Cargando…
Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Bef...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029064/ https://www.ncbi.nlm.nih.gov/pubmed/35447943 http://dx.doi.org/10.3390/md20040270 |
_version_ | 1784691783865729024 |
---|---|
author | Papin, Marion Guimaraes, Cyrille Pierre-Aue, Benoit Fontaine, Delphine Pardessus, Jeoffrey Couthon, Hélène Fromont, Gaëlle Mahéo, Karine Chantôme, Aurélie Vandier, Christophe Pinault, Michelle |
author_facet | Papin, Marion Guimaraes, Cyrille Pierre-Aue, Benoit Fontaine, Delphine Pardessus, Jeoffrey Couthon, Hélène Fromont, Gaëlle Mahéo, Karine Chantôme, Aurélie Vandier, Christophe Pinault, Michelle |
author_sort | Papin, Marion |
collection | PubMed |
description | Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Before studying the biological role of molecular species of ether glycerolipids, there is a need to separate and quantify total alkyl and alkenyl glycerolipids from biological samples in order to determine any variation depending on tissue or physiopathological conditions. Here, we detail the development of the first high-performance thin-layer chromatography method for the quantification of total alkyl and alkenyl glycerolipids thanks to the separation of their corresponding alkyl and alkenyl glycerols. This method starts with a reduction of all lipids after extraction, resulting in the reduction of neutral and polar ether glycerolipids into alkyl and alkenyl glycerols, followed by an appropriate purification and, finally, the linearly ascending development of alkyl and alkenyl glycerols on high-performance thin-layer chromatography plates, staining, carbonization and densitometric analysis. Calibration curves were obtained with commercial alkyl and alkenyl glycerol standards, enabling the quantification of alkyl and alkenyl glycerols in samples and thus directly obtaining the quantity of alkyl and alkenyl lipids present in the samples. Interestingly, we found a differential quantity of these lipids in shark liver oil compared to chimera. We quantified alkyl and alkenyl glycerolipids in periprostatic adipose tissues from human prostate cancer and showed the feasibility of this method in other biological matrices (muscle, tumor). |
format | Online Article Text |
id | pubmed-9029064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90290642022-04-23 Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues Papin, Marion Guimaraes, Cyrille Pierre-Aue, Benoit Fontaine, Delphine Pardessus, Jeoffrey Couthon, Hélène Fromont, Gaëlle Mahéo, Karine Chantôme, Aurélie Vandier, Christophe Pinault, Michelle Mar Drugs Article Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Before studying the biological role of molecular species of ether glycerolipids, there is a need to separate and quantify total alkyl and alkenyl glycerolipids from biological samples in order to determine any variation depending on tissue or physiopathological conditions. Here, we detail the development of the first high-performance thin-layer chromatography method for the quantification of total alkyl and alkenyl glycerolipids thanks to the separation of their corresponding alkyl and alkenyl glycerols. This method starts with a reduction of all lipids after extraction, resulting in the reduction of neutral and polar ether glycerolipids into alkyl and alkenyl glycerols, followed by an appropriate purification and, finally, the linearly ascending development of alkyl and alkenyl glycerols on high-performance thin-layer chromatography plates, staining, carbonization and densitometric analysis. Calibration curves were obtained with commercial alkyl and alkenyl glycerol standards, enabling the quantification of alkyl and alkenyl glycerols in samples and thus directly obtaining the quantity of alkyl and alkenyl lipids present in the samples. Interestingly, we found a differential quantity of these lipids in shark liver oil compared to chimera. We quantified alkyl and alkenyl glycerolipids in periprostatic adipose tissues from human prostate cancer and showed the feasibility of this method in other biological matrices (muscle, tumor). MDPI 2022-04-18 /pmc/articles/PMC9029064/ /pubmed/35447943 http://dx.doi.org/10.3390/md20040270 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Papin, Marion Guimaraes, Cyrille Pierre-Aue, Benoit Fontaine, Delphine Pardessus, Jeoffrey Couthon, Hélène Fromont, Gaëlle Mahéo, Karine Chantôme, Aurélie Vandier, Christophe Pinault, Michelle Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues |
title | Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues |
title_full | Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues |
title_fullStr | Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues |
title_full_unstemmed | Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues |
title_short | Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues |
title_sort | development of a high-performance thin-layer chromatography method for the quantification of alkyl glycerolipids and alkenyl glycerolipids from shark and chimera oils and tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029064/ https://www.ncbi.nlm.nih.gov/pubmed/35447943 http://dx.doi.org/10.3390/md20040270 |
work_keys_str_mv | AT papinmarion developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT guimaraescyrille developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT pierreauebenoit developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT fontainedelphine developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT pardessusjeoffrey developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT couthonhelene developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT fromontgaelle developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT maheokarine developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT chantomeaurelie developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT vandierchristophe developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues AT pinaultmichelle developmentofahighperformancethinlayerchromatographymethodforthequantificationofalkylglycerolipidsandalkenylglycerolipidsfromsharkandchimeraoilsandtissues |