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Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols

Mechanisms of exercise-induced muscle injury with etiopathogenesis and its consequences have been described; however, the impact of different intensities of exercise on the mechanisms of muscular injury development is not well understood. The aim of this study was to exploit the relationship between...

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Autores principales: D’Amico, Alessandra, Cavarretta, Elena, Fossati, Chiara, Borrione, Paolo, Pigozzi, Fabio, Frati, Giacomo, Sciarretta, Sebastiano, Costa, Vincenzo, De Grandis, Fabrizio, Nigro, Antonia, Peruzzi, Mariangela, Miraldi, Fabio, Saade, Wael, Calogero, Antonella, Rosa, Paolo, Galardo, Gioacchino, Loffredo, Lorenzo, Pignatelli, Pasquale, Nocella, Cristina, Carnevale, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030438/
https://www.ncbi.nlm.nih.gov/pubmed/35458119
http://dx.doi.org/10.3390/nu14081558
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author D’Amico, Alessandra
Cavarretta, Elena
Fossati, Chiara
Borrione, Paolo
Pigozzi, Fabio
Frati, Giacomo
Sciarretta, Sebastiano
Costa, Vincenzo
De Grandis, Fabrizio
Nigro, Antonia
Peruzzi, Mariangela
Miraldi, Fabio
Saade, Wael
Calogero, Antonella
Rosa, Paolo
Galardo, Gioacchino
Loffredo, Lorenzo
Pignatelli, Pasquale
Nocella, Cristina
Carnevale, Roberto
author_facet D’Amico, Alessandra
Cavarretta, Elena
Fossati, Chiara
Borrione, Paolo
Pigozzi, Fabio
Frati, Giacomo
Sciarretta, Sebastiano
Costa, Vincenzo
De Grandis, Fabrizio
Nigro, Antonia
Peruzzi, Mariangela
Miraldi, Fabio
Saade, Wael
Calogero, Antonella
Rosa, Paolo
Galardo, Gioacchino
Loffredo, Lorenzo
Pignatelli, Pasquale
Nocella, Cristina
Carnevale, Roberto
author_sort D’Amico, Alessandra
collection PubMed
description Mechanisms of exercise-induced muscle injury with etiopathogenesis and its consequences have been described; however, the impact of different intensities of exercise on the mechanisms of muscular injury development is not well understood. The aim of this study was to exploit the relationship between platelet activation, oxidative stress and muscular injuries induced by physical exercise in elite football players compared to amateur athletes. Oxidant/antioxidant status, platelet activation and markers of muscle damage were evaluated in 23 elite football players and 23 amateur athletes. Compared to amateurs, elite football players showed lower antioxidant capacity and higher oxidative stress paralleled by increased platelet activation and muscle damage markers. Simple linear regression analysis showed that sNOX2-dp and H(2)O(2), sCD40L and PDGF-bb were associated with a significant increase in muscle damage biomarkers. In vitro studies also showed that plasma obtained from elite athletes increased oxidative stress and muscle damage in human skeletal muscle myoblasts cell line compared to amateurs’ plasma, an effect blunted by the NOX2 inhibitor or by the cell treatment with cocoa-derived polyphenols. These results indicate that platelet activation increased muscular injuries induced by oxidative stress. Moreover, NOX2 inhibition and polyphenol extracts treatment positively modulates redox status and reduce exercise-induced muscular injury.
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spelling pubmed-90304382022-04-23 Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols D’Amico, Alessandra Cavarretta, Elena Fossati, Chiara Borrione, Paolo Pigozzi, Fabio Frati, Giacomo Sciarretta, Sebastiano Costa, Vincenzo De Grandis, Fabrizio Nigro, Antonia Peruzzi, Mariangela Miraldi, Fabio Saade, Wael Calogero, Antonella Rosa, Paolo Galardo, Gioacchino Loffredo, Lorenzo Pignatelli, Pasquale Nocella, Cristina Carnevale, Roberto Nutrients Article Mechanisms of exercise-induced muscle injury with etiopathogenesis and its consequences have been described; however, the impact of different intensities of exercise on the mechanisms of muscular injury development is not well understood. The aim of this study was to exploit the relationship between platelet activation, oxidative stress and muscular injuries induced by physical exercise in elite football players compared to amateur athletes. Oxidant/antioxidant status, platelet activation and markers of muscle damage were evaluated in 23 elite football players and 23 amateur athletes. Compared to amateurs, elite football players showed lower antioxidant capacity and higher oxidative stress paralleled by increased platelet activation and muscle damage markers. Simple linear regression analysis showed that sNOX2-dp and H(2)O(2), sCD40L and PDGF-bb were associated with a significant increase in muscle damage biomarkers. In vitro studies also showed that plasma obtained from elite athletes increased oxidative stress and muscle damage in human skeletal muscle myoblasts cell line compared to amateurs’ plasma, an effect blunted by the NOX2 inhibitor or by the cell treatment with cocoa-derived polyphenols. These results indicate that platelet activation increased muscular injuries induced by oxidative stress. Moreover, NOX2 inhibition and polyphenol extracts treatment positively modulates redox status and reduce exercise-induced muscular injury. MDPI 2022-04-08 /pmc/articles/PMC9030438/ /pubmed/35458119 http://dx.doi.org/10.3390/nu14081558 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Amico, Alessandra
Cavarretta, Elena
Fossati, Chiara
Borrione, Paolo
Pigozzi, Fabio
Frati, Giacomo
Sciarretta, Sebastiano
Costa, Vincenzo
De Grandis, Fabrizio
Nigro, Antonia
Peruzzi, Mariangela
Miraldi, Fabio
Saade, Wael
Calogero, Antonella
Rosa, Paolo
Galardo, Gioacchino
Loffredo, Lorenzo
Pignatelli, Pasquale
Nocella, Cristina
Carnevale, Roberto
Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
title Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
title_full Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
title_fullStr Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
title_full_unstemmed Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
title_short Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
title_sort platelet activation favours nox2-mediated muscle damage in elite athletes: the role of cocoa-derived polyphenols
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030438/
https://www.ncbi.nlm.nih.gov/pubmed/35458119
http://dx.doi.org/10.3390/nu14081558
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