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FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster

SIMPLE SUMMARY: Two endocrine hormones, ecdysone and juvenile hormone (JH), control insect development and reproduction. Some studies in the literature have suggested that FKBP39 functions as a transcriptional factor and regulates the JH pathway in Drosophila. However, the physiological roles of FKB...

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Autores principales: Wang, Xinyu, Zhou, Ying, Guan, Jianwen, Cheng, Yang, Lu, Yingying, Wei, Youheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030728/
https://www.ncbi.nlm.nih.gov/pubmed/35447772
http://dx.doi.org/10.3390/insects13040330
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author Wang, Xinyu
Zhou, Ying
Guan, Jianwen
Cheng, Yang
Lu, Yingying
Wei, Youheng
author_facet Wang, Xinyu
Zhou, Ying
Guan, Jianwen
Cheng, Yang
Lu, Yingying
Wei, Youheng
author_sort Wang, Xinyu
collection PubMed
description SIMPLE SUMMARY: Two endocrine hormones, ecdysone and juvenile hormone (JH), control insect development and reproduction. Some studies in the literature have suggested that FKBP39 functions as a transcriptional factor and regulates the JH pathway in Drosophila. However, the physiological roles of FKBP39 are still elusive. To determine the FKBP39 roles in vivo, we first developed an antibody to check the FKBP39 expression pattern and then detected JH activity-related phenotypes in fkbp39 mutants, such as pupariation, reproduction, and Kr-h1 expression. We found that FKBP39 expresses at a high level and controls JH activity at the larval stage. Moreover, we found that rp49, the most widely used reference gene for Real-time quantitative PCR (qRT-PCR), significantly decreased in the fkbp39 mutant. This work will provide valuable information for studies on JH activity and insect development. ABSTRACT: FK506-binding protein 39kD (FKBP39) localizes in the nucleus and contains multiple functional domains. Structural analysis suggests that FKBP39 might function as a transcriptional factor and control juvenile hormone (JH) activity. Here, we show that FKBP39 expresses at a high level and localizes in the nucleolus of fat body cells during the first two larval stages and early third larval stage. The fkbp39 mutant displays delayed larval-pupal transition and an increased expression of Kr-h1, the main mediator of the JH pathway, at the early third larval stage. Moreover, the fkbp39 mutant has a fertility defect that is independent of JH activity. Interestingly, the expression of rp49, the most widely used reference gene for qRT-PCR in Drosophila, significantly decreased in the fkbp39 mutant, suggesting that FKBP39 might regulate ribosome assembly. Taken together, our data demonstrate the expression pattern and physiological roles of FKBP39 in Drosophila.
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spelling pubmed-90307282022-04-23 FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster Wang, Xinyu Zhou, Ying Guan, Jianwen Cheng, Yang Lu, Yingying Wei, Youheng Insects Article SIMPLE SUMMARY: Two endocrine hormones, ecdysone and juvenile hormone (JH), control insect development and reproduction. Some studies in the literature have suggested that FKBP39 functions as a transcriptional factor and regulates the JH pathway in Drosophila. However, the physiological roles of FKBP39 are still elusive. To determine the FKBP39 roles in vivo, we first developed an antibody to check the FKBP39 expression pattern and then detected JH activity-related phenotypes in fkbp39 mutants, such as pupariation, reproduction, and Kr-h1 expression. We found that FKBP39 expresses at a high level and controls JH activity at the larval stage. Moreover, we found that rp49, the most widely used reference gene for Real-time quantitative PCR (qRT-PCR), significantly decreased in the fkbp39 mutant. This work will provide valuable information for studies on JH activity and insect development. ABSTRACT: FK506-binding protein 39kD (FKBP39) localizes in the nucleus and contains multiple functional domains. Structural analysis suggests that FKBP39 might function as a transcriptional factor and control juvenile hormone (JH) activity. Here, we show that FKBP39 expresses at a high level and localizes in the nucleolus of fat body cells during the first two larval stages and early third larval stage. The fkbp39 mutant displays delayed larval-pupal transition and an increased expression of Kr-h1, the main mediator of the JH pathway, at the early third larval stage. Moreover, the fkbp39 mutant has a fertility defect that is independent of JH activity. Interestingly, the expression of rp49, the most widely used reference gene for qRT-PCR in Drosophila, significantly decreased in the fkbp39 mutant, suggesting that FKBP39 might regulate ribosome assembly. Taken together, our data demonstrate the expression pattern and physiological roles of FKBP39 in Drosophila. MDPI 2022-03-28 /pmc/articles/PMC9030728/ /pubmed/35447772 http://dx.doi.org/10.3390/insects13040330 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xinyu
Zhou, Ying
Guan, Jianwen
Cheng, Yang
Lu, Yingying
Wei, Youheng
FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster
title FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster
title_full FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster
title_fullStr FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster
title_full_unstemmed FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster
title_short FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster
title_sort fkbp39 controls the larval stage jh activity and development in drosophila melanogaster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030728/
https://www.ncbi.nlm.nih.gov/pubmed/35447772
http://dx.doi.org/10.3390/insects13040330
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