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Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A

Keratin liposomes have emerged as a useful topical drug delivery system given theirenhanced ability to penetrate the skin, making them ideal as topical drug vehicles. However, the mechanisms of the drug penetration enhancement of keratin liposomes have not been clearly elucidated. Therefore, licocha...

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Autores principales: Wu, Wenfeng, Wang, Zhuxian, Wu, Yufan, Wu, Huiyi, Chen, Tingting, Xue, Yaqi, Wang, Yuan, Jiang, Cuiping, Shen, Chunyan, Liu, Li, Zhu, Hongxia, Liu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029797/
https://www.ncbi.nlm.nih.gov/pubmed/35458701
http://dx.doi.org/10.3390/molecules27082504
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author Wu, Wenfeng
Wang, Zhuxian
Wu, Yufan
Wu, Huiyi
Chen, Tingting
Xue, Yaqi
Wang, Yuan
Jiang, Cuiping
Shen, Chunyan
Liu, Li
Zhu, Hongxia
Liu, Qiang
author_facet Wu, Wenfeng
Wang, Zhuxian
Wu, Yufan
Wu, Huiyi
Chen, Tingting
Xue, Yaqi
Wang, Yuan
Jiang, Cuiping
Shen, Chunyan
Liu, Li
Zhu, Hongxia
Liu, Qiang
author_sort Wu, Wenfeng
collection PubMed
description Keratin liposomes have emerged as a useful topical drug delivery system given theirenhanced ability to penetrate the skin, making them ideal as topical drug vehicles. However, the mechanisms of the drug penetration enhancement of keratin liposomes have not been clearly elucidated. Therefore, licochalcone A(LA)-loaded skin keratin liposomes (LALs) were prepared to investigate their mechanisms of penetration enhancement on the skin and inB16F10 cells. Skin deposition studies, differential scanning calorimetry (DSC), attenuated total reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR), and skin distribution and intracellular distribution studies were carried out to demonstrate the drug enhancement mechanisms of LALs. We found that the optimal application of LALs enhanced drug permeation via alterations in the components, structure, and thermodynamic properties of the stratum corneum (SC), that is, by enhancing the lipid fluidization, altering the skin keratin, and changing the thermodynamic properties of the SC. Moreover, hair follicles were the main penetration pathways for the LA delivery, which occurred in a time-dependent manner. In the B16F10 cells, the skin keratin liposomes effectively delivered LA into the cytoplasm without cytotoxicity. Thus, LAL nanoparticles are promising topical drug delivery systems for pharmaceutical and cosmetic applications.
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spelling pubmed-90297972022-04-23 Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A Wu, Wenfeng Wang, Zhuxian Wu, Yufan Wu, Huiyi Chen, Tingting Xue, Yaqi Wang, Yuan Jiang, Cuiping Shen, Chunyan Liu, Li Zhu, Hongxia Liu, Qiang Molecules Article Keratin liposomes have emerged as a useful topical drug delivery system given theirenhanced ability to penetrate the skin, making them ideal as topical drug vehicles. However, the mechanisms of the drug penetration enhancement of keratin liposomes have not been clearly elucidated. Therefore, licochalcone A(LA)-loaded skin keratin liposomes (LALs) were prepared to investigate their mechanisms of penetration enhancement on the skin and inB16F10 cells. Skin deposition studies, differential scanning calorimetry (DSC), attenuated total reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR), and skin distribution and intracellular distribution studies were carried out to demonstrate the drug enhancement mechanisms of LALs. We found that the optimal application of LALs enhanced drug permeation via alterations in the components, structure, and thermodynamic properties of the stratum corneum (SC), that is, by enhancing the lipid fluidization, altering the skin keratin, and changing the thermodynamic properties of the SC. Moreover, hair follicles were the main penetration pathways for the LA delivery, which occurred in a time-dependent manner. In the B16F10 cells, the skin keratin liposomes effectively delivered LA into the cytoplasm without cytotoxicity. Thus, LAL nanoparticles are promising topical drug delivery systems for pharmaceutical and cosmetic applications. MDPI 2022-04-13 /pmc/articles/PMC9029797/ /pubmed/35458701 http://dx.doi.org/10.3390/molecules27082504 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Wenfeng
Wang, Zhuxian
Wu, Yufan
Wu, Huiyi
Chen, Tingting
Xue, Yaqi
Wang, Yuan
Jiang, Cuiping
Shen, Chunyan
Liu, Li
Zhu, Hongxia
Liu, Qiang
Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A
title Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A
title_full Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A
title_fullStr Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A
title_full_unstemmed Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A
title_short Mechanisms of Penetration Enhancement and Transport Utilizing Skin Keratine Liposomes for the Topical Delivery of Licochalcone A
title_sort mechanisms of penetration enhancement and transport utilizing skin keratine liposomes for the topical delivery of licochalcone a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029797/
https://www.ncbi.nlm.nih.gov/pubmed/35458701
http://dx.doi.org/10.3390/molecules27082504
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