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Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives

Most of the research on bioremediation and estimation of microbial diversity in waste contaminated sites is focused on the domain Bacteria, whereas details on the relevance of Archaea are still lacking. The present study examined the archaeal diversity and predicted metabolic pathways in two discret...

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Autores principales: Pal, Yash, Mayilraj, Shanmugam, Krishnamurthi, Srinivasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028785/
https://www.ncbi.nlm.nih.gov/pubmed/35454078
http://dx.doi.org/10.3390/biom12040489
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author Pal, Yash
Mayilraj, Shanmugam
Krishnamurthi, Srinivasan
author_facet Pal, Yash
Mayilraj, Shanmugam
Krishnamurthi, Srinivasan
author_sort Pal, Yash
collection PubMed
description Most of the research on bioremediation and estimation of microbial diversity in waste contaminated sites is focused on the domain Bacteria, whereas details on the relevance of Archaea are still lacking. The present study examined the archaeal diversity and predicted metabolic pathways in two discrete sites (SITE1 and SITE2) contaminated with explosives (RDX and HMX) by amplicon-targeted sequencing of 16S rRNA genes. In total, 14 soil samples were processed, and 35,758 OTUs were observed, among which 981 OTUs were classified as Archaea, representing ~2.7% of the total microbial diversity in our samples. The majority of OTUs belonged to phyla Euryarchaeota (~49%), Crenarchaeota (~24%), and Thaumarchaeota (~23%), while the remaining (~4%) OTUs were affiliated to Candidatus Parvarchaeota, Candidatus Aenigmarchaeota, and Candidatus Diapherotrites. The comparative studies between explosives contaminated and agricultural soil samples (with no history of explosives contamination) displayed significant differences between the compositions of the archaeal communities. Further, the metabolic pathways pertaining to xenobiotic degradation were presumably more abundant in the contaminated sites. Our data provide a first comprehensive report of archaeal communities in explosives contaminated sites and their putative degradation role in such ecosystems which have been as yet unexplored.
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spelling pubmed-90287852022-04-23 Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives Pal, Yash Mayilraj, Shanmugam Krishnamurthi, Srinivasan Biomolecules Article Most of the research on bioremediation and estimation of microbial diversity in waste contaminated sites is focused on the domain Bacteria, whereas details on the relevance of Archaea are still lacking. The present study examined the archaeal diversity and predicted metabolic pathways in two discrete sites (SITE1 and SITE2) contaminated with explosives (RDX and HMX) by amplicon-targeted sequencing of 16S rRNA genes. In total, 14 soil samples were processed, and 35,758 OTUs were observed, among which 981 OTUs were classified as Archaea, representing ~2.7% of the total microbial diversity in our samples. The majority of OTUs belonged to phyla Euryarchaeota (~49%), Crenarchaeota (~24%), and Thaumarchaeota (~23%), while the remaining (~4%) OTUs were affiliated to Candidatus Parvarchaeota, Candidatus Aenigmarchaeota, and Candidatus Diapherotrites. The comparative studies between explosives contaminated and agricultural soil samples (with no history of explosives contamination) displayed significant differences between the compositions of the archaeal communities. Further, the metabolic pathways pertaining to xenobiotic degradation were presumably more abundant in the contaminated sites. Our data provide a first comprehensive report of archaeal communities in explosives contaminated sites and their putative degradation role in such ecosystems which have been as yet unexplored. MDPI 2022-03-23 /pmc/articles/PMC9028785/ /pubmed/35454078 http://dx.doi.org/10.3390/biom12040489 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pal, Yash
Mayilraj, Shanmugam
Krishnamurthi, Srinivasan
Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
title Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
title_full Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
title_fullStr Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
title_full_unstemmed Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
title_short Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
title_sort exploring the distinct distribution of archaeal communities in sites contaminated with explosives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028785/
https://www.ncbi.nlm.nih.gov/pubmed/35454078
http://dx.doi.org/10.3390/biom12040489
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