Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor

In only two years, the coronavirus disease 2019 (COVID-19) pandemic has had a devastating effect on public health all over the world and caused irreparable economic damage across all countries. Due to the limited therapeutic management of COVID-19 and the lack of tailor-made antiviral agents, findin...

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Autores principales: Nemethova, Veronika, Mazancova, Petra, Selc, Michal, Jakic, Kristina, Uhelska, Lucia, Nemethova, Boglarka, Poturnayova, Alexandra, Drgona, Lubos, Babelova, Andrea, Razga, Filip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029688/
https://www.ncbi.nlm.nih.gov/pubmed/35458415
http://dx.doi.org/10.3390/v14040685
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author Nemethova, Veronika
Mazancova, Petra
Selc, Michal
Jakic, Kristina
Uhelska, Lucia
Nemethova, Boglarka
Poturnayova, Alexandra
Drgona, Lubos
Babelova, Andrea
Razga, Filip
author_facet Nemethova, Veronika
Mazancova, Petra
Selc, Michal
Jakic, Kristina
Uhelska, Lucia
Nemethova, Boglarka
Poturnayova, Alexandra
Drgona, Lubos
Babelova, Andrea
Razga, Filip
author_sort Nemethova, Veronika
collection PubMed
description In only two years, the coronavirus disease 2019 (COVID-19) pandemic has had a devastating effect on public health all over the world and caused irreparable economic damage across all countries. Due to the limited therapeutic management of COVID-19 and the lack of tailor-made antiviral agents, finding new methods to combat this viral illness is now a priority. Herein, we report on a specific oligonucleotide-based RNA inhibitor targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It displayed remarkable spontaneous cellular uptake, >94% efficiency in reducing RNA-dependent RNA polymerase (RdRp) RNA levels in transfected lung cell lines, and >98% efficiency in reducing SARS-CoV-2 RNA levels in samples from patients hospitalized with COVID-19 following a single application.
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spelling pubmed-90296882022-04-23 Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor Nemethova, Veronika Mazancova, Petra Selc, Michal Jakic, Kristina Uhelska, Lucia Nemethova, Boglarka Poturnayova, Alexandra Drgona, Lubos Babelova, Andrea Razga, Filip Viruses Communication In only two years, the coronavirus disease 2019 (COVID-19) pandemic has had a devastating effect on public health all over the world and caused irreparable economic damage across all countries. Due to the limited therapeutic management of COVID-19 and the lack of tailor-made antiviral agents, finding new methods to combat this viral illness is now a priority. Herein, we report on a specific oligonucleotide-based RNA inhibitor targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It displayed remarkable spontaneous cellular uptake, >94% efficiency in reducing RNA-dependent RNA polymerase (RdRp) RNA levels in transfected lung cell lines, and >98% efficiency in reducing SARS-CoV-2 RNA levels in samples from patients hospitalized with COVID-19 following a single application. MDPI 2022-03-25 /pmc/articles/PMC9029688/ /pubmed/35458415 http://dx.doi.org/10.3390/v14040685 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nemethova, Veronika
Mazancova, Petra
Selc, Michal
Jakic, Kristina
Uhelska, Lucia
Nemethova, Boglarka
Poturnayova, Alexandra
Drgona, Lubos
Babelova, Andrea
Razga, Filip
Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor
title Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor
title_full Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor
title_fullStr Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor
title_full_unstemmed Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor
title_short Effective Reduction of SARS-CoV-2 RNA Levels Using a Tailor-Made Oligonucleotide-Based RNA Inhibitor
title_sort effective reduction of sars-cov-2 rna levels using a tailor-made oligonucleotide-based rna inhibitor
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029688/
https://www.ncbi.nlm.nih.gov/pubmed/35458415
http://dx.doi.org/10.3390/v14040685
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