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Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections
Despite the increasing number of patients suffering from tick-borne encephalitis (TBE), Lyme disease, and their co-infection, the mechanisms of the development of these diseases and their effects on the human body are still unknown. Therefore, the aim of this study was to evaluate the changes in the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031133/ https://www.ncbi.nlm.nih.gov/pubmed/35457192 http://dx.doi.org/10.3390/ijms23084374 |
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author | Gęgotek, Agnieszka Moniuszko-Malinowska, Anna Groth, Monika Pancewicz, Sławomir Czupryna, Piotr Dunaj, Justyna Atalay, Sinemyiz Radziwon, Piotr Skrzydlewska, Elżbieta |
author_facet | Gęgotek, Agnieszka Moniuszko-Malinowska, Anna Groth, Monika Pancewicz, Sławomir Czupryna, Piotr Dunaj, Justyna Atalay, Sinemyiz Radziwon, Piotr Skrzydlewska, Elżbieta |
author_sort | Gęgotek, Agnieszka |
collection | PubMed |
description | Despite the increasing number of patients suffering from tick-borne encephalitis (TBE), Lyme disease, and their co-infection, the mechanisms of the development of these diseases and their effects on the human body are still unknown. Therefore, the aim of this study was to evaluate the changes in the proteomic profile of human plasma induced by the development of TBE and to compare it with changes in TBE patients co-infected with other tick-borne pathogens. The results obtained by proteomic analysis using a nanoLC-Q Exactive HF mass spectrometer showed that the most highly elevated groups of proteins in the plasma of TBE patients with co-infection were involved in the pro-inflammatory response and protein degradation, while the antioxidant proteins and factors responsible for protein biosynthesis were mainly downregulated. These results were accompanied by enhanced GSH- and 4-HNE-protein adducts formation, observed in TBE and co-infected patients at a higher level than in the case of patients with only TBE. In conclusion, the differences in the proteomic profiles between patients with TBE and co-infected patients indicate that these diseases are significantly diverse and, consequently, require different treatment, which is particularly important for further research, including the development of novel diagnostics tools. |
format | Online Article Text |
id | pubmed-9031133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90311332022-04-23 Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections Gęgotek, Agnieszka Moniuszko-Malinowska, Anna Groth, Monika Pancewicz, Sławomir Czupryna, Piotr Dunaj, Justyna Atalay, Sinemyiz Radziwon, Piotr Skrzydlewska, Elżbieta Int J Mol Sci Article Despite the increasing number of patients suffering from tick-borne encephalitis (TBE), Lyme disease, and their co-infection, the mechanisms of the development of these diseases and their effects on the human body are still unknown. Therefore, the aim of this study was to evaluate the changes in the proteomic profile of human plasma induced by the development of TBE and to compare it with changes in TBE patients co-infected with other tick-borne pathogens. The results obtained by proteomic analysis using a nanoLC-Q Exactive HF mass spectrometer showed that the most highly elevated groups of proteins in the plasma of TBE patients with co-infection were involved in the pro-inflammatory response and protein degradation, while the antioxidant proteins and factors responsible for protein biosynthesis were mainly downregulated. These results were accompanied by enhanced GSH- and 4-HNE-protein adducts formation, observed in TBE and co-infected patients at a higher level than in the case of patients with only TBE. In conclusion, the differences in the proteomic profiles between patients with TBE and co-infected patients indicate that these diseases are significantly diverse and, consequently, require different treatment, which is particularly important for further research, including the development of novel diagnostics tools. MDPI 2022-04-15 /pmc/articles/PMC9031133/ /pubmed/35457192 http://dx.doi.org/10.3390/ijms23084374 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gęgotek, Agnieszka Moniuszko-Malinowska, Anna Groth, Monika Pancewicz, Sławomir Czupryna, Piotr Dunaj, Justyna Atalay, Sinemyiz Radziwon, Piotr Skrzydlewska, Elżbieta Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections |
title | Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections |
title_full | Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections |
title_fullStr | Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections |
title_full_unstemmed | Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections |
title_short | Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections |
title_sort | plasma proteomic profile of patients with tick-borne encephalitis and co-infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031133/ https://www.ncbi.nlm.nih.gov/pubmed/35457192 http://dx.doi.org/10.3390/ijms23084374 |
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