Cargando…

CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement

To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easie...

Descripción completa

Detalles Bibliográficos
Autores principales: Zegeye, Workie Anley, Tsegaw, Mesfin, Zhang, Yingxin, Cao, Liyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027422/
https://www.ncbi.nlm.nih.gov/pubmed/35457271
http://dx.doi.org/10.3390/ijms23084454
_version_ 1784691358851661824
author Zegeye, Workie Anley
Tsegaw, Mesfin
Zhang, Yingxin
Cao, Liyong
author_facet Zegeye, Workie Anley
Tsegaw, Mesfin
Zhang, Yingxin
Cao, Liyong
author_sort Zegeye, Workie Anley
collection PubMed
description To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easier tool for genome editing in a variety of living organisms including rice. Genome editing has transformed agriculture by reducing biotic and abiotic stresses and increasing yield. Recently, genome editing technologies have been developed quickly in order to avoid the challenges that genetically modified crops face. Developing transgenic-free edited plants without introducing foreign DNA has received regulatory approval in a number of countries. Several ongoing efforts from various countries are rapidly expanding to adopt the innovations. This review covers the mechanisms of CRISPR/Cas9, comparisons of CRISPR/Cas9 with other gene-editing technologies—including newly emerged Cas variants—and focuses on CRISPR/Cas9-targeted genes for rice crop improvement. We have further highlighted CRISPR/Cas9 vector construction model design and different bioinformatics tools for target site selection.
format Online
Article
Text
id pubmed-9027422
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90274222022-04-23 CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement Zegeye, Workie Anley Tsegaw, Mesfin Zhang, Yingxin Cao, Liyong Int J Mol Sci Review To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easier tool for genome editing in a variety of living organisms including rice. Genome editing has transformed agriculture by reducing biotic and abiotic stresses and increasing yield. Recently, genome editing technologies have been developed quickly in order to avoid the challenges that genetically modified crops face. Developing transgenic-free edited plants without introducing foreign DNA has received regulatory approval in a number of countries. Several ongoing efforts from various countries are rapidly expanding to adopt the innovations. This review covers the mechanisms of CRISPR/Cas9, comparisons of CRISPR/Cas9 with other gene-editing technologies—including newly emerged Cas variants—and focuses on CRISPR/Cas9-targeted genes for rice crop improvement. We have further highlighted CRISPR/Cas9 vector construction model design and different bioinformatics tools for target site selection. MDPI 2022-04-18 /pmc/articles/PMC9027422/ /pubmed/35457271 http://dx.doi.org/10.3390/ijms23084454 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zegeye, Workie Anley
Tsegaw, Mesfin
Zhang, Yingxin
Cao, Liyong
CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
title CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
title_full CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
title_fullStr CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
title_full_unstemmed CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
title_short CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
title_sort crispr-based genome editing: advancements and opportunities for rice improvement
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027422/
https://www.ncbi.nlm.nih.gov/pubmed/35457271
http://dx.doi.org/10.3390/ijms23084454
work_keys_str_mv AT zegeyeworkieanley crisprbasedgenomeeditingadvancementsandopportunitiesforriceimprovement
AT tsegawmesfin crisprbasedgenomeeditingadvancementsandopportunitiesforriceimprovement
AT zhangyingxin crisprbasedgenomeeditingadvancementsandopportunitiesforriceimprovement
AT caoliyong crisprbasedgenomeeditingadvancementsandopportunitiesforriceimprovement