Reduction in Diarrhoea and Modulation of Intestinal Gene Expression in Pigs Allocated a Low Protein Diet without Medicinal Zinc Oxide Post-Weaning

SIMPLE SUMMARY: Post-weaning diarrhoea in pigs can be a challenge and medicinal zinc oxide can decrease the need for antibiotic treatment. Low protein diets decrease diarrhoea post-weaning and soy protein concentrate improves protein digestion when compared to a soybean meal based diet. The aim of this study was to test the effect of low protein diets with different protein sources as an alternative to medicinal zinc oxide. The study demonstrated that low protein diets can decrease diarrhoea-related antibiotic treatments but also reduces growth performance. Additionally, the study presented a difference in gut nutrient metabolism when feeding very low protein levels. ABSTRACT: Weaning comprises a challenging period for pigs, but dietary tools can be implemented to avoid excess antibiotics usage. Therefore, we tested the effect of a 17.6% crude protein (CP) diet on growth and diarrhoea and investigated the effect of a 15.5% CP diet post-weaning on transcriptomic responses, growth, and diarrhoea-related antibiotic treatments. At weaning, pigs were divided into five dietary treatment groups in a three-phase diet from weaning to 30 kg bodyweight. The diets included a positive control group (PC) with medicinal zinc oxide, a negative control group (NC), a 17.6% CP diet based on soy protein concentrate (SP), a 17.6% CP diet based on soybean meal (SB), and a 15.5% CP diet with additional amino acids (XLA). Growth performance and the occurrence of diarrhoea were similar between the SP and SB groups. The XLA pigs had a reduced weight gain and fewer antibiotics treatments caused by diarrhoea, as well as a reduced level of blood proteins. Intestinal tissue samples from the XLA pigs displayed decreased expression of genes involved in nutrient metabolism and immune responses relative to the PC group. In conclusion, a very low CP diet reduces antibiotics treatments, but also adapts gut nutrient metabolism and reduces growth performance.