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Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice

Vaccination is an effective and economically viable means of protection against the influenza virus, but due to rapid viral evolution, modern seasonal vaccines are not effective enough. Next-generation vaccines are designed to provide protection against a wide range of influenza virus strains, inclu...

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Autores principales: Mintaev, Ramil R., Glazkova, Dina V., Orlova, Olga V., Bogoslovskaya, Elena V., Shipulin, German A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026828/
https://www.ncbi.nlm.nih.gov/pubmed/35455283
http://dx.doi.org/10.3390/vaccines10040534
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author Mintaev, Ramil R.
Glazkova, Dina V.
Orlova, Olga V.
Bogoslovskaya, Elena V.
Shipulin, German A.
author_facet Mintaev, Ramil R.
Glazkova, Dina V.
Orlova, Olga V.
Bogoslovskaya, Elena V.
Shipulin, German A.
author_sort Mintaev, Ramil R.
collection PubMed
description Vaccination is an effective and economically viable means of protection against the influenza virus, but due to rapid viral evolution, modern seasonal vaccines are not effective enough. Next-generation vaccines are designed to provide protection against a wide range of influenza virus strains, including pandemic variants. In our work, we made an epitope-based universal vaccine, rMVA-k1-k2, against the influenza virus based on the modified vaccinia Ankara (MVA) vector and using our own algorithms to select epitopes from conserved fragments of the NP, M1 and HA proteins of influenza A and B. We show that double immunization protects mice with a 67% or greater efficiency against viral influenza pneumonia when infected with various strains of the H1N1, H2N2, H3N2 and H5N1 subtypes of influenza A. In animals, the level of protection provided by the rMVA-k1-k2 vaccine was comparable to that provided by the universal M001 and MVA-NP+M1 (Invictus) vaccines, which have shown success in clinical trials, against strains of the H1N1 and H3N2 subtypes.
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spelling pubmed-90268282022-04-23 Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice Mintaev, Ramil R. Glazkova, Dina V. Orlova, Olga V. Bogoslovskaya, Elena V. Shipulin, German A. Vaccines (Basel) Article Vaccination is an effective and economically viable means of protection against the influenza virus, but due to rapid viral evolution, modern seasonal vaccines are not effective enough. Next-generation vaccines are designed to provide protection against a wide range of influenza virus strains, including pandemic variants. In our work, we made an epitope-based universal vaccine, rMVA-k1-k2, against the influenza virus based on the modified vaccinia Ankara (MVA) vector and using our own algorithms to select epitopes from conserved fragments of the NP, M1 and HA proteins of influenza A and B. We show that double immunization protects mice with a 67% or greater efficiency against viral influenza pneumonia when infected with various strains of the H1N1, H2N2, H3N2 and H5N1 subtypes of influenza A. In animals, the level of protection provided by the rMVA-k1-k2 vaccine was comparable to that provided by the universal M001 and MVA-NP+M1 (Invictus) vaccines, which have shown success in clinical trials, against strains of the H1N1 and H3N2 subtypes. MDPI 2022-03-29 /pmc/articles/PMC9026828/ /pubmed/35455283 http://dx.doi.org/10.3390/vaccines10040534 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mintaev, Ramil R.
Glazkova, Dina V.
Orlova, Olga V.
Bogoslovskaya, Elena V.
Shipulin, German A.
Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice
title Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice
title_full Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice
title_fullStr Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice
title_full_unstemmed Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice
title_short Development of a Universal Epitope-Based Influenza Vaccine and Evaluation of Its Effectiveness in Mice
title_sort development of a universal epitope-based influenza vaccine and evaluation of its effectiveness in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026828/
https://www.ncbi.nlm.nih.gov/pubmed/35455283
http://dx.doi.org/10.3390/vaccines10040534
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