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RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters

Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profil...

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Autores principales: Glotov, Andrey S., Zelenkova, Irina E., Vashukova, Elena S., Shuvalova, Anna R., Zolotareva, Alexandra D., Polev, Dmitrii E., Barbitoff, Yury A., Glotov, Oleg S., Sarana, Andrey M., Shcherbak, Sergey G., Rozina, Mariya A., Gogotova, Victoria L., Predeus, Alexander V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027771/
https://www.ncbi.nlm.nih.gov/pubmed/35456380
http://dx.doi.org/10.3390/genes13040574
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author Glotov, Andrey S.
Zelenkova, Irina E.
Vashukova, Elena S.
Shuvalova, Anna R.
Zolotareva, Alexandra D.
Polev, Dmitrii E.
Barbitoff, Yury A.
Glotov, Oleg S.
Sarana, Andrey M.
Shcherbak, Sergey G.
Rozina, Mariya A.
Gogotova, Victoria L.
Predeus, Alexander V.
author_facet Glotov, Andrey S.
Zelenkova, Irina E.
Vashukova, Elena S.
Shuvalova, Anna R.
Zolotareva, Alexandra D.
Polev, Dmitrii E.
Barbitoff, Yury A.
Glotov, Oleg S.
Sarana, Andrey M.
Shcherbak, Sergey G.
Rozina, Mariya A.
Gogotova, Victoria L.
Predeus, Alexander V.
author_sort Glotov, Andrey S.
collection PubMed
description Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profiling of whole blood. Seven elite female speed skaters were profiled on the 18th day of high-altitude adaptation. Whole blood RNA-seq before and after an intense 1 h skating bout was used to measure gene expression changes associated with exercise. In order to identify the genes specifically regulated at high altitudes, we have leveraged the data from eight previously published microarray datasets studying blood expression changes after exercise at sea level. Using cell type-specific signatures, we were able to deconvolute changes of cell type abundance from individual gene expression changes. Among these were PHOSPHO1, with a known role in erythropoiesis, and MARC1 with a role in endogenic NO metabolism. We find that platelet and erythrocyte counts uniquely respond to altitude exercise, while changes in neutrophils represent a more generic marker of intense exercise. Publicly available data from both single cell atlases and exercise-related blood profiling dramatically increases the value of whole blood RNA-seq for the dynamic evaluation of physiological changes in an athlete’s body.
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spelling pubmed-90277712022-04-23 RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters Glotov, Andrey S. Zelenkova, Irina E. Vashukova, Elena S. Shuvalova, Anna R. Zolotareva, Alexandra D. Polev, Dmitrii E. Barbitoff, Yury A. Glotov, Oleg S. Sarana, Andrey M. Shcherbak, Sergey G. Rozina, Mariya A. Gogotova, Victoria L. Predeus, Alexander V. Genes (Basel) Article Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profiling of whole blood. Seven elite female speed skaters were profiled on the 18th day of high-altitude adaptation. Whole blood RNA-seq before and after an intense 1 h skating bout was used to measure gene expression changes associated with exercise. In order to identify the genes specifically regulated at high altitudes, we have leveraged the data from eight previously published microarray datasets studying blood expression changes after exercise at sea level. Using cell type-specific signatures, we were able to deconvolute changes of cell type abundance from individual gene expression changes. Among these were PHOSPHO1, with a known role in erythropoiesis, and MARC1 with a role in endogenic NO metabolism. We find that platelet and erythrocyte counts uniquely respond to altitude exercise, while changes in neutrophils represent a more generic marker of intense exercise. Publicly available data from both single cell atlases and exercise-related blood profiling dramatically increases the value of whole blood RNA-seq for the dynamic evaluation of physiological changes in an athlete’s body. MDPI 2022-03-24 /pmc/articles/PMC9027771/ /pubmed/35456380 http://dx.doi.org/10.3390/genes13040574 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Glotov, Andrey S.
Zelenkova, Irina E.
Vashukova, Elena S.
Shuvalova, Anna R.
Zolotareva, Alexandra D.
Polev, Dmitrii E.
Barbitoff, Yury A.
Glotov, Oleg S.
Sarana, Andrey M.
Shcherbak, Sergey G.
Rozina, Mariya A.
Gogotova, Victoria L.
Predeus, Alexander V.
RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
title RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
title_full RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
title_fullStr RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
title_full_unstemmed RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
title_short RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
title_sort rna sequencing of whole blood defines the signature of high intensity exercise at altitude in elite speed skaters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027771/
https://www.ncbi.nlm.nih.gov/pubmed/35456380
http://dx.doi.org/10.3390/genes13040574
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