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Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding
Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027846/ https://www.ncbi.nlm.nih.gov/pubmed/35458446 http://dx.doi.org/10.3390/v14040717 |
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author | Murali, Shruthi Rustandi, Richard R. Zheng, Xiwei Payne, Anne Shang, Liang |
author_facet | Murali, Shruthi Rustandi, Richard R. Zheng, Xiwei Payne, Anne Shang, Liang |
author_sort | Murali, Shruthi |
collection | PubMed |
description | Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accurate characterization than that of a viral subunit-ligand interaction. This review aims to summarize and evaluate the uses of these technologies specifically in virus–ligand and virus-like particle–ligand binding cases to guide the field towards studies that apply these robust methods for whole virus-based studies. |
format | Online Article Text |
id | pubmed-9027846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90278462022-04-23 Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding Murali, Shruthi Rustandi, Richard R. Zheng, Xiwei Payne, Anne Shang, Liang Viruses Review Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accurate characterization than that of a viral subunit-ligand interaction. This review aims to summarize and evaluate the uses of these technologies specifically in virus–ligand and virus-like particle–ligand binding cases to guide the field towards studies that apply these robust methods for whole virus-based studies. MDPI 2022-03-29 /pmc/articles/PMC9027846/ /pubmed/35458446 http://dx.doi.org/10.3390/v14040717 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Murali, Shruthi Rustandi, Richard R. Zheng, Xiwei Payne, Anne Shang, Liang Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding |
title | Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding |
title_full | Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding |
title_fullStr | Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding |
title_full_unstemmed | Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding |
title_short | Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding |
title_sort | applications of surface plasmon resonance and biolayer interferometry for virus–ligand binding |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027846/ https://www.ncbi.nlm.nih.gov/pubmed/35458446 http://dx.doi.org/10.3390/v14040717 |
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