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CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easie...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027422/ https://www.ncbi.nlm.nih.gov/pubmed/35457271 http://dx.doi.org/10.3390/ijms23084454 |
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author | Zegeye, Workie Anley Tsegaw, Mesfin Zhang, Yingxin Cao, Liyong |
author_facet | Zegeye, Workie Anley Tsegaw, Mesfin Zhang, Yingxin Cao, Liyong |
author_sort | Zegeye, Workie Anley |
collection | PubMed |
description | To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easier tool for genome editing in a variety of living organisms including rice. Genome editing has transformed agriculture by reducing biotic and abiotic stresses and increasing yield. Recently, genome editing technologies have been developed quickly in order to avoid the challenges that genetically modified crops face. Developing transgenic-free edited plants without introducing foreign DNA has received regulatory approval in a number of countries. Several ongoing efforts from various countries are rapidly expanding to adopt the innovations. This review covers the mechanisms of CRISPR/Cas9, comparisons of CRISPR/Cas9 with other gene-editing technologies—including newly emerged Cas variants—and focuses on CRISPR/Cas9-targeted genes for rice crop improvement. We have further highlighted CRISPR/Cas9 vector construction model design and different bioinformatics tools for target site selection. |
format | Online Article Text |
id | pubmed-9027422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90274222022-04-23 CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement Zegeye, Workie Anley Tsegaw, Mesfin Zhang, Yingxin Cao, Liyong Int J Mol Sci Review To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easier tool for genome editing in a variety of living organisms including rice. Genome editing has transformed agriculture by reducing biotic and abiotic stresses and increasing yield. Recently, genome editing technologies have been developed quickly in order to avoid the challenges that genetically modified crops face. Developing transgenic-free edited plants without introducing foreign DNA has received regulatory approval in a number of countries. Several ongoing efforts from various countries are rapidly expanding to adopt the innovations. This review covers the mechanisms of CRISPR/Cas9, comparisons of CRISPR/Cas9 with other gene-editing technologies—including newly emerged Cas variants—and focuses on CRISPR/Cas9-targeted genes for rice crop improvement. We have further highlighted CRISPR/Cas9 vector construction model design and different bioinformatics tools for target site selection. MDPI 2022-04-18 /pmc/articles/PMC9027422/ /pubmed/35457271 http://dx.doi.org/10.3390/ijms23084454 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Zegeye, Workie Anley Tsegaw, Mesfin Zhang, Yingxin Cao, Liyong CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
title | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
title_full | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
title_fullStr | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
title_full_unstemmed | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
title_short | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
title_sort | crispr-based genome editing: advancements and opportunities for rice improvement |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027422/ https://www.ncbi.nlm.nih.gov/pubmed/35457271 http://dx.doi.org/10.3390/ijms23084454 |
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