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Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence

We developed an artificial intelligence (AI) model that evaluates the feasibility of AI-assisted multiparameter flow cytometry (MFC) diagnosis of acute leukemia. Two hundred acute leukemia patients and 94 patients with cytopenia(s) or hematocytosis were selected to study the AI application in MFC di...

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Autores principales: Zhong, Pengqiang, Hong, Mengzhi, He, Huanyu, Zhang, Jiang, Chen, Yaoming, Wang, Zhigang, Chen, Peisong, Ouyang, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029950/
https://www.ncbi.nlm.nih.gov/pubmed/35453875
http://dx.doi.org/10.3390/diagnostics12040827
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author Zhong, Pengqiang
Hong, Mengzhi
He, Huanyu
Zhang, Jiang
Chen, Yaoming
Wang, Zhigang
Chen, Peisong
Ouyang, Juan
author_facet Zhong, Pengqiang
Hong, Mengzhi
He, Huanyu
Zhang, Jiang
Chen, Yaoming
Wang, Zhigang
Chen, Peisong
Ouyang, Juan
author_sort Zhong, Pengqiang
collection PubMed
description We developed an artificial intelligence (AI) model that evaluates the feasibility of AI-assisted multiparameter flow cytometry (MFC) diagnosis of acute leukemia. Two hundred acute leukemia patients and 94 patients with cytopenia(s) or hematocytosis were selected to study the AI application in MFC diagnosis of acute leukemia. The kappa test analyzed the consistency of the diagnostic results and the immunophenotype of acute leukemia. Bland–Altman and Pearson analyses evaluated the consistency and correlation of the abnormal cell proportion between the AI and manual methods. The AI analysis time for each case (83.72 ± 23.90 s, mean ± SD) was significantly shorter than the average time for manual analysis (15.64 ± 7.16 min, mean ± SD). The total consistency of diagnostic results was 0.976 (kappa (κ) = 0.963). The Bland–Altman evaluation of the abnormal cell proportion between the AI analysis and manual analysis showed that the bias ± SD was 0.752 ± 6.646, and the 95% limit of agreement was from −12.775 to 13.779 (p = 0.1225). The total consistency of the AI immunophenotypic diagnosis and the manual results was 0.889 (kappa, 0.775). The consistency and speedup of the AI-assisted workflow indicate its promising clinical application.
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spelling pubmed-90299502022-04-23 Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence Zhong, Pengqiang Hong, Mengzhi He, Huanyu Zhang, Jiang Chen, Yaoming Wang, Zhigang Chen, Peisong Ouyang, Juan Diagnostics (Basel) Article We developed an artificial intelligence (AI) model that evaluates the feasibility of AI-assisted multiparameter flow cytometry (MFC) diagnosis of acute leukemia. Two hundred acute leukemia patients and 94 patients with cytopenia(s) or hematocytosis were selected to study the AI application in MFC diagnosis of acute leukemia. The kappa test analyzed the consistency of the diagnostic results and the immunophenotype of acute leukemia. Bland–Altman and Pearson analyses evaluated the consistency and correlation of the abnormal cell proportion between the AI and manual methods. The AI analysis time for each case (83.72 ± 23.90 s, mean ± SD) was significantly shorter than the average time for manual analysis (15.64 ± 7.16 min, mean ± SD). The total consistency of diagnostic results was 0.976 (kappa (κ) = 0.963). The Bland–Altman evaluation of the abnormal cell proportion between the AI analysis and manual analysis showed that the bias ± SD was 0.752 ± 6.646, and the 95% limit of agreement was from −12.775 to 13.779 (p = 0.1225). The total consistency of the AI immunophenotypic diagnosis and the manual results was 0.889 (kappa, 0.775). The consistency and speedup of the AI-assisted workflow indicate its promising clinical application. MDPI 2022-03-28 /pmc/articles/PMC9029950/ /pubmed/35453875 http://dx.doi.org/10.3390/diagnostics12040827 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhong, Pengqiang
Hong, Mengzhi
He, Huanyu
Zhang, Jiang
Chen, Yaoming
Wang, Zhigang
Chen, Peisong
Ouyang, Juan
Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence
title Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence
title_full Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence
title_fullStr Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence
title_full_unstemmed Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence
title_short Diagnosis of Acute Leukemia by Multiparameter Flow Cytometry with the Assistance of Artificial Intelligence
title_sort diagnosis of acute leukemia by multiparameter flow cytometry with the assistance of artificial intelligence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029950/
https://www.ncbi.nlm.nih.gov/pubmed/35453875
http://dx.doi.org/10.3390/diagnostics12040827
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