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Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions

Virus isolates are not only useful for diagnosing infections, e.g., respiratory syncytial virus (RSV), but can also facilitate many aspects of practical viral studies such as analyses of antigenicity and the action mechanisms of antivirals, among others. We have been isolating RSV from clinical spec...

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Autores principales: Kitai, Yuki, Sato, Ko, Shirato, Kazuya, Ohmiya, Suguru, Watanabe, Oshi, Kisu, Tomoko, Ota, Reiko, Takeda, Makoto, Kawakami, Kazuyoshi, Nishimura, Hidekazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029476/
https://www.ncbi.nlm.nih.gov/pubmed/35458409
http://dx.doi.org/10.3390/v14040679
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author Kitai, Yuki
Sato, Ko
Shirato, Kazuya
Ohmiya, Suguru
Watanabe, Oshi
Kisu, Tomoko
Ota, Reiko
Takeda, Makoto
Kawakami, Kazuyoshi
Nishimura, Hidekazu
author_facet Kitai, Yuki
Sato, Ko
Shirato, Kazuya
Ohmiya, Suguru
Watanabe, Oshi
Kisu, Tomoko
Ota, Reiko
Takeda, Makoto
Kawakami, Kazuyoshi
Nishimura, Hidekazu
author_sort Kitai, Yuki
collection PubMed
description Virus isolates are not only useful for diagnosing infections, e.g., respiratory syncytial virus (RSV), but can also facilitate many aspects of practical viral studies such as analyses of antigenicity and the action mechanisms of antivirals, among others. We have been isolating RSV from clinical specimens from patients with respiratory symptoms every year since our first isolation of RSV in 1964, and isolation rates have varied considerably over the years. As collected clinical specimens are conventionally stored in a refrigerator from collection to inoculation into cells, we hypothesized that certain storage conditions or associated factors might account for these differences. Hence, we evaluated the thermal stability of a total of 64 viruses isolated from 1998 to 2018 upon storage at 4 °C and 20 °C for a defined duration. Interestingly, and contrary to our current understanding, 22 strains (34%) showed a greater loss of viability upon short-term storage at 4 °C than at 20 °C. Thirty-seven strains (57%) showed an almost equal loss, and only five strains (8%) were more stable at 4 °C than at 20 °C. This finding warrants reconsideration of the temperature for the temporary storage of clinical samples for RSV isolation.
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spelling pubmed-90294762022-04-23 Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions Kitai, Yuki Sato, Ko Shirato, Kazuya Ohmiya, Suguru Watanabe, Oshi Kisu, Tomoko Ota, Reiko Takeda, Makoto Kawakami, Kazuyoshi Nishimura, Hidekazu Viruses Communication Virus isolates are not only useful for diagnosing infections, e.g., respiratory syncytial virus (RSV), but can also facilitate many aspects of practical viral studies such as analyses of antigenicity and the action mechanisms of antivirals, among others. We have been isolating RSV from clinical specimens from patients with respiratory symptoms every year since our first isolation of RSV in 1964, and isolation rates have varied considerably over the years. As collected clinical specimens are conventionally stored in a refrigerator from collection to inoculation into cells, we hypothesized that certain storage conditions or associated factors might account for these differences. Hence, we evaluated the thermal stability of a total of 64 viruses isolated from 1998 to 2018 upon storage at 4 °C and 20 °C for a defined duration. Interestingly, and contrary to our current understanding, 22 strains (34%) showed a greater loss of viability upon short-term storage at 4 °C than at 20 °C. Thirty-seven strains (57%) showed an almost equal loss, and only five strains (8%) were more stable at 4 °C than at 20 °C. This finding warrants reconsideration of the temperature for the temporary storage of clinical samples for RSV isolation. MDPI 2022-03-25 /pmc/articles/PMC9029476/ /pubmed/35458409 http://dx.doi.org/10.3390/v14040679 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kitai, Yuki
Sato, Ko
Shirato, Kazuya
Ohmiya, Suguru
Watanabe, Oshi
Kisu, Tomoko
Ota, Reiko
Takeda, Makoto
Kawakami, Kazuyoshi
Nishimura, Hidekazu
Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions
title Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions
title_full Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions
title_fullStr Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions
title_full_unstemmed Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions
title_short Variation in Thermal Stability among Respiratory Syncytial Virus Clinical Isolates under Non-Freezing Conditions
title_sort variation in thermal stability among respiratory syncytial virus clinical isolates under non-freezing conditions
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029476/
https://www.ncbi.nlm.nih.gov/pubmed/35458409
http://dx.doi.org/10.3390/v14040679
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