
Phan, Laura
Tremellen, Kelton
Tran, Cuong D.
Beale, David J.
author_sort Shah, Rohan M.
collection PubMed
description The dual-sugar intestinal permeability test is a commonly used test to assess changes in gut barrier function. However, it does not identify functional changes and the exact mechanism of damage caused by the increased intestinal permeability. This study aims to explore the application of untargeted metabolomics and lipidomics to identify markers of increased intestinal permeability. Fifty fasting male participants (18–50 years) attended a single visit to conduct the following procedures: assessment of anthropometric measures, assessment of gastrointestinal symptoms, intestinal permeability test, and assessment of blood samples 90 min post-administration of the intestinal permeability test. Rhamnose and lactulose were analysed using gas chromatography-mass spectrometry (GC-MS). Untargeted polar metabolites and lipidomics were assessed by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF MS). There was an elevated lactulose/rhamnose ratio in 27 subjects, indicating increased permeability compared to the remaining 23 control subjects. There were no significant differences between groups in characteristics such as age, body mass index (BMI), weight, height, and waist conference. Fourteen metabolites from the targeted metabolomics data were identified as statistically significant in the plasma samples from intestinal permeability subjects. The untargeted metabolomics and lipidomics analyses yielded fifteen and fifty-one statistically significant features, respectively. Individuals with slightly elevated intestinal permeability had altered energy, nucleotide, and amino acid metabolism, in addition to increased glutamine levels. Whether these biomarkers may be used to predict the early onset of leaky gut warrants further investigation.
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spelling pubmed-90267732022-04-23 Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability Shah, Rohan M. Jadhav, Snehal R. Phan, Laura Tremellen, Kelton Tran, Cuong D. Beale, David J. Metabolites Article The dual-sugar intestinal permeability test is a commonly used test to assess changes in gut barrier function. However, it does not identify functional changes and the exact mechanism of damage caused by the increased intestinal permeability. This study aims to explore the application of untargeted metabolomics and lipidomics to identify markers of increased intestinal permeability. Fifty fasting male participants (18–50 years) attended a single visit to conduct the following procedures: assessment of anthropometric measures, assessment of gastrointestinal symptoms, intestinal permeability test, and assessment of blood samples 90 min post-administration of the intestinal permeability test. Rhamnose and lactulose were analysed using gas chromatography-mass spectrometry (GC-MS). Untargeted polar metabolites and lipidomics were assessed by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF MS). There was an elevated lactulose/rhamnose ratio in 27 subjects, indicating increased permeability compared to the remaining 23 control subjects. There were no significant differences between groups in characteristics such as age, body mass index (BMI), weight, height, and waist conference. Fourteen metabolites from the targeted metabolomics data were identified as statistically significant in the plasma samples from intestinal permeability subjects. The untargeted metabolomics and lipidomics analyses yielded fifteen and fifty-one statistically significant features, respectively. Individuals with slightly elevated intestinal permeability had altered energy, nucleotide, and amino acid metabolism, in addition to increased glutamine levels. Whether these biomarkers may be used to predict the early onset of leaky gut warrants further investigation. MDPI 2022-03-29 /pmc/articles/PMC9026773/ /pubmed/35448488 http://dx.doi.org/10.3390/metabo12040302 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shah, Rohan M.
Jadhav, Snehal R.
Phan, Laura
Tremellen, Kelton
Tran, Cuong D.
Beale, David J.
Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability
title Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability
title_full Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability
title_fullStr Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability
title_full_unstemmed Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability
title_short Plasma Metabolic and Lipidomic Fingerprinting of Individuals with Increased Intestinal Permeability
title_sort plasma metabolic and lipidomic fingerprinting of individuals with increased intestinal permeability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026773/
https://www.ncbi.nlm.nih.gov/pubmed/35448488
http://dx.doi.org/10.3390/metabo12040302
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