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TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15

Background: CTX-M betalactamases have shown a rapid spread in the recent years among Enterobacteriaceae and have become the most prevalent Extended Spectrum Beta-Lactamases (ESBLs) in many parts of the world. The introduction and dissemination of antibiotic-resistant genes limits options for treatme...

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Autores principales: Ghenea, Alice Elena, Zlatian, Ovidiu Mircea, Cristea, Oana Mariana, Ungureanu, Anca, Mititelu, Radu Razvan, Balasoiu, Andrei Theodor, Vasile, Corina Maria, Salan, Alex-Ioan, Iliuta, Daniel, Popescu, Mihaela, Udriștoiu, Anca-Loredana, Balasoiu, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028254/
https://www.ncbi.nlm.nih.gov/pubmed/35453254
http://dx.doi.org/10.3390/antibiotics11040503
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author Ghenea, Alice Elena
Zlatian, Ovidiu Mircea
Cristea, Oana Mariana
Ungureanu, Anca
Mititelu, Radu Razvan
Balasoiu, Andrei Theodor
Vasile, Corina Maria
Salan, Alex-Ioan
Iliuta, Daniel
Popescu, Mihaela
Udriștoiu, Anca-Loredana
Balasoiu, Maria
author_facet Ghenea, Alice Elena
Zlatian, Ovidiu Mircea
Cristea, Oana Mariana
Ungureanu, Anca
Mititelu, Radu Razvan
Balasoiu, Andrei Theodor
Vasile, Corina Maria
Salan, Alex-Ioan
Iliuta, Daniel
Popescu, Mihaela
Udriștoiu, Anca-Loredana
Balasoiu, Maria
author_sort Ghenea, Alice Elena
collection PubMed
description Background: CTX-M betalactamases have shown a rapid spread in the recent years among Enterobacteriaceae and have become the most prevalent Extended Spectrum Beta-Lactamases (ESBLs) in many parts of the world. The introduction and dissemination of antibiotic-resistant genes limits options for treatment, increases mortality and morbidity in patients, and leads to longer hospitalization and expensive costs. We aimed to identify the beta-lactamases circulating encoded by the genes bla(CTX-M-15), bla(SHV-1) and bla(TEM-1) in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains. Furthermore, we established the associated resistance phenotypes among patients hospitalized in the Intensive Care Unit (ICU) from County Clinical Emergency Hospital of Craiova, Romania. Methods: A total of 46 non-duplicated bacterial strains (14 strains of E. coli and 32 strains of K. pneumoniae), which were resistant to ceftazidime (CAZ) and cefotaxime (CTX) by Kirby–Bauer disk diffusion method, were identified using the automated VITEK2 system. Detection of ESBL-encoding genes and other resistance genes was carried out by PCR. Results. E. coli strains were resistant to 3rd generation cephalosporins and moderately resistant to quinolones, whereas K. pneumoniae strains were resistant to penicillins, cephalosporins, and sulfamides, and moderately resistant to quinolones and carbapenems. Most E. coli strains harbored bla(CTX-M-15) gene (13/14 strains), a single strain had the bla(SHV-1) gene, but 11 strains harbored bla(TEM-1) gene. The mcr-1 gene was not detected. We detected tet(A) gene in six strains and tet(B) in one strain. In K. pneumoniae strains we detected bla(CTX-M-15) in 23 strains, bla(SHV)-(1) in all strains and bla(TEM-1) in 14 strains. The colistin resistance gene mcr-1 was not detected. The tetracycline gene tet(A) was detected in 11 strains, but the gene tet(B) was not detected in any strains. Conclusions. The development in antibiotic resistance highlights the importance of establishing policies to reduce antibiotic use and improving the national resistance surveillance system in order to create local antibiotic therapy guidelines.
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spelling pubmed-90282542022-04-23 TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 Ghenea, Alice Elena Zlatian, Ovidiu Mircea Cristea, Oana Mariana Ungureanu, Anca Mititelu, Radu Razvan Balasoiu, Andrei Theodor Vasile, Corina Maria Salan, Alex-Ioan Iliuta, Daniel Popescu, Mihaela Udriștoiu, Anca-Loredana Balasoiu, Maria Antibiotics (Basel) Article Background: CTX-M betalactamases have shown a rapid spread in the recent years among Enterobacteriaceae and have become the most prevalent Extended Spectrum Beta-Lactamases (ESBLs) in many parts of the world. The introduction and dissemination of antibiotic-resistant genes limits options for treatment, increases mortality and morbidity in patients, and leads to longer hospitalization and expensive costs. We aimed to identify the beta-lactamases circulating encoded by the genes bla(CTX-M-15), bla(SHV-1) and bla(TEM-1) in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains. Furthermore, we established the associated resistance phenotypes among patients hospitalized in the Intensive Care Unit (ICU) from County Clinical Emergency Hospital of Craiova, Romania. Methods: A total of 46 non-duplicated bacterial strains (14 strains of E. coli and 32 strains of K. pneumoniae), which were resistant to ceftazidime (CAZ) and cefotaxime (CTX) by Kirby–Bauer disk diffusion method, were identified using the automated VITEK2 system. Detection of ESBL-encoding genes and other resistance genes was carried out by PCR. Results. E. coli strains were resistant to 3rd generation cephalosporins and moderately resistant to quinolones, whereas K. pneumoniae strains were resistant to penicillins, cephalosporins, and sulfamides, and moderately resistant to quinolones and carbapenems. Most E. coli strains harbored bla(CTX-M-15) gene (13/14 strains), a single strain had the bla(SHV-1) gene, but 11 strains harbored bla(TEM-1) gene. The mcr-1 gene was not detected. We detected tet(A) gene in six strains and tet(B) in one strain. In K. pneumoniae strains we detected bla(CTX-M-15) in 23 strains, bla(SHV)-(1) in all strains and bla(TEM-1) in 14 strains. The colistin resistance gene mcr-1 was not detected. The tetracycline gene tet(A) was detected in 11 strains, but the gene tet(B) was not detected in any strains. Conclusions. The development in antibiotic resistance highlights the importance of establishing policies to reduce antibiotic use and improving the national resistance surveillance system in order to create local antibiotic therapy guidelines. MDPI 2022-04-10 /pmc/articles/PMC9028254/ /pubmed/35453254 http://dx.doi.org/10.3390/antibiotics11040503 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghenea, Alice Elena
Zlatian, Ovidiu Mircea
Cristea, Oana Mariana
Ungureanu, Anca
Mititelu, Radu Razvan
Balasoiu, Andrei Theodor
Vasile, Corina Maria
Salan, Alex-Ioan
Iliuta, Daniel
Popescu, Mihaela
Udriștoiu, Anca-Loredana
Balasoiu, Maria
TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
title TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
title_full TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
title_fullStr TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
title_full_unstemmed TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
title_short TEM,CTX-M,SHV Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
title_sort tem,ctx-m,shv genes in esbl-producing escherichia coli and klebsiella pneumoniae isolated from clinical samples in a county clinical emergency hospital romania-predominance of ctx-m-15
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028254/
https://www.ncbi.nlm.nih.gov/pubmed/35453254
http://dx.doi.org/10.3390/antibiotics11040503
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