Cargando…
Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia
Grapevine Pinot gris virus (GPGV) is a widespread grapevine pathogen associated with symptoms of leaf mottling and deformation. In order to study the distribution and genetic diversity of GPGV in Russia, we tested 1347 grapevine samples from 3 regions of Russia–the Krasnodar Krai, Stavropol Krai, an...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028157/ https://www.ncbi.nlm.nih.gov/pubmed/35448789 http://dx.doi.org/10.3390/plants11081061 |
_version_ | 1784691546965147648 |
---|---|
author | Shvets, Darya Vinogradova, Svetlana |
author_facet | Shvets, Darya Vinogradova, Svetlana |
author_sort | Shvets, Darya |
collection | PubMed |
description | Grapevine Pinot gris virus (GPGV) is a widespread grapevine pathogen associated with symptoms of leaf mottling and deformation. In order to study the distribution and genetic diversity of GPGV in Russia, we tested 1347 grapevine samples from 3 regions of Russia–the Krasnodar Krai, Stavropol Krai, and Republic of Crimea—using duplex real-time RT-PCR. GPGV was detected in 993 grapevines, both symptomatic and asymptomatic. In 119 isolates, we sequenced complete movement protein (MP) and coat protein (CP) genes of the GPGV genome. The percentage of identity of the obtained nucleotide MP/CP sequences with the closest isolates from the GenBank was 97.75–99.56%. A phylogenetic analysis showed that these Russian GPGV isolates are mainly grouped with previously described representative asymptomatic isolates. New post-translational modifications of the MP and CP at the positions of polymorphisms in the genomes of Russian isolates were predicted. The present work is the first study on the distribution and genetic diversity of GPGV in Russia. |
format | Online Article Text |
id | pubmed-9028157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90281572022-04-23 Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia Shvets, Darya Vinogradova, Svetlana Plants (Basel) Article Grapevine Pinot gris virus (GPGV) is a widespread grapevine pathogen associated with symptoms of leaf mottling and deformation. In order to study the distribution and genetic diversity of GPGV in Russia, we tested 1347 grapevine samples from 3 regions of Russia–the Krasnodar Krai, Stavropol Krai, and Republic of Crimea—using duplex real-time RT-PCR. GPGV was detected in 993 grapevines, both symptomatic and asymptomatic. In 119 isolates, we sequenced complete movement protein (MP) and coat protein (CP) genes of the GPGV genome. The percentage of identity of the obtained nucleotide MP/CP sequences with the closest isolates from the GenBank was 97.75–99.56%. A phylogenetic analysis showed that these Russian GPGV isolates are mainly grouped with previously described representative asymptomatic isolates. New post-translational modifications of the MP and CP at the positions of polymorphisms in the genomes of Russian isolates were predicted. The present work is the first study on the distribution and genetic diversity of GPGV in Russia. MDPI 2022-04-13 /pmc/articles/PMC9028157/ /pubmed/35448789 http://dx.doi.org/10.3390/plants11081061 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shvets, Darya Vinogradova, Svetlana Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia |
title | Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia |
title_full | Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia |
title_fullStr | Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia |
title_full_unstemmed | Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia |
title_short | Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia |
title_sort | occurrence and genetic characterization of grapevine pinot gris virus in russia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028157/ https://www.ncbi.nlm.nih.gov/pubmed/35448789 http://dx.doi.org/10.3390/plants11081061 |
work_keys_str_mv | AT shvetsdarya occurrenceandgeneticcharacterizationofgrapevinepinotgrisvirusinrussia AT vinogradovasvetlana occurrenceandgeneticcharacterizationofgrapevinepinotgrisvirusinrussia |