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Investigation of SARS-CoV-2 presence on environmental surfaces and waste in healthcare and non-healthcare facilities

OBJECTIVE: The objective of the paper is to investigate the presence of SARS-CoV-2 on inanimate surfaces in four healthcare facilities treating patients with COVID-19 and four quarantine regiments of provincial military commands. METHODS: From August to October 2020, a total of 468 one-off environme...

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Detalles Bibliográficos
Autores principales: Mai, Le Thi Phuong, Tran, Van Dinh, Phuong, Hoang Vu Mai, Trang, Ung Thi Hong, Thanh, Le Thi, Son, Nguyen Vu, Cuong, Vuong Duc, Dung, Luu Phuong, Hanh, Nguyen Thi My, Hai, Hoang, Oanh, Dang Thi Kieu, Thuy, Nguyen Thanh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026952/
https://www.ncbi.nlm.nih.gov/pubmed/37519307
http://dx.doi.org/10.1016/j.envc.2022.100526
Descripción
Sumario:OBJECTIVE: The objective of the paper is to investigate the presence of SARS-CoV-2 on inanimate surfaces in four healthcare facilities treating patients with COVID-19 and four quarantine regiments of provincial military commands. METHODS: From August to October 2020, a total of 468 one-off environmental samples consisting of inanimate surfaces, garbage, and wastewater were collected. The real-time RT-PCR assay targeting E and RdRp genes to detect SARS-CoV-2 and checklist and questionnaire of disinfection practices were employed. If detected by RT-PCR, then positive samples are subjected to cell culture to determine viability. RESULTS: The test results showed all samples (100%) to be negative with SARS-CoV-2 resulting in unperformed virus culture. As for recent disinfection practices, chlorine-based products dissolved at a concentration of 0.1% (1000 ppm) in the general context or 0.5% (5000 ppm) for blood and body fluid spills are routinely applied twice a day and at the discharge of patients or quarantined people. CONCLUSIONS: The finding may illustrate the importance of disinfection practices in removing pathogens or significantly reducing SARS-CoV-2 contamination on environmental surfaces and waste.