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Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues

Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Bef...

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Autores principales: Papin, Marion, Guimaraes, Cyrille, Pierre-Aue, Benoit, Fontaine, Delphine, Pardessus, Jeoffrey, Couthon, Hélène, Fromont, Gaëlle, Mahéo, Karine, Chantôme, Aurélie, Vandier, Christophe, Pinault, Michelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029064/
https://www.ncbi.nlm.nih.gov/pubmed/35447943
http://dx.doi.org/10.3390/md20040270
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author Papin, Marion
Guimaraes, Cyrille
Pierre-Aue, Benoit
Fontaine, Delphine
Pardessus, Jeoffrey
Couthon, Hélène
Fromont, Gaëlle
Mahéo, Karine
Chantôme, Aurélie
Vandier, Christophe
Pinault, Michelle
author_facet Papin, Marion
Guimaraes, Cyrille
Pierre-Aue, Benoit
Fontaine, Delphine
Pardessus, Jeoffrey
Couthon, Hélène
Fromont, Gaëlle
Mahéo, Karine
Chantôme, Aurélie
Vandier, Christophe
Pinault, Michelle
author_sort Papin, Marion
collection PubMed
description Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Before studying the biological role of molecular species of ether glycerolipids, there is a need to separate and quantify total alkyl and alkenyl glycerolipids from biological samples in order to determine any variation depending on tissue or physiopathological conditions. Here, we detail the development of the first high-performance thin-layer chromatography method for the quantification of total alkyl and alkenyl glycerolipids thanks to the separation of their corresponding alkyl and alkenyl glycerols. This method starts with a reduction of all lipids after extraction, resulting in the reduction of neutral and polar ether glycerolipids into alkyl and alkenyl glycerols, followed by an appropriate purification and, finally, the linearly ascending development of alkyl and alkenyl glycerols on high-performance thin-layer chromatography plates, staining, carbonization and densitometric analysis. Calibration curves were obtained with commercial alkyl and alkenyl glycerol standards, enabling the quantification of alkyl and alkenyl glycerols in samples and thus directly obtaining the quantity of alkyl and alkenyl lipids present in the samples. Interestingly, we found a differential quantity of these lipids in shark liver oil compared to chimera. We quantified alkyl and alkenyl glycerolipids in periprostatic adipose tissues from human prostate cancer and showed the feasibility of this method in other biological matrices (muscle, tumor).
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spelling pubmed-90290642022-04-23 Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues Papin, Marion Guimaraes, Cyrille Pierre-Aue, Benoit Fontaine, Delphine Pardessus, Jeoffrey Couthon, Hélène Fromont, Gaëlle Mahéo, Karine Chantôme, Aurélie Vandier, Christophe Pinault, Michelle Mar Drugs Article Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Before studying the biological role of molecular species of ether glycerolipids, there is a need to separate and quantify total alkyl and alkenyl glycerolipids from biological samples in order to determine any variation depending on tissue or physiopathological conditions. Here, we detail the development of the first high-performance thin-layer chromatography method for the quantification of total alkyl and alkenyl glycerolipids thanks to the separation of their corresponding alkyl and alkenyl glycerols. This method starts with a reduction of all lipids after extraction, resulting in the reduction of neutral and polar ether glycerolipids into alkyl and alkenyl glycerols, followed by an appropriate purification and, finally, the linearly ascending development of alkyl and alkenyl glycerols on high-performance thin-layer chromatography plates, staining, carbonization and densitometric analysis. Calibration curves were obtained with commercial alkyl and alkenyl glycerol standards, enabling the quantification of alkyl and alkenyl glycerols in samples and thus directly obtaining the quantity of alkyl and alkenyl lipids present in the samples. Interestingly, we found a differential quantity of these lipids in shark liver oil compared to chimera. We quantified alkyl and alkenyl glycerolipids in periprostatic adipose tissues from human prostate cancer and showed the feasibility of this method in other biological matrices (muscle, tumor). MDPI 2022-04-18 /pmc/articles/PMC9029064/ /pubmed/35447943 http://dx.doi.org/10.3390/md20040270 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Papin, Marion
Guimaraes, Cyrille
Pierre-Aue, Benoit
Fontaine, Delphine
Pardessus, Jeoffrey
Couthon, Hélène
Fromont, Gaëlle
Mahéo, Karine
Chantôme, Aurélie
Vandier, Christophe
Pinault, Michelle
Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
title Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
title_full Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
title_fullStr Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
title_full_unstemmed Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
title_short Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
title_sort development of a high-performance thin-layer chromatography method for the quantification of alkyl glycerolipids and alkenyl glycerolipids from shark and chimera oils and tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029064/
https://www.ncbi.nlm.nih.gov/pubmed/35447943
http://dx.doi.org/10.3390/md20040270
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