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ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis

3-ketoacyl-CoA synthases (KCSs), as components of a fatty acid elongase (FAE) complex, play key roles in determining the chain length of very-long-chain fatty acids (VLCFAs). KCS6, taking a predominate role during the elongation from C26 to C28, is well known to play an important role in wax synthes...

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Autores principales: Huang, Haodong, Ayaz, Asma, Zheng, Minglü, Yang, Xianpeng, Zaman, Wajid, Zhao, Huayan, Lü, Shiyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027390/
https://www.ncbi.nlm.nih.gov/pubmed/35457268
http://dx.doi.org/10.3390/ijms23084450
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author Huang, Haodong
Ayaz, Asma
Zheng, Minglü
Yang, Xianpeng
Zaman, Wajid
Zhao, Huayan
Lü, Shiyou
author_facet Huang, Haodong
Ayaz, Asma
Zheng, Minglü
Yang, Xianpeng
Zaman, Wajid
Zhao, Huayan
Lü, Shiyou
author_sort Huang, Haodong
collection PubMed
description 3-ketoacyl-CoA synthases (KCSs), as components of a fatty acid elongase (FAE) complex, play key roles in determining the chain length of very-long-chain fatty acids (VLCFAs). KCS6, taking a predominate role during the elongation from C26 to C28, is well known to play an important role in wax synthesis. KCS5 is one paralog of KCS6 and its role in wax synthesis remains unknown. Wax phenotype analysis showed that in kcs5 mutants, the total amounts of wax components derived from carbon 32 (C32) and C34 were apparently decreased in leaves, and those of C26 to C32 derivatives were obviously decreased in flowers. Heterologous yeast expression analysis showed that KCS5 alone displayed specificity towards C24 to C28 acids, and its coordination with CER2 and CER26 catalyzed the elongation of acids exceeding C28, especially displaying higher activity towards C28 acids than KCS6. BiLC experiments identified that KCS5 physically interacts with CER2 and CER26. Wax phenotype analysis of different organs in kcs5 and kcs6 single or double mutants showed that KCS6 mutation causes greater effects on the wax synthesis than KCS5 mutation in the tested organs, and simultaneous repression of both protein activities caused additive effects, suggesting that during the wax biosynthesis process, KCS5 and KCS6 play redundant roles, among which KCS6 plays a major role. In addition, simultaneous mutations of two genes nearly block drought-induced wax production, indicating that the reactions catalyzed by KCS5 and KCS6 play a critical role in the wax biosynthesis in response to drought.
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spelling pubmed-90273902022-04-23 ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis Huang, Haodong Ayaz, Asma Zheng, Minglü Yang, Xianpeng Zaman, Wajid Zhao, Huayan Lü, Shiyou Int J Mol Sci Article 3-ketoacyl-CoA synthases (KCSs), as components of a fatty acid elongase (FAE) complex, play key roles in determining the chain length of very-long-chain fatty acids (VLCFAs). KCS6, taking a predominate role during the elongation from C26 to C28, is well known to play an important role in wax synthesis. KCS5 is one paralog of KCS6 and its role in wax synthesis remains unknown. Wax phenotype analysis showed that in kcs5 mutants, the total amounts of wax components derived from carbon 32 (C32) and C34 were apparently decreased in leaves, and those of C26 to C32 derivatives were obviously decreased in flowers. Heterologous yeast expression analysis showed that KCS5 alone displayed specificity towards C24 to C28 acids, and its coordination with CER2 and CER26 catalyzed the elongation of acids exceeding C28, especially displaying higher activity towards C28 acids than KCS6. BiLC experiments identified that KCS5 physically interacts with CER2 and CER26. Wax phenotype analysis of different organs in kcs5 and kcs6 single or double mutants showed that KCS6 mutation causes greater effects on the wax synthesis than KCS5 mutation in the tested organs, and simultaneous repression of both protein activities caused additive effects, suggesting that during the wax biosynthesis process, KCS5 and KCS6 play redundant roles, among which KCS6 plays a major role. In addition, simultaneous mutations of two genes nearly block drought-induced wax production, indicating that the reactions catalyzed by KCS5 and KCS6 play a critical role in the wax biosynthesis in response to drought. MDPI 2022-04-18 /pmc/articles/PMC9027390/ /pubmed/35457268 http://dx.doi.org/10.3390/ijms23084450 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Haodong
Ayaz, Asma
Zheng, Minglü
Yang, Xianpeng
Zaman, Wajid
Zhao, Huayan
Lü, Shiyou
ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis
title ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis
title_full ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis
title_fullStr ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis
title_full_unstemmed ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis
title_short ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis
title_sort arabidopsiskcs5 and kcs6 play redundant roles in wax synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027390/
https://www.ncbi.nlm.nih.gov/pubmed/35457268
http://dx.doi.org/10.3390/ijms23084450
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