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PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing

Cyanobacteria are a large group of prokaryotic microalgae that are able to grow photo-autotrophically by utilizing sunlight and by assimilating carbon dioxide to build new biomass. One of the most interesting among many cyanobacteria cell components is the storage biopolymer polyhydroxybutyrate (PHB...

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Autores principales: Meixner, Katharina, Daffert, Christina, Bauer, Lisa, Drosg, Bernhard, Fritz, Ines
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030849/
https://www.ncbi.nlm.nih.gov/pubmed/35447738
http://dx.doi.org/10.3390/bioengineering9040178
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author Meixner, Katharina
Daffert, Christina
Bauer, Lisa
Drosg, Bernhard
Fritz, Ines
author_facet Meixner, Katharina
Daffert, Christina
Bauer, Lisa
Drosg, Bernhard
Fritz, Ines
author_sort Meixner, Katharina
collection PubMed
description Cyanobacteria are a large group of prokaryotic microalgae that are able to grow photo-autotrophically by utilizing sunlight and by assimilating carbon dioxide to build new biomass. One of the most interesting among many cyanobacteria cell components is the storage biopolymer polyhydroxybutyrate (PHB), a member of the group of polyhydroxyalkanoates (PHA). Cyanobacteria occur in almost all habitats, ranging from freshwater to saltwater, freely drifting or adhered to solid surfaces or growing in the porewater of soil, they appear in meltwater of glaciers as well as in hot springs and can handle even high salinities and nutrient imbalances. The broad range of habitat conditions makes them interesting for biotechnological production in facilities located in such climate zones with the expectation of using the best adapted organisms in low-tech bioreactors instead of using “universal” strains, which require high technical effort to adapt the production conditions to the organism‘s need. These were the prerequisites for why and how we searched for locally adapted cyanobacteria in different habitats. Our manuscript provides insight to the sites we sampled, how we isolated and enriched, identified (morphology, 16S rDNA), tested (growth, PHB accumulation) and purified (physical and biochemical purification methods) promising PHB-producing cyanobacteria that can be used as robust production strains. Finally, we provide a guideline about how we managed to find potential production strains and prepared others for basic metabolism studies.
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spelling pubmed-90308492022-04-23 PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing Meixner, Katharina Daffert, Christina Bauer, Lisa Drosg, Bernhard Fritz, Ines Bioengineering (Basel) Communication Cyanobacteria are a large group of prokaryotic microalgae that are able to grow photo-autotrophically by utilizing sunlight and by assimilating carbon dioxide to build new biomass. One of the most interesting among many cyanobacteria cell components is the storage biopolymer polyhydroxybutyrate (PHB), a member of the group of polyhydroxyalkanoates (PHA). Cyanobacteria occur in almost all habitats, ranging from freshwater to saltwater, freely drifting or adhered to solid surfaces or growing in the porewater of soil, they appear in meltwater of glaciers as well as in hot springs and can handle even high salinities and nutrient imbalances. The broad range of habitat conditions makes them interesting for biotechnological production in facilities located in such climate zones with the expectation of using the best adapted organisms in low-tech bioreactors instead of using “universal” strains, which require high technical effort to adapt the production conditions to the organism‘s need. These were the prerequisites for why and how we searched for locally adapted cyanobacteria in different habitats. Our manuscript provides insight to the sites we sampled, how we isolated and enriched, identified (morphology, 16S rDNA), tested (growth, PHB accumulation) and purified (physical and biochemical purification methods) promising PHB-producing cyanobacteria that can be used as robust production strains. Finally, we provide a guideline about how we managed to find potential production strains and prepared others for basic metabolism studies. MDPI 2022-04-18 /pmc/articles/PMC9030849/ /pubmed/35447738 http://dx.doi.org/10.3390/bioengineering9040178 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Meixner, Katharina
Daffert, Christina
Bauer, Lisa
Drosg, Bernhard
Fritz, Ines
PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
title PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
title_full PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
title_fullStr PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
title_full_unstemmed PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
title_short PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
title_sort phb producing cyanobacteria found in the neighborhood—their isolation, purification and performance testing
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030849/
https://www.ncbi.nlm.nih.gov/pubmed/35447738
http://dx.doi.org/10.3390/bioengineering9040178
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