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Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering

The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtain...

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Autores principales: Blanco-Elices, Cristina, Chato-Astrain, Jesús, González-González, Alberto, Sánchez-Porras, David, Carriel, Víctor, Fernández-Valadés, Ricardo, Sánchez-Quevedo, María del Carmen, Alaminos, Miguel, Garzón, Ingrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028794/
https://www.ncbi.nlm.nih.gov/pubmed/35455764
http://dx.doi.org/10.3390/jpm12040648
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author Blanco-Elices, Cristina
Chato-Astrain, Jesús
González-González, Alberto
Sánchez-Porras, David
Carriel, Víctor
Fernández-Valadés, Ricardo
Sánchez-Quevedo, María del Carmen
Alaminos, Miguel
Garzón, Ingrid
author_facet Blanco-Elices, Cristina
Chato-Astrain, Jesús
González-González, Alberto
Sánchez-Porras, David
Carriel, Víctor
Fernández-Valadés, Ricardo
Sánchez-Quevedo, María del Carmen
Alaminos, Miguel
Garzón, Ingrid
author_sort Blanco-Elices, Cristina
collection PubMed
description The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtained and histologically prepared for in the situ analysis of four hUC regions (intervascular—IV, perivascular—PV, subaminoblastic—SAM, and Wharton’s jelly—WH), and primary cell cultures of mesenchymal stem cells (hUC-MSC) isolated from each region were obtained. The results confirmed the heterogeneity of the hUC, with the IV and PV zones tending to show the higher in situ expression of several components of the extracellular matrix (collagens, proteoglycans, and glycosaminoglycans), vimentin, and MSC markers (especially CD73), although isolation and ex vivo culture resulted in a homogeneous cell profile. Three vascular markers were positive in situ, especially vWF, followed by CD34 and CD31, and isolation and culture revealed that the region associated with the highest expression of vascular markers was IV, followed by PV. These results confirm the heterogeneity of the hUC and the need for selecting cells from specific regions of the hUC for particular applications in tissue engineering.
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spelling pubmed-90287942022-04-23 Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering Blanco-Elices, Cristina Chato-Astrain, Jesús González-González, Alberto Sánchez-Porras, David Carriel, Víctor Fernández-Valadés, Ricardo Sánchez-Quevedo, María del Carmen Alaminos, Miguel Garzón, Ingrid J Pers Med Article The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtained and histologically prepared for in the situ analysis of four hUC regions (intervascular—IV, perivascular—PV, subaminoblastic—SAM, and Wharton’s jelly—WH), and primary cell cultures of mesenchymal stem cells (hUC-MSC) isolated from each region were obtained. The results confirmed the heterogeneity of the hUC, with the IV and PV zones tending to show the higher in situ expression of several components of the extracellular matrix (collagens, proteoglycans, and glycosaminoglycans), vimentin, and MSC markers (especially CD73), although isolation and ex vivo culture resulted in a homogeneous cell profile. Three vascular markers were positive in situ, especially vWF, followed by CD34 and CD31, and isolation and culture revealed that the region associated with the highest expression of vascular markers was IV, followed by PV. These results confirm the heterogeneity of the hUC and the need for selecting cells from specific regions of the hUC for particular applications in tissue engineering. MDPI 2022-04-18 /pmc/articles/PMC9028794/ /pubmed/35455764 http://dx.doi.org/10.3390/jpm12040648 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Blanco-Elices, Cristina
Chato-Astrain, Jesús
González-González, Alberto
Sánchez-Porras, David
Carriel, Víctor
Fernández-Valadés, Ricardo
Sánchez-Quevedo, María del Carmen
Alaminos, Miguel
Garzón, Ingrid
Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
title Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
title_full Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
title_fullStr Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
title_full_unstemmed Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
title_short Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
title_sort histological profiling of the human umbilical cord: a potential alternative cell source in tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028794/
https://www.ncbi.nlm.nih.gov/pubmed/35455764
http://dx.doi.org/10.3390/jpm12040648
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