Cargando…

Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections

Reticuloendotheliosis virus (REV) is a retroviral pathogen capable of infecting several avian hosts and is associated with immunosuppression, anemia, proventriculitis, neoplasia, and runting–stunting syndrome. Its genome contains the three major genes, gag, pol, and env, and two flanking long termin...

Descripción completa

Detalles Bibliográficos
Autores principales: Chacón, Ruy D., Sedano-Herrera, Benjy, Alfaro-Espinoza, Elizabeth Regina, Quispe, Wilma Ursula, Liñan-Torres, Arturo, De la Torre, David, de Oliveira, Anderson, Astolfi-Ferreira, Claudete S., Ferreira, Antonio J. Piantino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028558/
https://www.ncbi.nlm.nih.gov/pubmed/35458529
http://dx.doi.org/10.3390/v14040798
_version_ 1784691649499103232
author Chacón, Ruy D.
Sedano-Herrera, Benjy
Alfaro-Espinoza, Elizabeth Regina
Quispe, Wilma Ursula
Liñan-Torres, Arturo
De la Torre, David
de Oliveira, Anderson
Astolfi-Ferreira, Claudete S.
Ferreira, Antonio J. Piantino
author_facet Chacón, Ruy D.
Sedano-Herrera, Benjy
Alfaro-Espinoza, Elizabeth Regina
Quispe, Wilma Ursula
Liñan-Torres, Arturo
De la Torre, David
de Oliveira, Anderson
Astolfi-Ferreira, Claudete S.
Ferreira, Antonio J. Piantino
author_sort Chacón, Ruy D.
collection PubMed
description Reticuloendotheliosis virus (REV) is a retroviral pathogen capable of infecting several avian hosts and is associated with immunosuppression, anemia, proventriculitis, neoplasia, and runting–stunting syndrome. Its genome contains the three major genes, gag, pol, and env, and two flanking long terminal repeat (LTR) regions. Complete genome sequences of REV are limited in terms of geographical origin. The aim of this study was to characterize the complete genome of REV detected in Brazilian chickens with multiple viral coinfections and analyze the polymorphisms in the deduced amino acids sequences corresponding to its encoded proteins. We tested the presence and completeness of REV as well as other viral pathogens in samples from Brazilian poultry farms by qPCR. The complete genomes of two REV strains were sequenced by overlapping fragments through the dideoxy method. Phylogenetic analysis, pairwise identity matrix, polymorphism identification and protein modeling were performed along the entire genome. We detected REV in 65% (26/40) of the tested samples. Concomitant viral infections were detected in 82.5% (33/40) of the samples and in 90% (9/10) of the farms. Multiple infections included up to seven viruses. Phylogenetic analysis classified both Brazilian strains into REV subtype 3, and the pairwise comparison indicated that strains from the USA and fowlpox virus (FWPV)-related strains were the most identical. The subdomain p18 in gag, the reverse transcriptase/ribonuclease H in pol, and the surface (SU) in the env protein were the most polymorphic in genomic comparisons. The relevant motifs for each protein were highly conserved, with fewer polymorphisms in the fusion peptide, immunosuppression domain, and disulfide bonds on the surface (SU) and transmembrane (TM) of env. This is the first study to include complete genomes of REV in Brazil and South America detected in farms with multiple viral coinfections. Our findings suggest an involvement of REV as an immunosuppressor and active agent in the emergence and progression of multiple infectious diseases. We also found a possible etiological relationship between Brazilian strains and the USA and FWPV recombinant strains. This information highlights the need for epidemiological vigilance regarding REV in association with another pathogens.
format Online
Article
Text
id pubmed-9028558
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90285582022-04-23 Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections Chacón, Ruy D. Sedano-Herrera, Benjy Alfaro-Espinoza, Elizabeth Regina Quispe, Wilma Ursula Liñan-Torres, Arturo De la Torre, David de Oliveira, Anderson Astolfi-Ferreira, Claudete S. Ferreira, Antonio J. Piantino Viruses Article Reticuloendotheliosis virus (REV) is a retroviral pathogen capable of infecting several avian hosts and is associated with immunosuppression, anemia, proventriculitis, neoplasia, and runting–stunting syndrome. Its genome contains the three major genes, gag, pol, and env, and two flanking long terminal repeat (LTR) regions. Complete genome sequences of REV are limited in terms of geographical origin. The aim of this study was to characterize the complete genome of REV detected in Brazilian chickens with multiple viral coinfections and analyze the polymorphisms in the deduced amino acids sequences corresponding to its encoded proteins. We tested the presence and completeness of REV as well as other viral pathogens in samples from Brazilian poultry farms by qPCR. The complete genomes of two REV strains were sequenced by overlapping fragments through the dideoxy method. Phylogenetic analysis, pairwise identity matrix, polymorphism identification and protein modeling were performed along the entire genome. We detected REV in 65% (26/40) of the tested samples. Concomitant viral infections were detected in 82.5% (33/40) of the samples and in 90% (9/10) of the farms. Multiple infections included up to seven viruses. Phylogenetic analysis classified both Brazilian strains into REV subtype 3, and the pairwise comparison indicated that strains from the USA and fowlpox virus (FWPV)-related strains were the most identical. The subdomain p18 in gag, the reverse transcriptase/ribonuclease H in pol, and the surface (SU) in the env protein were the most polymorphic in genomic comparisons. The relevant motifs for each protein were highly conserved, with fewer polymorphisms in the fusion peptide, immunosuppression domain, and disulfide bonds on the surface (SU) and transmembrane (TM) of env. This is the first study to include complete genomes of REV in Brazil and South America detected in farms with multiple viral coinfections. Our findings suggest an involvement of REV as an immunosuppressor and active agent in the emergence and progression of multiple infectious diseases. We also found a possible etiological relationship between Brazilian strains and the USA and FWPV recombinant strains. This information highlights the need for epidemiological vigilance regarding REV in association with another pathogens. MDPI 2022-04-13 /pmc/articles/PMC9028558/ /pubmed/35458529 http://dx.doi.org/10.3390/v14040798 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chacón, Ruy D.
Sedano-Herrera, Benjy
Alfaro-Espinoza, Elizabeth Regina
Quispe, Wilma Ursula
Liñan-Torres, Arturo
De la Torre, David
de Oliveira, Anderson
Astolfi-Ferreira, Claudete S.
Ferreira, Antonio J. Piantino
Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections
title Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections
title_full Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections
title_fullStr Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections
title_full_unstemmed Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections
title_short Complete Genome Characterization of Reticuloendotheliosis Virus Detected in Chickens with Multiple Viral Coinfections
title_sort complete genome characterization of reticuloendotheliosis virus detected in chickens with multiple viral coinfections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028558/
https://www.ncbi.nlm.nih.gov/pubmed/35458529
http://dx.doi.org/10.3390/v14040798
work_keys_str_mv AT chaconruyd completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT sedanoherrerabenjy completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT alfaroespinozaelizabethregina completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT quispewilmaursula completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT linantorresarturo completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT delatorredavid completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT deoliveiraanderson completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT astolfiferreiraclaudetes completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections
AT ferreiraantoniojpiantino completegenomecharacterizationofreticuloendotheliosisvirusdetectedinchickenswithmultipleviralcoinfections