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The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida

Edwardsiella piscicida is a pathogenic bacterium, which can infect a number of fish species and cause a disease termed edwardsiellosis, threatening global fish farming with high prevalence and mortality. Thiamine (Vitamin B1), functioning in the form of thiamine pyrophosphate (TPP), is essential for...

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Autores principales: Liu, Xin, Wang, Xinhui, Sun, Boguang, Sun, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026889/
https://www.ncbi.nlm.nih.gov/pubmed/35456139
http://dx.doi.org/10.3390/pathogens11040464
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author Liu, Xin
Wang, Xinhui
Sun, Boguang
Sun, Li
author_facet Liu, Xin
Wang, Xinhui
Sun, Boguang
Sun, Li
author_sort Liu, Xin
collection PubMed
description Edwardsiella piscicida is a pathogenic bacterium, which can infect a number of fish species and cause a disease termed edwardsiellosis, threatening global fish farming with high prevalence and mortality. Thiamine (Vitamin B1), functioning in the form of thiamine pyrophosphate (TPP), is essential for almost all organisms. Bacteria acquire TPP by biosynthesis or by transportation of exogenous thiamine. TPP availability has been associated with bacterial pathogenicity, but the underlying mechanisms remain to be discovered. The role of thiamine in the pathogenicity of E. piscicida is unknown. In this study, we characterized a thiamine transporter (TT) operon in E. piscicida. The deletion of the TT operon resulted in an intracellular TPP lacking situation, which led to attenuated overall pathogenicity, impaired abilities associated with motility and host cell adhesion, as well as decreased expression of certain flagellar and adhesion genes. Moreover, TPP starvation led to intracellular c-di-GMP reduction, and introducing into the TPP-suppressed mutant strain an exogenous diguanylate cyclase for c-di-GMP synthesis restored the virulence loss. Taken together, this work reveals the involvement of thiamine uptake in the virulence regulation of E. piscicida, with c-di-GMP implicated in the process. These finding could be employed to explore potential drug targets against E. piscicida.
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spelling pubmed-90268892022-04-23 The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida Liu, Xin Wang, Xinhui Sun, Boguang Sun, Li Pathogens Article Edwardsiella piscicida is a pathogenic bacterium, which can infect a number of fish species and cause a disease termed edwardsiellosis, threatening global fish farming with high prevalence and mortality. Thiamine (Vitamin B1), functioning in the form of thiamine pyrophosphate (TPP), is essential for almost all organisms. Bacteria acquire TPP by biosynthesis or by transportation of exogenous thiamine. TPP availability has been associated with bacterial pathogenicity, but the underlying mechanisms remain to be discovered. The role of thiamine in the pathogenicity of E. piscicida is unknown. In this study, we characterized a thiamine transporter (TT) operon in E. piscicida. The deletion of the TT operon resulted in an intracellular TPP lacking situation, which led to attenuated overall pathogenicity, impaired abilities associated with motility and host cell adhesion, as well as decreased expression of certain flagellar and adhesion genes. Moreover, TPP starvation led to intracellular c-di-GMP reduction, and introducing into the TPP-suppressed mutant strain an exogenous diguanylate cyclase for c-di-GMP synthesis restored the virulence loss. Taken together, this work reveals the involvement of thiamine uptake in the virulence regulation of E. piscicida, with c-di-GMP implicated in the process. These finding could be employed to explore potential drug targets against E. piscicida. MDPI 2022-04-13 /pmc/articles/PMC9026889/ /pubmed/35456139 http://dx.doi.org/10.3390/pathogens11040464 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xin
Wang, Xinhui
Sun, Boguang
Sun, Li
The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
title The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
title_full The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
title_fullStr The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
title_full_unstemmed The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
title_short The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
title_sort involvement of thiamine uptake in the virulence of edwardsiella piscicida
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026889/
https://www.ncbi.nlm.nih.gov/pubmed/35456139
http://dx.doi.org/10.3390/pathogens11040464
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