Cargando…

No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020

Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed...

Descripción completa

Detalles Bibliográficos
Autores principales: Faber, Mirko, Wenzel, Jürgen J., Erl, Monika, Stark, Klaus, Schemmerer, Mathias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029421/
https://www.ncbi.nlm.nih.gov/pubmed/35458471
http://dx.doi.org/10.3390/v14040742
_version_ 1784691873721352192
author Faber, Mirko
Wenzel, Jürgen J.
Erl, Monika
Stark, Klaus
Schemmerer, Mathias
author_facet Faber, Mirko
Wenzel, Jürgen J.
Erl, Monika
Stark, Klaus
Schemmerer, Mathias
author_sort Faber, Mirko
collection PubMed
description Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed or misclassified as conventional HEV (Orthohepevirus A) infections. We conducted a retrospective screening study among serum and plasma samples from patients suspected of having HEV infection, which were archived at the national consultant laboratory for HAV and HEV between 2000 and 2020. We randomly selected n = 200 samples, which were initially tested reactive (positive or borderline) for HEV-IgM and negative for HEV RNA and re-examined them using a highly sensitive Orthohepevirus C genotype 1-specific in-house RT-qPCR (LoD 95: 6.73 copies per reaction) and a nested RT-PCR broadly reactive for Orthohepevirus A and C. Conventional sanger sequencing was conducted for resulting PCR products. No atypical HEV strains were detected (0 of 200 [0.0%; 95% confidence interval: 0.0%–1.89%], indicating that Orthohepevirus C infections in the investigated population (persons with clinical suspicion of hepatitis E and positive HEV-IgM) are very rare.
format Online
Article
Text
id pubmed-9029421
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90294212022-04-23 No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 Faber, Mirko Wenzel, Jürgen J. Erl, Monika Stark, Klaus Schemmerer, Mathias Viruses Article Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed or misclassified as conventional HEV (Orthohepevirus A) infections. We conducted a retrospective screening study among serum and plasma samples from patients suspected of having HEV infection, which were archived at the national consultant laboratory for HAV and HEV between 2000 and 2020. We randomly selected n = 200 samples, which were initially tested reactive (positive or borderline) for HEV-IgM and negative for HEV RNA and re-examined them using a highly sensitive Orthohepevirus C genotype 1-specific in-house RT-qPCR (LoD 95: 6.73 copies per reaction) and a nested RT-PCR broadly reactive for Orthohepevirus A and C. Conventional sanger sequencing was conducted for resulting PCR products. No atypical HEV strains were detected (0 of 200 [0.0%; 95% confidence interval: 0.0%–1.89%], indicating that Orthohepevirus C infections in the investigated population (persons with clinical suspicion of hepatitis E and positive HEV-IgM) are very rare. MDPI 2022-03-31 /pmc/articles/PMC9029421/ /pubmed/35458471 http://dx.doi.org/10.3390/v14040742 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Faber, Mirko
Wenzel, Jürgen J.
Erl, Monika
Stark, Klaus
Schemmerer, Mathias
No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
title No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
title_full No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
title_fullStr No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
title_full_unstemmed No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
title_short No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
title_sort no evidence for orthohepevirus c in archived human samples in germany, 2000–2020
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029421/
https://www.ncbi.nlm.nih.gov/pubmed/35458471
http://dx.doi.org/10.3390/v14040742
work_keys_str_mv AT fabermirko noevidencefororthohepeviruscinarchivedhumansamplesingermany20002020
AT wenzeljurgenj noevidencefororthohepeviruscinarchivedhumansamplesingermany20002020
AT erlmonika noevidencefororthohepeviruscinarchivedhumansamplesingermany20002020
AT starkklaus noevidencefororthohepeviruscinarchivedhumansamplesingermany20002020
AT schemmerermathias noevidencefororthohepeviruscinarchivedhumansamplesingermany20002020