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Are We Studying Non-Coding RNAs Correctly? Lessons from nc886

Non-coding RNAs (ncRNAs), such as microRNAs or long ncRNAs, have brought about a new paradigm in the regulation of gene expression. Sequencing technologies have detected transcripts with tremendous sensitivity and throughput and revealed that the majority of them lack protein-coding potential. Myria...

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Detalles Bibliográficos
Autor principal: Lee, Yong Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027504/
https://www.ncbi.nlm.nih.gov/pubmed/35457068
http://dx.doi.org/10.3390/ijms23084251
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author Lee, Yong Sun
author_facet Lee, Yong Sun
author_sort Lee, Yong Sun
collection PubMed
description Non-coding RNAs (ncRNAs), such as microRNAs or long ncRNAs, have brought about a new paradigm in the regulation of gene expression. Sequencing technologies have detected transcripts with tremendous sensitivity and throughput and revealed that the majority of them lack protein-coding potential. Myriad articles have investigated numerous ncRNAs and many of them claim that ncRNAs play gene-regulatory roles. However, it is questionable whether all these articles draw conclusions through cautious gain- and loss-of function experiments whose design was reasonably based on an ncRNA’s correct identity and features. In this review, these issues are discussed with a regulatory ncRNA, nc886, as an example case to represent cautions and guidelines when studying ncRNAs.
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spelling pubmed-90275042022-04-23 Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 Lee, Yong Sun Int J Mol Sci Review Non-coding RNAs (ncRNAs), such as microRNAs or long ncRNAs, have brought about a new paradigm in the regulation of gene expression. Sequencing technologies have detected transcripts with tremendous sensitivity and throughput and revealed that the majority of them lack protein-coding potential. Myriad articles have investigated numerous ncRNAs and many of them claim that ncRNAs play gene-regulatory roles. However, it is questionable whether all these articles draw conclusions through cautious gain- and loss-of function experiments whose design was reasonably based on an ncRNA’s correct identity and features. In this review, these issues are discussed with a regulatory ncRNA, nc886, as an example case to represent cautions and guidelines when studying ncRNAs. MDPI 2022-04-12 /pmc/articles/PMC9027504/ /pubmed/35457068 http://dx.doi.org/10.3390/ijms23084251 Text en © 2022 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lee, Yong Sun
Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
title Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
title_full Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
title_fullStr Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
title_full_unstemmed Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
title_short Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
title_sort are we studying non-coding rnas correctly? lessons from nc886
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027504/
https://www.ncbi.nlm.nih.gov/pubmed/35457068
http://dx.doi.org/10.3390/ijms23084251
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