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Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina

Human papillomavirus (HPV) testing using self-collected vaginal specimens is the preferred choice to increase screening uptake. Although the HPV testing results of these samples depend on the cells that naturally exfoliate from the cervical lesion and drift into the vagina, the mechanism of when and...

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Autores principales: Okodo, Mitsuaki, Okayama, Kaori, Teruya, Koji, Tanabe, Kazumasa, Ito, Chieko, Ishii, Yasuyoshi, Fujii, Masahiko, Kimura, Hirokazu, Oda, Mizue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029653/
https://www.ncbi.nlm.nih.gov/pubmed/35456745
http://dx.doi.org/10.3390/microorganisms10040693
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author Okodo, Mitsuaki
Okayama, Kaori
Teruya, Koji
Tanabe, Kazumasa
Ito, Chieko
Ishii, Yasuyoshi
Fujii, Masahiko
Kimura, Hirokazu
Oda, Mizue
author_facet Okodo, Mitsuaki
Okayama, Kaori
Teruya, Koji
Tanabe, Kazumasa
Ito, Chieko
Ishii, Yasuyoshi
Fujii, Masahiko
Kimura, Hirokazu
Oda, Mizue
author_sort Okodo, Mitsuaki
collection PubMed
description Human papillomavirus (HPV) testing using self-collected vaginal specimens is the preferred choice to increase screening uptake. Although the HPV testing results of these samples depend on the cells that naturally exfoliate from the cervical lesion and drift into the vagina, the mechanism of when and how these exfoliated cells mix with the self-collected sample remains unclear. Hence, the study aimed to clarify the relationship between the vaginal drift of HPV-infected cells exfoliated from the cervix, and the menstrual cycle. A total of 180 scraped samples of the cervix and vagina were examined. The exfoliated cells were classified into two categories according to the HPV genotyping results of each sample: sufficient accumulation (same HPV types in cervical and vaginal samples) and insufficient accumulation (fewer HPV types in vaginal samples than in cervical samples, or HPV positivity in cervical samples and HPV negativity in vaginal samples). A moderately strong statistically significant association was observed between exfoliated cell accumulation and the menstrual cycle, and insufficient accumulation was statistically significantly increased at the early proliferative phases. Self-collection of vaginal samples at the early proliferation phase indicates insufficient sample quantities or lower viral load, thereby affecting HPV genotyping.
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spelling pubmed-90296532022-04-23 Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina Okodo, Mitsuaki Okayama, Kaori Teruya, Koji Tanabe, Kazumasa Ito, Chieko Ishii, Yasuyoshi Fujii, Masahiko Kimura, Hirokazu Oda, Mizue Microorganisms Article Human papillomavirus (HPV) testing using self-collected vaginal specimens is the preferred choice to increase screening uptake. Although the HPV testing results of these samples depend on the cells that naturally exfoliate from the cervical lesion and drift into the vagina, the mechanism of when and how these exfoliated cells mix with the self-collected sample remains unclear. Hence, the study aimed to clarify the relationship between the vaginal drift of HPV-infected cells exfoliated from the cervix, and the menstrual cycle. A total of 180 scraped samples of the cervix and vagina were examined. The exfoliated cells were classified into two categories according to the HPV genotyping results of each sample: sufficient accumulation (same HPV types in cervical and vaginal samples) and insufficient accumulation (fewer HPV types in vaginal samples than in cervical samples, or HPV positivity in cervical samples and HPV negativity in vaginal samples). A moderately strong statistically significant association was observed between exfoliated cell accumulation and the menstrual cycle, and insufficient accumulation was statistically significantly increased at the early proliferative phases. Self-collection of vaginal samples at the early proliferation phase indicates insufficient sample quantities or lower viral load, thereby affecting HPV genotyping. MDPI 2022-03-23 /pmc/articles/PMC9029653/ /pubmed/35456745 http://dx.doi.org/10.3390/microorganisms10040693 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Okodo, Mitsuaki
Okayama, Kaori
Teruya, Koji
Tanabe, Kazumasa
Ito, Chieko
Ishii, Yasuyoshi
Fujii, Masahiko
Kimura, Hirokazu
Oda, Mizue
Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina
title Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina
title_full Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina
title_fullStr Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina
title_full_unstemmed Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina
title_short Effects of Menstrual Cycle on the Accumulation of Human Papillomavirus-Infected Cells Exfoliated from the Cervix That Drift into the Vagina
title_sort effects of menstrual cycle on the accumulation of human papillomavirus-infected cells exfoliated from the cervix that drift into the vagina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029653/
https://www.ncbi.nlm.nih.gov/pubmed/35456745
http://dx.doi.org/10.3390/microorganisms10040693
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