Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope

The long-term storage stability of vaccines has a major impact on the roll-out and success of global immunization programs. For the Human Immunodeficiency Virus type 1 (HIV-1) virus-like particle (VLP) vaccine prototype evaluated here, nanoparticle tracking analysis (NTA), and enzyme-linked immunoab...

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Autores principales: Aguado-Garcia, Diana, Olvera, Alex, Brander, Christian, Sanchez-Merino, Victor, Yuste, Eloisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029469/
https://www.ncbi.nlm.nih.gov/pubmed/35455233
http://dx.doi.org/10.3390/vaccines10040484
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author Aguado-Garcia, Diana
Olvera, Alex
Brander, Christian
Sanchez-Merino, Victor
Yuste, Eloisa
author_facet Aguado-Garcia, Diana
Olvera, Alex
Brander, Christian
Sanchez-Merino, Victor
Yuste, Eloisa
author_sort Aguado-Garcia, Diana
collection PubMed
description The long-term storage stability of vaccines has a major impact on the roll-out and success of global immunization programs. For the Human Immunodeficiency Virus type 1 (HIV-1) virus-like particle (VLP) vaccine prototype evaluated here, nanoparticle tracking analysis (NTA), and enzyme-linked immunoabsorbent assay (ELISA) results demonstrated a remarkable structural stability. VLPs maintained their integrity and the recognition of relevant B-cell epitopes for three months at 4 and −20 °C. Interestingly, most particles remained intact and preserved the recognition of relevant epitopes even after a week of storage at room temperature.
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spelling pubmed-90294692022-04-23 Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope Aguado-Garcia, Diana Olvera, Alex Brander, Christian Sanchez-Merino, Victor Yuste, Eloisa Vaccines (Basel) Communication The long-term storage stability of vaccines has a major impact on the roll-out and success of global immunization programs. For the Human Immunodeficiency Virus type 1 (HIV-1) virus-like particle (VLP) vaccine prototype evaluated here, nanoparticle tracking analysis (NTA), and enzyme-linked immunoabsorbent assay (ELISA) results demonstrated a remarkable structural stability. VLPs maintained their integrity and the recognition of relevant B-cell epitopes for three months at 4 and −20 °C. Interestingly, most particles remained intact and preserved the recognition of relevant epitopes even after a week of storage at room temperature. MDPI 2022-03-22 /pmc/articles/PMC9029469/ /pubmed/35455233 http://dx.doi.org/10.3390/vaccines10040484 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Aguado-Garcia, Diana
Olvera, Alex
Brander, Christian
Sanchez-Merino, Victor
Yuste, Eloisa
Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope
title Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope
title_full Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope
title_fullStr Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope
title_full_unstemmed Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope
title_short Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope
title_sort evaluation of the thermal stability of a vaccine prototype based on virus-like particle formulated hiv-1 envelope
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029469/
https://www.ncbi.nlm.nih.gov/pubmed/35455233
http://dx.doi.org/10.3390/vaccines10040484
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