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BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking

Neurons are highly polarized cells requiring precise regulation of trafficking and targeting of membrane proteins to generate and maintain different and specialized compartments, such as axons and dendrites. Disruption of the Golgi apparatus (GA) secretory pathway in developing neurons alters axon/d...

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Autores principales: Gastaldi, Laura, Martín, Josefina Inés, Sosa, Lucas Javier, Quassollo, Gonzalo, Peralta Cuasolo, Yael Macarena, Valente, Carmen, Luini, Alberto, Corda, Daniela, Cáceres, Alfredo, Bisbal, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026897/
https://www.ncbi.nlm.nih.gov/pubmed/35455998
http://dx.doi.org/10.3390/cells11081320
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author Gastaldi, Laura
Martín, Josefina Inés
Sosa, Lucas Javier
Quassollo, Gonzalo
Peralta Cuasolo, Yael Macarena
Valente, Carmen
Luini, Alberto
Corda, Daniela
Cáceres, Alfredo
Bisbal, Mariano
author_facet Gastaldi, Laura
Martín, Josefina Inés
Sosa, Lucas Javier
Quassollo, Gonzalo
Peralta Cuasolo, Yael Macarena
Valente, Carmen
Luini, Alberto
Corda, Daniela
Cáceres, Alfredo
Bisbal, Mariano
author_sort Gastaldi, Laura
collection PubMed
description Neurons are highly polarized cells requiring precise regulation of trafficking and targeting of membrane proteins to generate and maintain different and specialized compartments, such as axons and dendrites. Disruption of the Golgi apparatus (GA) secretory pathway in developing neurons alters axon/dendritic formation. Therefore, detailed knowledge of the mechanisms underlying vesicles exiting from the GA is crucial for understanding neuronal polarity. In this study, we analyzed the role of Brefeldin A-Ribosylated Substrate (CtBP1-S/BARS), a member of the C-terminal-binding protein family, in the regulation of neuronal morphological polarization and the exit of membrane proteins from the Trans Golgi Network. Here, we show that BARS is expressed during neuronal development in vitro and that RNAi suppression of BARS inhibits axonal and dendritic elongation in hippocampal neuronal cultures as well as largely perturbed neuronal migration and multipolar-to-bipolar transition during cortical development in situ. In addition, using plasma membrane (PM) proteins fused to GFP and engineered with reversible aggregation domains, we observed that expression of fission dominant-negative BARS delays the exit of dendritic and axonal membrane protein-containing carriers from the GA. Taken together, these data provide the first set of evidence suggesting a role for BARS in neuronal development by regulating post-Golgi membrane trafficking.
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spelling pubmed-90268972022-04-23 BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking Gastaldi, Laura Martín, Josefina Inés Sosa, Lucas Javier Quassollo, Gonzalo Peralta Cuasolo, Yael Macarena Valente, Carmen Luini, Alberto Corda, Daniela Cáceres, Alfredo Bisbal, Mariano Cells Article Neurons are highly polarized cells requiring precise regulation of trafficking and targeting of membrane proteins to generate and maintain different and specialized compartments, such as axons and dendrites. Disruption of the Golgi apparatus (GA) secretory pathway in developing neurons alters axon/dendritic formation. Therefore, detailed knowledge of the mechanisms underlying vesicles exiting from the GA is crucial for understanding neuronal polarity. In this study, we analyzed the role of Brefeldin A-Ribosylated Substrate (CtBP1-S/BARS), a member of the C-terminal-binding protein family, in the regulation of neuronal morphological polarization and the exit of membrane proteins from the Trans Golgi Network. Here, we show that BARS is expressed during neuronal development in vitro and that RNAi suppression of BARS inhibits axonal and dendritic elongation in hippocampal neuronal cultures as well as largely perturbed neuronal migration and multipolar-to-bipolar transition during cortical development in situ. In addition, using plasma membrane (PM) proteins fused to GFP and engineered with reversible aggregation domains, we observed that expression of fission dominant-negative BARS delays the exit of dendritic and axonal membrane protein-containing carriers from the GA. Taken together, these data provide the first set of evidence suggesting a role for BARS in neuronal development by regulating post-Golgi membrane trafficking. MDPI 2022-04-13 /pmc/articles/PMC9026897/ /pubmed/35455998 http://dx.doi.org/10.3390/cells11081320 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gastaldi, Laura
Martín, Josefina Inés
Sosa, Lucas Javier
Quassollo, Gonzalo
Peralta Cuasolo, Yael Macarena
Valente, Carmen
Luini, Alberto
Corda, Daniela
Cáceres, Alfredo
Bisbal, Mariano
BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking
title BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking
title_full BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking
title_fullStr BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking
title_full_unstemmed BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking
title_short BARS Influences Neuronal Development by Regulation of Post-Golgi Trafficking
title_sort bars influences neuronal development by regulation of post-golgi trafficking
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026897/
https://www.ncbi.nlm.nih.gov/pubmed/35455998
http://dx.doi.org/10.3390/cells11081320
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