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Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films

Chitosan-based film with and without antagonistic yeast was prepared and its effect against Penicillium italicum was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical properties of the prepar...

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Autores principales: García-Bramasco, César A., Blancas-Benitez, Francisco J., Montaño-Leyva, Beatriz, Medrano-Castellón, Laura M., Gutierrez-Martinez, Porfirio, González-Estrada, Ramsés R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029259/
https://www.ncbi.nlm.nih.gov/pubmed/35448600
http://dx.doi.org/10.3390/jof8040369
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author García-Bramasco, César A.
Blancas-Benitez, Francisco J.
Montaño-Leyva, Beatriz
Medrano-Castellón, Laura M.
Gutierrez-Martinez, Porfirio
González-Estrada, Ramsés R.
author_facet García-Bramasco, César A.
Blancas-Benitez, Francisco J.
Montaño-Leyva, Beatriz
Medrano-Castellón, Laura M.
Gutierrez-Martinez, Porfirio
González-Estrada, Ramsés R.
author_sort García-Bramasco, César A.
collection PubMed
description Chitosan-based film with and without antagonistic yeast was prepared and its effect against Penicillium italicum was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical properties of the prepared films with and without yeast cells incorporated were evaluated in terms of thickness, mechanical properties, color and opacity. Chitosan films with the antagonistic yeast entrapped exhibited strong antifungal activity by inhibiting the mycelial development (55%), germination (45%) and reducing the sporulation process (87%). Chitosan matrix at 0.5% and 1.0% was maintained over 9 days of cell viability. However, at 1.5% of chitosan the population dynamics was strongly affected. The addition of yeast cells only impacted color values such as a*, b*, chroma and hue angle when 1.0% of chitosan concentration was used. Conversely, luminosity was not affected in the presence of yeast cells as well as the opacity. Besides, the addition of antagonistic yeast improved the mechanical resistance of the films. The addition of D. hansenii in chitosan films improve their efficacy for controlling P. italicum, and besides showed desirable characteristics for future use as packaging for citrus products.
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spelling pubmed-90292592022-04-23 Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films García-Bramasco, César A. Blancas-Benitez, Francisco J. Montaño-Leyva, Beatriz Medrano-Castellón, Laura M. Gutierrez-Martinez, Porfirio González-Estrada, Ramsés R. J Fungi (Basel) Article Chitosan-based film with and without antagonistic yeast was prepared and its effect against Penicillium italicum was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical properties of the prepared films with and without yeast cells incorporated were evaluated in terms of thickness, mechanical properties, color and opacity. Chitosan films with the antagonistic yeast entrapped exhibited strong antifungal activity by inhibiting the mycelial development (55%), germination (45%) and reducing the sporulation process (87%). Chitosan matrix at 0.5% and 1.0% was maintained over 9 days of cell viability. However, at 1.5% of chitosan the population dynamics was strongly affected. The addition of yeast cells only impacted color values such as a*, b*, chroma and hue angle when 1.0% of chitosan concentration was used. Conversely, luminosity was not affected in the presence of yeast cells as well as the opacity. Besides, the addition of antagonistic yeast improved the mechanical resistance of the films. The addition of D. hansenii in chitosan films improve their efficacy for controlling P. italicum, and besides showed desirable characteristics for future use as packaging for citrus products. MDPI 2022-04-04 /pmc/articles/PMC9029259/ /pubmed/35448600 http://dx.doi.org/10.3390/jof8040369 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Bramasco, César A.
Blancas-Benitez, Francisco J.
Montaño-Leyva, Beatriz
Medrano-Castellón, Laura M.
Gutierrez-Martinez, Porfirio
González-Estrada, Ramsés R.
Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
title Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
title_full Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
title_fullStr Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
title_full_unstemmed Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
title_short Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
title_sort influence of marine yeast debaryomyces hansenii on antifungal and physicochemical properties of chitosan-based films
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029259/
https://www.ncbi.nlm.nih.gov/pubmed/35448600
http://dx.doi.org/10.3390/jof8040369
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