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Are We Studying Non-Coding RNAs Correctly? Lessons from nc886
Non-coding RNAs (ncRNAs), such as microRNAs or long ncRNAs, have brought about a new paradigm in the regulation of gene expression. Sequencing technologies have detected transcripts with tremendous sensitivity and throughput and revealed that the majority of them lack protein-coding potential. Myria...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027504/ https://www.ncbi.nlm.nih.gov/pubmed/35457068 http://dx.doi.org/10.3390/ijms23084251 |
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author | Lee, Yong Sun |
author_facet | Lee, Yong Sun |
author_sort | Lee, Yong Sun |
collection | PubMed |
description | Non-coding RNAs (ncRNAs), such as microRNAs or long ncRNAs, have brought about a new paradigm in the regulation of gene expression. Sequencing technologies have detected transcripts with tremendous sensitivity and throughput and revealed that the majority of them lack protein-coding potential. Myriad articles have investigated numerous ncRNAs and many of them claim that ncRNAs play gene-regulatory roles. However, it is questionable whether all these articles draw conclusions through cautious gain- and loss-of function experiments whose design was reasonably based on an ncRNA’s correct identity and features. In this review, these issues are discussed with a regulatory ncRNA, nc886, as an example case to represent cautions and guidelines when studying ncRNAs. |
format | Online Article Text |
id | pubmed-9027504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90275042022-04-23 Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 Lee, Yong Sun Int J Mol Sci Review Non-coding RNAs (ncRNAs), such as microRNAs or long ncRNAs, have brought about a new paradigm in the regulation of gene expression. Sequencing technologies have detected transcripts with tremendous sensitivity and throughput and revealed that the majority of them lack protein-coding potential. Myriad articles have investigated numerous ncRNAs and many of them claim that ncRNAs play gene-regulatory roles. However, it is questionable whether all these articles draw conclusions through cautious gain- and loss-of function experiments whose design was reasonably based on an ncRNA’s correct identity and features. In this review, these issues are discussed with a regulatory ncRNA, nc886, as an example case to represent cautions and guidelines when studying ncRNAs. MDPI 2022-04-12 /pmc/articles/PMC9027504/ /pubmed/35457068 http://dx.doi.org/10.3390/ijms23084251 Text en © 2022 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Lee, Yong Sun Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 |
title | Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 |
title_full | Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 |
title_fullStr | Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 |
title_full_unstemmed | Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 |
title_short | Are We Studying Non-Coding RNAs Correctly? Lessons from nc886 |
title_sort | are we studying non-coding rnas correctly? lessons from nc886 |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027504/ https://www.ncbi.nlm.nih.gov/pubmed/35457068 http://dx.doi.org/10.3390/ijms23084251 |
work_keys_str_mv | AT leeyongsun arewestudyingnoncodingrnascorrectlylessonsfromnc886 |