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Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner
The low efficacy and side effects associated with antifungal agents have highlighted the importance of developing immunotherapeutic approaches to treat Cryptococcus gattii infection. We developed an immunization strategy that uses selective Dectin-1 agonist as an adjuvant. BALB/c or C57BL/6 mice rec...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030172/ https://www.ncbi.nlm.nih.gov/pubmed/35455369 http://dx.doi.org/10.3390/vaccines10040620 |
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author | Oliveira-Brito, Patrícia Kellen Martins de Campos, Gabriela Yamazaki Guimarães, Júlia Garcia Serafim da Costa, Letícia Silva de Moura, Edanielle Lazo-Chica, Javier Emílio Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido |
author_facet | Oliveira-Brito, Patrícia Kellen Martins de Campos, Gabriela Yamazaki Guimarães, Júlia Garcia Serafim da Costa, Letícia Silva de Moura, Edanielle Lazo-Chica, Javier Emílio Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido |
author_sort | Oliveira-Brito, Patrícia Kellen Martins |
collection | PubMed |
description | The low efficacy and side effects associated with antifungal agents have highlighted the importance of developing immunotherapeutic approaches to treat Cryptococcus gattii infection. We developed an immunization strategy that uses selective Dectin-1 agonist as an adjuvant. BALB/c or C57BL/6 mice received curdlan or β-glucan peptide (BGP) before immunization with heat-killed C. gattii, and the mice were infected with viable C. gattii on day 14 post immunization and euthanized 14 days after infection. Adjuvant curdlan restored pulmonary tumor necrosis factor- α (TNF-α) levels, as induced by immunization with heat-killed C. gattii. The average area and relative frequency of C. gattii titan cells in the lungs of curdlan-treated BALB/c mice were reduced. However, this did not reduce the pulmonary fungal burden or decrease the i0,nflammatory infiltrate in the pulmonary parenchyma of BALB/c mice. Conversely, adjuvant curdlan induced high levels of interferon-γ (IFN-γ) and interleukin (IL)-10 and decreased the C. gattii burden in the lungs of C57BL/6 mice, which was not replicated in β-glucan peptide-treated mice. The adjuvant curdlan favors the control of C. gattii infection depending on the immune response profile of the mouse strain. This study will have implications for developing new immunotherapeutic approaches to treat C. gattii infection. |
format | Online Article Text |
id | pubmed-9030172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90301722022-04-23 Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner Oliveira-Brito, Patrícia Kellen Martins de Campos, Gabriela Yamazaki Guimarães, Júlia Garcia Serafim da Costa, Letícia Silva de Moura, Edanielle Lazo-Chica, Javier Emílio Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido Vaccines (Basel) Article The low efficacy and side effects associated with antifungal agents have highlighted the importance of developing immunotherapeutic approaches to treat Cryptococcus gattii infection. We developed an immunization strategy that uses selective Dectin-1 agonist as an adjuvant. BALB/c or C57BL/6 mice received curdlan or β-glucan peptide (BGP) before immunization with heat-killed C. gattii, and the mice were infected with viable C. gattii on day 14 post immunization and euthanized 14 days after infection. Adjuvant curdlan restored pulmonary tumor necrosis factor- α (TNF-α) levels, as induced by immunization with heat-killed C. gattii. The average area and relative frequency of C. gattii titan cells in the lungs of curdlan-treated BALB/c mice were reduced. However, this did not reduce the pulmonary fungal burden or decrease the i0,nflammatory infiltrate in the pulmonary parenchyma of BALB/c mice. Conversely, adjuvant curdlan induced high levels of interferon-γ (IFN-γ) and interleukin (IL)-10 and decreased the C. gattii burden in the lungs of C57BL/6 mice, which was not replicated in β-glucan peptide-treated mice. The adjuvant curdlan favors the control of C. gattii infection depending on the immune response profile of the mouse strain. This study will have implications for developing new immunotherapeutic approaches to treat C. gattii infection. MDPI 2022-04-15 /pmc/articles/PMC9030172/ /pubmed/35455369 http://dx.doi.org/10.3390/vaccines10040620 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Oliveira-Brito, Patrícia Kellen Martins de Campos, Gabriela Yamazaki Guimarães, Júlia Garcia Serafim da Costa, Letícia Silva de Moura, Edanielle Lazo-Chica, Javier Emílio Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner |
title | Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner |
title_full | Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner |
title_fullStr | Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner |
title_full_unstemmed | Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner |
title_short | Adjuvant Curdlan Contributes to Immunization against Cryptococcus gattii Infection in a Mouse Strain-Specific Manner |
title_sort | adjuvant curdlan contributes to immunization against cryptococcus gattii infection in a mouse strain-specific manner |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030172/ https://www.ncbi.nlm.nih.gov/pubmed/35455369 http://dx.doi.org/10.3390/vaccines10040620 |
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