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No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029421/ https://www.ncbi.nlm.nih.gov/pubmed/35458471 http://dx.doi.org/10.3390/v14040742 |
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author | Faber, Mirko Wenzel, Jürgen J. Erl, Monika Stark, Klaus Schemmerer, Mathias |
author_facet | Faber, Mirko Wenzel, Jürgen J. Erl, Monika Stark, Klaus Schemmerer, Mathias |
author_sort | Faber, Mirko |
collection | PubMed |
description | Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed or misclassified as conventional HEV (Orthohepevirus A) infections. We conducted a retrospective screening study among serum and plasma samples from patients suspected of having HEV infection, which were archived at the national consultant laboratory for HAV and HEV between 2000 and 2020. We randomly selected n = 200 samples, which were initially tested reactive (positive or borderline) for HEV-IgM and negative for HEV RNA and re-examined them using a highly sensitive Orthohepevirus C genotype 1-specific in-house RT-qPCR (LoD 95: 6.73 copies per reaction) and a nested RT-PCR broadly reactive for Orthohepevirus A and C. Conventional sanger sequencing was conducted for resulting PCR products. No atypical HEV strains were detected (0 of 200 [0.0%; 95% confidence interval: 0.0%–1.89%], indicating that Orthohepevirus C infections in the investigated population (persons with clinical suspicion of hepatitis E and positive HEV-IgM) are very rare. |
format | Online Article Text |
id | pubmed-9029421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90294212022-04-23 No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 Faber, Mirko Wenzel, Jürgen J. Erl, Monika Stark, Klaus Schemmerer, Mathias Viruses Article Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed or misclassified as conventional HEV (Orthohepevirus A) infections. We conducted a retrospective screening study among serum and plasma samples from patients suspected of having HEV infection, which were archived at the national consultant laboratory for HAV and HEV between 2000 and 2020. We randomly selected n = 200 samples, which were initially tested reactive (positive or borderline) for HEV-IgM and negative for HEV RNA and re-examined them using a highly sensitive Orthohepevirus C genotype 1-specific in-house RT-qPCR (LoD 95: 6.73 copies per reaction) and a nested RT-PCR broadly reactive for Orthohepevirus A and C. Conventional sanger sequencing was conducted for resulting PCR products. No atypical HEV strains were detected (0 of 200 [0.0%; 95% confidence interval: 0.0%–1.89%], indicating that Orthohepevirus C infections in the investigated population (persons with clinical suspicion of hepatitis E and positive HEV-IgM) are very rare. MDPI 2022-03-31 /pmc/articles/PMC9029421/ /pubmed/35458471 http://dx.doi.org/10.3390/v14040742 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Faber, Mirko Wenzel, Jürgen J. Erl, Monika Stark, Klaus Schemmerer, Mathias No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 |
title | No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 |
title_full | No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 |
title_fullStr | No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 |
title_full_unstemmed | No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 |
title_short | No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020 |
title_sort | no evidence for orthohepevirus c in archived human samples in germany, 2000–2020 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029421/ https://www.ncbi.nlm.nih.gov/pubmed/35458471 http://dx.doi.org/10.3390/v14040742 |
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