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Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology

The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example...

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Autores principales: Rodrigo, Ana P., Lopes, Ana, Pereira, Ricardo, Anjo, Sandra I., Manadas, Bruno, Grosso, Ana R., Baptista, Pedro V., Fernandes, Alexandra R., Costa, Pedro M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028460/
https://www.ncbi.nlm.nih.gov/pubmed/35447897
http://dx.doi.org/10.3390/md20040224
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author Rodrigo, Ana P.
Lopes, Ana
Pereira, Ricardo
Anjo, Sandra I.
Manadas, Bruno
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
author_facet Rodrigo, Ana P.
Lopes, Ana
Pereira, Ricardo
Anjo, Sandra I.
Manadas, Bruno
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
author_sort Rodrigo, Ana P.
collection PubMed
description The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue–greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers.
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spelling pubmed-90284602022-04-23 Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology Rodrigo, Ana P. Lopes, Ana Pereira, Ricardo Anjo, Sandra I. Manadas, Bruno Grosso, Ana R. Baptista, Pedro V. Fernandes, Alexandra R. Costa, Pedro M. Mar Drugs Article The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue–greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers. MDPI 2022-03-25 /pmc/articles/PMC9028460/ /pubmed/35447897 http://dx.doi.org/10.3390/md20040224 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodrigo, Ana P.
Lopes, Ana
Pereira, Ricardo
Anjo, Sandra I.
Manadas, Bruno
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
title Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
title_full Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
title_fullStr Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
title_full_unstemmed Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
title_short Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
title_sort endogenous fluorescent proteins in the mucus of an intertidal polychaeta: clues for biotechnology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028460/
https://www.ncbi.nlm.nih.gov/pubmed/35447897
http://dx.doi.org/10.3390/md20040224
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