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Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication

A comparative analysis of Gaultheria fragrantissima (Ericaceae) essential oils based on geographical location, distillation time, and varying distillation conditions was carried out, and their compositions were evaluated by gas chromatography–mass spectrometry (GC–MS), chiral GC–MS, and gas chromato...

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Autores principales: Ojha, Pawan Kumar, Poudel, Darbin Kumar, Dangol, Sabita, Rokaya, Anil, Timsina, Sujan, Satyal, Prabodh, Setzer, William N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030118/
https://www.ncbi.nlm.nih.gov/pubmed/35448818
http://dx.doi.org/10.3390/plants11081090
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author Ojha, Pawan Kumar
Poudel, Darbin Kumar
Dangol, Sabita
Rokaya, Anil
Timsina, Sujan
Satyal, Prabodh
Setzer, William N.
author_facet Ojha, Pawan Kumar
Poudel, Darbin Kumar
Dangol, Sabita
Rokaya, Anil
Timsina, Sujan
Satyal, Prabodh
Setzer, William N.
author_sort Ojha, Pawan Kumar
collection PubMed
description A comparative analysis of Gaultheria fragrantissima (Ericaceae) essential oils based on geographical location, distillation time, and varying distillation conditions was carried out, and their compositions were evaluated by gas chromatography–mass spectrometry (GC–MS), chiral GC–MS, and gas chromatography–flame ionization detection (GC–FID). In addition, each of seven commercial wintergreen essential oil samples from Nepal and China were analyzed. The highest extraction yield was 1.48% and the maximum number of compounds identified in natural wintergreen oil was twenty-two. Based on distillation time, the maximum numbers of identified compounds are present in 120 min. Linalool, phenol, vetispirane, and ethyl salicylate were present in commercial wintergreen oils both from Nepal and China. The presence of compounds such as elsholtzia ketone and β-dehydroelsholtzia ketone in the China samples represented a significant difference in wintergreen oil between the two geographical sources. Dimethyl 2-hydroxyterephthalate is a well-known synthetic marker for wintergreen oil when synthesis is carried out using salicylic acid, but the synthetic marker was absent while using acetylsalicylic acid as a precursor during synthesis. Adulteration analysis of wintergreen oil showed an increase in the concentration of dimethyl 2-hydroxyterephthalate, whereas the concentrations of minor components decreased and methyl salicylate remained unchanged. To the best of our knowledge, this is the first report of the enantioselective analysis of wintergreen essential oil. Furthermore, three samples showed notable antibacterial activity against Staphylococcus epidermidis, with an MIC value of 156.3 μg/mL. Similarly, one sample showed effectiveness against Aspergillus niger (MIC = 78.1 μg/mL).
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spelling pubmed-90301182022-04-23 Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication Ojha, Pawan Kumar Poudel, Darbin Kumar Dangol, Sabita Rokaya, Anil Timsina, Sujan Satyal, Prabodh Setzer, William N. Plants (Basel) Article A comparative analysis of Gaultheria fragrantissima (Ericaceae) essential oils based on geographical location, distillation time, and varying distillation conditions was carried out, and their compositions were evaluated by gas chromatography–mass spectrometry (GC–MS), chiral GC–MS, and gas chromatography–flame ionization detection (GC–FID). In addition, each of seven commercial wintergreen essential oil samples from Nepal and China were analyzed. The highest extraction yield was 1.48% and the maximum number of compounds identified in natural wintergreen oil was twenty-two. Based on distillation time, the maximum numbers of identified compounds are present in 120 min. Linalool, phenol, vetispirane, and ethyl salicylate were present in commercial wintergreen oils both from Nepal and China. The presence of compounds such as elsholtzia ketone and β-dehydroelsholtzia ketone in the China samples represented a significant difference in wintergreen oil between the two geographical sources. Dimethyl 2-hydroxyterephthalate is a well-known synthetic marker for wintergreen oil when synthesis is carried out using salicylic acid, but the synthetic marker was absent while using acetylsalicylic acid as a precursor during synthesis. Adulteration analysis of wintergreen oil showed an increase in the concentration of dimethyl 2-hydroxyterephthalate, whereas the concentrations of minor components decreased and methyl salicylate remained unchanged. To the best of our knowledge, this is the first report of the enantioselective analysis of wintergreen essential oil. Furthermore, three samples showed notable antibacterial activity against Staphylococcus epidermidis, with an MIC value of 156.3 μg/mL. Similarly, one sample showed effectiveness against Aspergillus niger (MIC = 78.1 μg/mL). MDPI 2022-04-17 /pmc/articles/PMC9030118/ /pubmed/35448818 http://dx.doi.org/10.3390/plants11081090 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ojha, Pawan Kumar
Poudel, Darbin Kumar
Dangol, Sabita
Rokaya, Anil
Timsina, Sujan
Satyal, Prabodh
Setzer, William N.
Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication
title Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication
title_full Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication
title_fullStr Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication
title_full_unstemmed Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication
title_short Volatile Constituent Analysis of Wintergreen Essential Oil and Comparison with Synthetic Methyl Salicylate for Authentication
title_sort volatile constituent analysis of wintergreen essential oil and comparison with synthetic methyl salicylate for authentication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030118/
https://www.ncbi.nlm.nih.gov/pubmed/35448818
http://dx.doi.org/10.3390/plants11081090
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