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Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota

Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomo...

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Autores principales: Vlassopoulou, Marigoula, Paschalidis, Nikolaos, Savvides, Alexandros L., Saxami, Georgia, Mitsou, Evdokia K., Kerezoudi, Evangelia N., Koutrotsios, Georgios, Zervakis, Georgios I., Georgiadis, Panagiotis, Kyriacou, Adamantini, Pletsa, Vasiliki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028658/
https://www.ncbi.nlm.nih.gov/pubmed/35448559
http://dx.doi.org/10.3390/jof8040329
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author Vlassopoulou, Marigoula
Paschalidis, Nikolaos
Savvides, Alexandros L.
Saxami, Georgia
Mitsou, Evdokia K.
Kerezoudi, Evangelia N.
Koutrotsios, Georgios
Zervakis, Georgios I.
Georgiadis, Panagiotis
Kyriacou, Adamantini
Pletsa, Vasiliki
author_facet Vlassopoulou, Marigoula
Paschalidis, Nikolaos
Savvides, Alexandros L.
Saxami, Georgia
Mitsou, Evdokia K.
Kerezoudi, Evangelia N.
Koutrotsios, Georgios
Zervakis, Georgios I.
Georgiadis, Panagiotis
Kyriacou, Adamantini
Pletsa, Vasiliki
author_sort Vlassopoulou, Marigoula
collection PubMed
description Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. We investigated the immunomodulating effects of Pleurotus eryngii mushrooms, selected due to their high β-glucan content, strong lactogenic effect, and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers (>60 years old). The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages. Gene expression as well as pro- (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-10, IL-1Rα) were assessed and correlated with the fermentation process. The presence of P. eryngii in the fermentation process led to modifications in immune response, as indicated by the altered gene expression and levels of the cytokines examined, a finding consistent for all volunteers. The FSs immunomodulating effect on the volunteers’ peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis.
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spelling pubmed-90286582022-04-23 Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota Vlassopoulou, Marigoula Paschalidis, Nikolaos Savvides, Alexandros L. Saxami, Georgia Mitsou, Evdokia K. Kerezoudi, Evangelia N. Koutrotsios, Georgios Zervakis, Georgios I. Georgiadis, Panagiotis Kyriacou, Adamantini Pletsa, Vasiliki J Fungi (Basel) Article Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. We investigated the immunomodulating effects of Pleurotus eryngii mushrooms, selected due to their high β-glucan content, strong lactogenic effect, and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers (>60 years old). The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages. Gene expression as well as pro- (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-10, IL-1Rα) were assessed and correlated with the fermentation process. The presence of P. eryngii in the fermentation process led to modifications in immune response, as indicated by the altered gene expression and levels of the cytokines examined, a finding consistent for all volunteers. The FSs immunomodulating effect on the volunteers’ peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis. MDPI 2022-03-22 /pmc/articles/PMC9028658/ /pubmed/35448559 http://dx.doi.org/10.3390/jof8040329 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vlassopoulou, Marigoula
Paschalidis, Nikolaos
Savvides, Alexandros L.
Saxami, Georgia
Mitsou, Evdokia K.
Kerezoudi, Evangelia N.
Koutrotsios, Georgios
Zervakis, Georgios I.
Georgiadis, Panagiotis
Kyriacou, Adamantini
Pletsa, Vasiliki
Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
title Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
title_full Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
title_fullStr Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
title_full_unstemmed Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
title_short Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
title_sort immunomodulating activity of pleurotus eryngii mushrooms following their in vitro fermentation by human fecal microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028658/
https://www.ncbi.nlm.nih.gov/pubmed/35448559
http://dx.doi.org/10.3390/jof8040329
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