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Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA–Protein Complexes Using Mismatch Repair Protein MutS as a Model
Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition. The most common cross-linking methods are based on reactive groups that interact with native or engineered cys...
Autores principales: | Monakhova, Mayya V., Kubareva, Elena A., Kolesnikov, Kirill K., Anashkin, Viktor A., Kosaretskiy, Egor M., Zvereva, Maria I., Romanova, Elena A., Friedhoff, Peter, Oretskaya, Tatiana S., Zatsepin, Timofei S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031232/ https://www.ncbi.nlm.nih.gov/pubmed/35458636 http://dx.doi.org/10.3390/molecules27082438 |
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