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Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo

Carbon nanotubes (CNTs) are carbon allotropes consisting of one, two, or more concentric rolled graphene layers. These can intrinsically regulate immunity by activating the innate immune system. Mannose receptors (MR), a subgroup of the C-type lectin superfamily, are abundantly expressed on macropha...

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Autores principales: Feng, Haibo, Feng, Yangyang, Lin, Lang, Wu, Daiyan, Liu, Qianqian, Li, Hangyu, Zhang, Xinnan, Li, Sheng, Tang, Feng, Liu, Ziwei, Zhang, Linzi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030879/
https://www.ncbi.nlm.nih.gov/pubmed/35457058
http://dx.doi.org/10.3390/ijms23084239
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author Feng, Haibo
Feng, Yangyang
Lin, Lang
Wu, Daiyan
Liu, Qianqian
Li, Hangyu
Zhang, Xinnan
Li, Sheng
Tang, Feng
Liu, Ziwei
Zhang, Linzi
author_facet Feng, Haibo
Feng, Yangyang
Lin, Lang
Wu, Daiyan
Liu, Qianqian
Li, Hangyu
Zhang, Xinnan
Li, Sheng
Tang, Feng
Liu, Ziwei
Zhang, Linzi
author_sort Feng, Haibo
collection PubMed
description Carbon nanotubes (CNTs) are carbon allotropes consisting of one, two, or more concentric rolled graphene layers. These can intrinsically regulate immunity by activating the innate immune system. Mannose receptors (MR), a subgroup of the C-type lectin superfamily, are abundantly expressed on macrophages and dendritic cells. These play a crucial role in identifying pathogens, presenting antigens, and maintaining internal environmental stability. Utilizing the specific recognition between mannose and antigen-presenting cells (APC) surface mannose receptors, the antigen-carrying capacity of mannose-modified CNTs can be improved. Accordingly, here, we synthesized the mannose-modified carbon nanotubes (M-MWCNT) and evaluated them as an antigen delivery system through a series of in vitro and in vivo experiments. In vitro, M-MWCNT carrying large amounts of OVA were rapidly phagocytized by macrophages and promoted macrophage proliferation to facilitate cytokines (IL-1β, IL-6) secretion. In vivo, in mice, M-MWCNT induced the maturation of dendritic cells and increased the levels of antigen-specific antibodies (IgG, IgG1, IgG2a, IgG2b), and cytokines (IFN-γ, IL-6). Taken together, M-MWCNT could induce both humoral and cellular immune responses and thereby can be utilized as an efficient antigen-targeted delivery system.
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spelling pubmed-90308792022-04-23 Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo Feng, Haibo Feng, Yangyang Lin, Lang Wu, Daiyan Liu, Qianqian Li, Hangyu Zhang, Xinnan Li, Sheng Tang, Feng Liu, Ziwei Zhang, Linzi Int J Mol Sci Article Carbon nanotubes (CNTs) are carbon allotropes consisting of one, two, or more concentric rolled graphene layers. These can intrinsically regulate immunity by activating the innate immune system. Mannose receptors (MR), a subgroup of the C-type lectin superfamily, are abundantly expressed on macrophages and dendritic cells. These play a crucial role in identifying pathogens, presenting antigens, and maintaining internal environmental stability. Utilizing the specific recognition between mannose and antigen-presenting cells (APC) surface mannose receptors, the antigen-carrying capacity of mannose-modified CNTs can be improved. Accordingly, here, we synthesized the mannose-modified carbon nanotubes (M-MWCNT) and evaluated them as an antigen delivery system through a series of in vitro and in vivo experiments. In vitro, M-MWCNT carrying large amounts of OVA were rapidly phagocytized by macrophages and promoted macrophage proliferation to facilitate cytokines (IL-1β, IL-6) secretion. In vivo, in mice, M-MWCNT induced the maturation of dendritic cells and increased the levels of antigen-specific antibodies (IgG, IgG1, IgG2a, IgG2b), and cytokines (IFN-γ, IL-6). Taken together, M-MWCNT could induce both humoral and cellular immune responses and thereby can be utilized as an efficient antigen-targeted delivery system. MDPI 2022-04-11 /pmc/articles/PMC9030879/ /pubmed/35457058 http://dx.doi.org/10.3390/ijms23084239 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Haibo
Feng, Yangyang
Lin, Lang
Wu, Daiyan
Liu, Qianqian
Li, Hangyu
Zhang, Xinnan
Li, Sheng
Tang, Feng
Liu, Ziwei
Zhang, Linzi
Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
title Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
title_full Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
title_fullStr Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
title_full_unstemmed Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
title_short Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
title_sort mannose receptor-mediated carbon nanotubes as an antigen delivery system to enhance immune response both in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030879/
https://www.ncbi.nlm.nih.gov/pubmed/35457058
http://dx.doi.org/10.3390/ijms23084239
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