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Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model

Through a multitude of studies, the gut microbiota has been recognized as a significant influencer of both homeostasis and pathophysiology. Certain microbial taxa can even affect treatments such as cancer immunotherapies, including the immune checkpoint blockade. These taxa can impact such processes...

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Autores principales: Rossi, Marco, Aspromonte, Salvatore M., Kohlhapp, Frederick J., Newman, Jenna H., Lemenze, Alex, Pepe, Russell J., DeFina, Samuel M., Herzog, Nora L., Donnelly, Robert, Kuzel, Timothy M., Reiser, Jochen, Guevara-Patino, Jose A., Zloza, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029337/
https://www.ncbi.nlm.nih.gov/pubmed/35454006
http://dx.doi.org/10.3390/diagnostics12040958
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author Rossi, Marco
Aspromonte, Salvatore M.
Kohlhapp, Frederick J.
Newman, Jenna H.
Lemenze, Alex
Pepe, Russell J.
DeFina, Samuel M.
Herzog, Nora L.
Donnelly, Robert
Kuzel, Timothy M.
Reiser, Jochen
Guevara-Patino, Jose A.
Zloza, Andrew
author_facet Rossi, Marco
Aspromonte, Salvatore M.
Kohlhapp, Frederick J.
Newman, Jenna H.
Lemenze, Alex
Pepe, Russell J.
DeFina, Samuel M.
Herzog, Nora L.
Donnelly, Robert
Kuzel, Timothy M.
Reiser, Jochen
Guevara-Patino, Jose A.
Zloza, Andrew
author_sort Rossi, Marco
collection PubMed
description Through a multitude of studies, the gut microbiota has been recognized as a significant influencer of both homeostasis and pathophysiology. Certain microbial taxa can even affect treatments such as cancer immunotherapies, including the immune checkpoint blockade. These taxa can impact such processes both individually as well as collectively through mechanisms from quorum sensing to metabolite production. Due to this overarching presence of the gut microbiota in many physiological processes distal to the GI tract, we hypothesized that mice bearing tumors at extraintestinal sites would display a distinct intestinal microbial signature from non-tumor-bearing mice, and that such a signature would involve taxa that collectively shift with tumor presence. Microbial OTUs were determined from 16S rRNA genes isolated from the fecal samples of C57BL/6 mice challenged with either B16-F10 melanoma cells or PBS control and analyzed using QIIME. Relative proportions of bacteria were determined for each mouse and, using machine-learning approaches, significantly altered taxa and co-occurrence patterns between tumor- and non-tumor-bearing mice were found. Mice with a tumor had elevated proportions of Ruminococcaceae, Peptococcaceae.g_rc4.4, and Christensenellaceae, as well as significant information gains and ReliefF weights for Bacteroidales.f__S24.7, Ruminococcaceae, Clostridiales, and Erysipelotrichaceae. Bacteroidales.f__S24.7, Ruminococcaceae, and Clostridiales were also implicated through shifting co-occurrences and PCA values. Using these seven taxa as a melanoma signature, a neural network reached an 80% tumor detection accuracy in a 10-fold stratified random sampling validation. These results indicated gut microbial proportions as a biosensor for tumor detection, and that shifting co-occurrences could be used to reveal relevant taxa.
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spelling pubmed-90293372022-04-23 Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model Rossi, Marco Aspromonte, Salvatore M. Kohlhapp, Frederick J. Newman, Jenna H. Lemenze, Alex Pepe, Russell J. DeFina, Samuel M. Herzog, Nora L. Donnelly, Robert Kuzel, Timothy M. Reiser, Jochen Guevara-Patino, Jose A. Zloza, Andrew Diagnostics (Basel) Article Through a multitude of studies, the gut microbiota has been recognized as a significant influencer of both homeostasis and pathophysiology. Certain microbial taxa can even affect treatments such as cancer immunotherapies, including the immune checkpoint blockade. These taxa can impact such processes both individually as well as collectively through mechanisms from quorum sensing to metabolite production. Due to this overarching presence of the gut microbiota in many physiological processes distal to the GI tract, we hypothesized that mice bearing tumors at extraintestinal sites would display a distinct intestinal microbial signature from non-tumor-bearing mice, and that such a signature would involve taxa that collectively shift with tumor presence. Microbial OTUs were determined from 16S rRNA genes isolated from the fecal samples of C57BL/6 mice challenged with either B16-F10 melanoma cells or PBS control and analyzed using QIIME. Relative proportions of bacteria were determined for each mouse and, using machine-learning approaches, significantly altered taxa and co-occurrence patterns between tumor- and non-tumor-bearing mice were found. Mice with a tumor had elevated proportions of Ruminococcaceae, Peptococcaceae.g_rc4.4, and Christensenellaceae, as well as significant information gains and ReliefF weights for Bacteroidales.f__S24.7, Ruminococcaceae, Clostridiales, and Erysipelotrichaceae. Bacteroidales.f__S24.7, Ruminococcaceae, and Clostridiales were also implicated through shifting co-occurrences and PCA values. Using these seven taxa as a melanoma signature, a neural network reached an 80% tumor detection accuracy in a 10-fold stratified random sampling validation. These results indicated gut microbial proportions as a biosensor for tumor detection, and that shifting co-occurrences could be used to reveal relevant taxa. MDPI 2022-04-12 /pmc/articles/PMC9029337/ /pubmed/35454006 http://dx.doi.org/10.3390/diagnostics12040958 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rossi, Marco
Aspromonte, Salvatore M.
Kohlhapp, Frederick J.
Newman, Jenna H.
Lemenze, Alex
Pepe, Russell J.
DeFina, Samuel M.
Herzog, Nora L.
Donnelly, Robert
Kuzel, Timothy M.
Reiser, Jochen
Guevara-Patino, Jose A.
Zloza, Andrew
Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
title Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
title_full Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
title_fullStr Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
title_full_unstemmed Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
title_short Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
title_sort gut microbial shifts indicate melanoma presence and bacterial interactions in a murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029337/
https://www.ncbi.nlm.nih.gov/pubmed/35454006
http://dx.doi.org/10.3390/diagnostics12040958
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