Cargando…
Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM)
The present work is conducted to investigate the optimal extraction technology of polysaccharide from chestnut mushroom (Agrocybe aegerita) using a new method based on accelerated solvent extraction combined with response surface methodology (ASE-RSM). The conventional reflux extraction (CRE) method...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027027/ https://www.ncbi.nlm.nih.gov/pubmed/35458578 http://dx.doi.org/10.3390/molecules27082380 |
_version_ | 1784691257752158208 |
---|---|
author | Du, Bin Peng, Fei Xie, Ying Wang, Huiying Wu, Jinhui Liu, Chang Yang, Yuedong |
author_facet | Du, Bin Peng, Fei Xie, Ying Wang, Huiying Wu, Jinhui Liu, Chang Yang, Yuedong |
author_sort | Du, Bin |
collection | PubMed |
description | The present work is conducted to investigate the optimal extraction technology of polysaccharide from chestnut mushroom (Agrocybe aegerita) using a new method based on accelerated solvent extraction combined with response surface methodology (ASE-RSM). The conventional reflux extraction (CRE) method and ultrasonic-assisted extraction (UAE) method were also carried out. Additionally, the in vitro antioxidant activities, including ABTS and DPPH assay, were evaluated. The RSM method, based on a three level and three variable Box–Behnken design (BBD), was developed to obtain the optimal combination of extraction conditions. In brief, the polysaccharide was optimally extracted with water as extraction solvent, extraction temperature of 71 °C, extraction time of 6.5 min, number of cycles of 3, and extraction pressure of 10 MPa. The 3D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under the above conditions, the experimental value of polysaccharide yield was 19.77 ± 0.12%, which is in close agreement with the value (19.81%) predicted by the model. These findings demonstrate that ASE-RSM produce much higher polysaccharide and consumed environmentally friendly extraction and solvent systems, have less extraction discrimination and shorter time and provide scientific basis for industrialization of polysaccharide extraction. Moreover, it was proved that the polysaccharide had the potential ability to scavenge ABTS and DPPH. |
format | Online Article Text |
id | pubmed-9027027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90270272022-04-23 Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) Du, Bin Peng, Fei Xie, Ying Wang, Huiying Wu, Jinhui Liu, Chang Yang, Yuedong Molecules Article The present work is conducted to investigate the optimal extraction technology of polysaccharide from chestnut mushroom (Agrocybe aegerita) using a new method based on accelerated solvent extraction combined with response surface methodology (ASE-RSM). The conventional reflux extraction (CRE) method and ultrasonic-assisted extraction (UAE) method were also carried out. Additionally, the in vitro antioxidant activities, including ABTS and DPPH assay, were evaluated. The RSM method, based on a three level and three variable Box–Behnken design (BBD), was developed to obtain the optimal combination of extraction conditions. In brief, the polysaccharide was optimally extracted with water as extraction solvent, extraction temperature of 71 °C, extraction time of 6.5 min, number of cycles of 3, and extraction pressure of 10 MPa. The 3D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under the above conditions, the experimental value of polysaccharide yield was 19.77 ± 0.12%, which is in close agreement with the value (19.81%) predicted by the model. These findings demonstrate that ASE-RSM produce much higher polysaccharide and consumed environmentally friendly extraction and solvent systems, have less extraction discrimination and shorter time and provide scientific basis for industrialization of polysaccharide extraction. Moreover, it was proved that the polysaccharide had the potential ability to scavenge ABTS and DPPH. MDPI 2022-04-07 /pmc/articles/PMC9027027/ /pubmed/35458578 http://dx.doi.org/10.3390/molecules27082380 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Du, Bin Peng, Fei Xie, Ying Wang, Huiying Wu, Jinhui Liu, Chang Yang, Yuedong Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) |
title | Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) |
title_full | Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) |
title_fullStr | Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) |
title_full_unstemmed | Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) |
title_short | Optimization Extraction and Antioxidant Activity of Crude Polysaccharide from Chestnut Mushroom (Agrocybe aegerita) by Accelerated Solvent Extraction Combined with Response Surface Methodology (ASE-RSM) |
title_sort | optimization extraction and antioxidant activity of crude polysaccharide from chestnut mushroom (agrocybe aegerita) by accelerated solvent extraction combined with response surface methodology (ase-rsm) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027027/ https://www.ncbi.nlm.nih.gov/pubmed/35458578 http://dx.doi.org/10.3390/molecules27082380 |
work_keys_str_mv | AT dubin optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm AT pengfei optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm AT xieying optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm AT wanghuiying optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm AT wujinhui optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm AT liuchang optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm AT yangyuedong optimizationextractionandantioxidantactivityofcrudepolysaccharidefromchestnutmushroomagrocybeaegeritabyacceleratedsolventextractioncombinedwithresponsesurfacemethodologyasersm |