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Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)

Most anti-inflammatory drugs used nowadays have an excessive cost and their prolonged use has been connected with several injurious effects. Thus, the search for new anti-inflammatory agents is increasing. Lectins are carbohydrate-interacting proteins that can modulate immune response and the releas...

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Autores principales: de Siqueira Patriota, Leydianne Leite, de Brito Marques Ramos, Dalila, e Silva, Mariana Gama, dos Santos, Angela Caroline Lima Amorim, Silva, Yasmym Araújo, Paiva, Patrícia Maria Guedes, Pontual, Emmanuel Viana, de Albuquerque, Lidiane Pereira, Mendes, Rosemairy Luciane, Napoleão, Thiago Henrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028213/
https://www.ncbi.nlm.nih.gov/pubmed/35458359
http://dx.doi.org/10.3390/polym14081609
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author de Siqueira Patriota, Leydianne Leite
de Brito Marques Ramos, Dalila
e Silva, Mariana Gama
dos Santos, Angela Caroline Lima Amorim
Silva, Yasmym Araújo
Paiva, Patrícia Maria Guedes
Pontual, Emmanuel Viana
de Albuquerque, Lidiane Pereira
Mendes, Rosemairy Luciane
Napoleão, Thiago Henrique
author_facet de Siqueira Patriota, Leydianne Leite
de Brito Marques Ramos, Dalila
e Silva, Mariana Gama
dos Santos, Angela Caroline Lima Amorim
Silva, Yasmym Araújo
Paiva, Patrícia Maria Guedes
Pontual, Emmanuel Viana
de Albuquerque, Lidiane Pereira
Mendes, Rosemairy Luciane
Napoleão, Thiago Henrique
author_sort de Siqueira Patriota, Leydianne Leite
collection PubMed
description Most anti-inflammatory drugs used nowadays have an excessive cost and their prolonged use has been connected with several injurious effects. Thus, the search for new anti-inflammatory agents is increasing. Lectins are carbohydrate-interacting proteins that can modulate immune response and the release of inflammation mediators. The Microgramma vacciniifolia frond lectin (MvFL) was previously reported to be an immunomodulatory agent in vitro. This work aimed to evaluate the effects of MvFL on the in vivo inflammatory status in the carrageenan-induced peritonitis and paw edema, using female Swiss mice. The animals were pretreated intraperitoneally with MvFL (5 and 10 mg/kg). In the peritonitis assay, the total and differential migration of white blood cells was evaluated, as well as the levels of cytokines, nitric oxide (NO), and total proteins in the peritoneal fluid. In the paw edema evaluation, the paw volume was measured in the early (from 30 min–2 h) and late (3–4 h) phases of edema formation. MvFL (5 and 10 mg/kg) was efficient in reducing neutrophil infiltration, pro-inflammatory cytokines (IL-6, IL-17, and TNF-α), NO, and protein content in the peritoneal fluid. It also repressed the edema formation in the late phase of the assay. In conclusion, MvFL showed inhibitory effects in in vivo acute inflammation, which encouraged future studies exploiting its immunomodulatory ability.
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spelling pubmed-90282132022-04-23 Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL) de Siqueira Patriota, Leydianne Leite de Brito Marques Ramos, Dalila e Silva, Mariana Gama dos Santos, Angela Caroline Lima Amorim Silva, Yasmym Araújo Paiva, Patrícia Maria Guedes Pontual, Emmanuel Viana de Albuquerque, Lidiane Pereira Mendes, Rosemairy Luciane Napoleão, Thiago Henrique Polymers (Basel) Article Most anti-inflammatory drugs used nowadays have an excessive cost and their prolonged use has been connected with several injurious effects. Thus, the search for new anti-inflammatory agents is increasing. Lectins are carbohydrate-interacting proteins that can modulate immune response and the release of inflammation mediators. The Microgramma vacciniifolia frond lectin (MvFL) was previously reported to be an immunomodulatory agent in vitro. This work aimed to evaluate the effects of MvFL on the in vivo inflammatory status in the carrageenan-induced peritonitis and paw edema, using female Swiss mice. The animals were pretreated intraperitoneally with MvFL (5 and 10 mg/kg). In the peritonitis assay, the total and differential migration of white blood cells was evaluated, as well as the levels of cytokines, nitric oxide (NO), and total proteins in the peritoneal fluid. In the paw edema evaluation, the paw volume was measured in the early (from 30 min–2 h) and late (3–4 h) phases of edema formation. MvFL (5 and 10 mg/kg) was efficient in reducing neutrophil infiltration, pro-inflammatory cytokines (IL-6, IL-17, and TNF-α), NO, and protein content in the peritoneal fluid. It also repressed the edema formation in the late phase of the assay. In conclusion, MvFL showed inhibitory effects in in vivo acute inflammation, which encouraged future studies exploiting its immunomodulatory ability. MDPI 2022-04-15 /pmc/articles/PMC9028213/ /pubmed/35458359 http://dx.doi.org/10.3390/polym14081609 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Siqueira Patriota, Leydianne Leite
de Brito Marques Ramos, Dalila
e Silva, Mariana Gama
dos Santos, Angela Caroline Lima Amorim
Silva, Yasmym Araújo
Paiva, Patrícia Maria Guedes
Pontual, Emmanuel Viana
de Albuquerque, Lidiane Pereira
Mendes, Rosemairy Luciane
Napoleão, Thiago Henrique
Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)
title Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)
title_full Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)
title_fullStr Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)
title_full_unstemmed Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)
title_short Inhibition of Carrageenan-Induced Acute Inflammation in Mice by the Microgramma vacciniifolia Frond Lectin (MvFL)
title_sort inhibition of carrageenan-induced acute inflammation in mice by the microgramma vacciniifolia frond lectin (mvfl)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028213/
https://www.ncbi.nlm.nih.gov/pubmed/35458359
http://dx.doi.org/10.3390/polym14081609
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