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Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand

Dirty panicle disease in coconuts (Cocos nucifera) was first observed in the KU-BEDO Coconut BioBank, Nakhon Pathom province, Thailand. The occurrence of the disease covers more than 30% of the total coconut plantation area. The symptoms include small brown to dark brown spots and discoloration of m...

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Autores principales: Sunpapao, Anurag, Suwannarach, Nakarin, Kumla, Jaturong, Dumhai, Reajina, Riangwong, Kanamon, Sanguansub, Sunisa, Wanchana, Samart, Arikit, Siwaret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029170/
https://www.ncbi.nlm.nih.gov/pubmed/35448566
http://dx.doi.org/10.3390/jof8040335
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author Sunpapao, Anurag
Suwannarach, Nakarin
Kumla, Jaturong
Dumhai, Reajina
Riangwong, Kanamon
Sanguansub, Sunisa
Wanchana, Samart
Arikit, Siwaret
author_facet Sunpapao, Anurag
Suwannarach, Nakarin
Kumla, Jaturong
Dumhai, Reajina
Riangwong, Kanamon
Sanguansub, Sunisa
Wanchana, Samart
Arikit, Siwaret
author_sort Sunpapao, Anurag
collection PubMed
description Dirty panicle disease in coconuts (Cocos nucifera) was first observed in the KU-BEDO Coconut BioBank, Nakhon Pathom province, Thailand. The occurrence of the disease covers more than 30% of the total coconut plantation area. The symptoms include small brown to dark brown spots and discoloration of male flowers. Herein, three fungal strains were isolated from infected samples. Based on the morphological characteristics the fungal isolates, they were classified into two genera, namely, Alternaria (Al01) and Fusarium (FUO01 and FUP01). DNA sequences of internal transcribed spacer (ITS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), translation elongation factor 1-α (tef1-α), and RNA polymerase II second largest subunit (rpb2) revealed Al01 as Alternaria burnsii, whereas DNA sequences of ITS, rpb2, and tef1-α identified FUO01 and FUP01 as Fusarium clavum and F. tricinctum, respectively. A pathogenicity test by the agar plug method demonstrated that these pathogens cause dirty panicle disease similar to that observed in natural infections. To the best of our knowledge, this is the first report on the novel dirty panicle disease in coconuts in Thailand or elsewhere, demonstrating that it is associated with the plant pathogenic fungi A. burnsii, F. clavum, and F. tricinctum.
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spelling pubmed-90291702022-04-23 Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand Sunpapao, Anurag Suwannarach, Nakarin Kumla, Jaturong Dumhai, Reajina Riangwong, Kanamon Sanguansub, Sunisa Wanchana, Samart Arikit, Siwaret J Fungi (Basel) Article Dirty panicle disease in coconuts (Cocos nucifera) was first observed in the KU-BEDO Coconut BioBank, Nakhon Pathom province, Thailand. The occurrence of the disease covers more than 30% of the total coconut plantation area. The symptoms include small brown to dark brown spots and discoloration of male flowers. Herein, three fungal strains were isolated from infected samples. Based on the morphological characteristics the fungal isolates, they were classified into two genera, namely, Alternaria (Al01) and Fusarium (FUO01 and FUP01). DNA sequences of internal transcribed spacer (ITS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), translation elongation factor 1-α (tef1-α), and RNA polymerase II second largest subunit (rpb2) revealed Al01 as Alternaria burnsii, whereas DNA sequences of ITS, rpb2, and tef1-α identified FUO01 and FUP01 as Fusarium clavum and F. tricinctum, respectively. A pathogenicity test by the agar plug method demonstrated that these pathogens cause dirty panicle disease similar to that observed in natural infections. To the best of our knowledge, this is the first report on the novel dirty panicle disease in coconuts in Thailand or elsewhere, demonstrating that it is associated with the plant pathogenic fungi A. burnsii, F. clavum, and F. tricinctum. MDPI 2022-03-23 /pmc/articles/PMC9029170/ /pubmed/35448566 http://dx.doi.org/10.3390/jof8040335 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sunpapao, Anurag
Suwannarach, Nakarin
Kumla, Jaturong
Dumhai, Reajina
Riangwong, Kanamon
Sanguansub, Sunisa
Wanchana, Samart
Arikit, Siwaret
Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
title Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
title_full Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
title_fullStr Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
title_full_unstemmed Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
title_short Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
title_sort morphological and molecular identification of plant pathogenic fungi associated with dirty panicle disease in coconuts (cocos nucifera) in thailand
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029170/
https://www.ncbi.nlm.nih.gov/pubmed/35448566
http://dx.doi.org/10.3390/jof8040335
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