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Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model
Through a multitude of studies, the gut microbiota has been recognized as a significant influencer of both homeostasis and pathophysiology. Certain microbial taxa can even affect treatments such as cancer immunotherapies, including the immune checkpoint blockade. These taxa can impact such processes...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029337/ https://www.ncbi.nlm.nih.gov/pubmed/35454006 http://dx.doi.org/10.3390/diagnostics12040958 |
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author | Rossi, Marco Aspromonte, Salvatore M. Kohlhapp, Frederick J. Newman, Jenna H. Lemenze, Alex Pepe, Russell J. DeFina, Samuel M. Herzog, Nora L. Donnelly, Robert Kuzel, Timothy M. Reiser, Jochen Guevara-Patino, Jose A. Zloza, Andrew |
author_facet | Rossi, Marco Aspromonte, Salvatore M. Kohlhapp, Frederick J. Newman, Jenna H. Lemenze, Alex Pepe, Russell J. DeFina, Samuel M. Herzog, Nora L. Donnelly, Robert Kuzel, Timothy M. Reiser, Jochen Guevara-Patino, Jose A. Zloza, Andrew |
author_sort | Rossi, Marco |
collection | PubMed |
description | Through a multitude of studies, the gut microbiota has been recognized as a significant influencer of both homeostasis and pathophysiology. Certain microbial taxa can even affect treatments such as cancer immunotherapies, including the immune checkpoint blockade. These taxa can impact such processes both individually as well as collectively through mechanisms from quorum sensing to metabolite production. Due to this overarching presence of the gut microbiota in many physiological processes distal to the GI tract, we hypothesized that mice bearing tumors at extraintestinal sites would display a distinct intestinal microbial signature from non-tumor-bearing mice, and that such a signature would involve taxa that collectively shift with tumor presence. Microbial OTUs were determined from 16S rRNA genes isolated from the fecal samples of C57BL/6 mice challenged with either B16-F10 melanoma cells or PBS control and analyzed using QIIME. Relative proportions of bacteria were determined for each mouse and, using machine-learning approaches, significantly altered taxa and co-occurrence patterns between tumor- and non-tumor-bearing mice were found. Mice with a tumor had elevated proportions of Ruminococcaceae, Peptococcaceae.g_rc4.4, and Christensenellaceae, as well as significant information gains and ReliefF weights for Bacteroidales.f__S24.7, Ruminococcaceae, Clostridiales, and Erysipelotrichaceae. Bacteroidales.f__S24.7, Ruminococcaceae, and Clostridiales were also implicated through shifting co-occurrences and PCA values. Using these seven taxa as a melanoma signature, a neural network reached an 80% tumor detection accuracy in a 10-fold stratified random sampling validation. These results indicated gut microbial proportions as a biosensor for tumor detection, and that shifting co-occurrences could be used to reveal relevant taxa. |
format | Online Article Text |
id | pubmed-9029337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90293372022-04-23 Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model Rossi, Marco Aspromonte, Salvatore M. Kohlhapp, Frederick J. Newman, Jenna H. Lemenze, Alex Pepe, Russell J. DeFina, Samuel M. Herzog, Nora L. Donnelly, Robert Kuzel, Timothy M. Reiser, Jochen Guevara-Patino, Jose A. Zloza, Andrew Diagnostics (Basel) Article Through a multitude of studies, the gut microbiota has been recognized as a significant influencer of both homeostasis and pathophysiology. Certain microbial taxa can even affect treatments such as cancer immunotherapies, including the immune checkpoint blockade. These taxa can impact such processes both individually as well as collectively through mechanisms from quorum sensing to metabolite production. Due to this overarching presence of the gut microbiota in many physiological processes distal to the GI tract, we hypothesized that mice bearing tumors at extraintestinal sites would display a distinct intestinal microbial signature from non-tumor-bearing mice, and that such a signature would involve taxa that collectively shift with tumor presence. Microbial OTUs were determined from 16S rRNA genes isolated from the fecal samples of C57BL/6 mice challenged with either B16-F10 melanoma cells or PBS control and analyzed using QIIME. Relative proportions of bacteria were determined for each mouse and, using machine-learning approaches, significantly altered taxa and co-occurrence patterns between tumor- and non-tumor-bearing mice were found. Mice with a tumor had elevated proportions of Ruminococcaceae, Peptococcaceae.g_rc4.4, and Christensenellaceae, as well as significant information gains and ReliefF weights for Bacteroidales.f__S24.7, Ruminococcaceae, Clostridiales, and Erysipelotrichaceae. Bacteroidales.f__S24.7, Ruminococcaceae, and Clostridiales were also implicated through shifting co-occurrences and PCA values. Using these seven taxa as a melanoma signature, a neural network reached an 80% tumor detection accuracy in a 10-fold stratified random sampling validation. These results indicated gut microbial proportions as a biosensor for tumor detection, and that shifting co-occurrences could be used to reveal relevant taxa. MDPI 2022-04-12 /pmc/articles/PMC9029337/ /pubmed/35454006 http://dx.doi.org/10.3390/diagnostics12040958 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rossi, Marco Aspromonte, Salvatore M. Kohlhapp, Frederick J. Newman, Jenna H. Lemenze, Alex Pepe, Russell J. DeFina, Samuel M. Herzog, Nora L. Donnelly, Robert Kuzel, Timothy M. Reiser, Jochen Guevara-Patino, Jose A. Zloza, Andrew Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model |
title | Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model |
title_full | Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model |
title_fullStr | Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model |
title_full_unstemmed | Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model |
title_short | Gut Microbial Shifts Indicate Melanoma Presence and Bacterial Interactions in a Murine Model |
title_sort | gut microbial shifts indicate melanoma presence and bacterial interactions in a murine model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029337/ https://www.ncbi.nlm.nih.gov/pubmed/35454006 http://dx.doi.org/10.3390/diagnostics12040958 |
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