Cargando…
Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation
Gastric ulcer is one of the most frequent gastrointestinal disorders, and there is an increasing search for natural products that can heal ulcers and avoid their recurrence. We aimed to evaluate the gastroprotective activity of vanillin, including the investigation of anti-inflammatory activity and...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031284/ https://www.ncbi.nlm.nih.gov/pubmed/35456589 http://dx.doi.org/10.3390/pharmaceutics14040755 |
_version_ | 1784692352625934336 |
---|---|
author | Ciciliato, Murilo Piologo de Souza, Matheus Chiaradia Tarran, Carolina Mendes de Castilho, Ana Laura Tironi Vieira, Ana Júlia Rozza, Ariane Leite |
author_facet | Ciciliato, Murilo Piologo de Souza, Matheus Chiaradia Tarran, Carolina Mendes de Castilho, Ana Laura Tironi Vieira, Ana Júlia Rozza, Ariane Leite |
author_sort | Ciciliato, Murilo Piologo |
collection | PubMed |
description | Gastric ulcer is one of the most frequent gastrointestinal disorders, and there is an increasing search for natural products that can heal ulcers and avoid their recurrence. We aimed to evaluate the gastroprotective activity of vanillin, including the investigation of anti-inflammatory activity and the modulation of gene expression. Wistar rats were orally treated with vehicle, carbenoxolone, or vanillin (25, 50, or 100 mg/kg) and orally received absolute ethanol to develop gastric ulcers. We analyzed the ulcer area, conducted histological analysis, and measured the levels of the inflammatory cytokines TNF-α, IL-6, IL-1β, and IFN-γ, and anti-inflammatory cytokine IL-10 by ELISA. We analyzed mRNA expression for NF-κB, TNF-α, and Il-10. We measured NOx levels using the Griess reaction. Our results showed similar gastroprotection for the three doses. Vanillin increased mucus production and preserved gastric mucosa integrity. The gastroprotective effect was linked to anti-inflammatory activity as a result of decreasing the levels of TNF-α, IL-6, IL-1β, and IFN-γ and increasing IL-10 levels. Vanillin downregulated the mRNA expression of NF-κB and TNF-α, upregulated the mRNA expression of Il-10, and increased NOx levels in the stomach. The gastroprotective activity of vanillin is related to the maintenance of gastric mucus and the local inflammatory response modulation. |
format | Online Article Text |
id | pubmed-9031284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90312842022-04-23 Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation Ciciliato, Murilo Piologo de Souza, Matheus Chiaradia Tarran, Carolina Mendes de Castilho, Ana Laura Tironi Vieira, Ana Júlia Rozza, Ariane Leite Pharmaceutics Article Gastric ulcer is one of the most frequent gastrointestinal disorders, and there is an increasing search for natural products that can heal ulcers and avoid their recurrence. We aimed to evaluate the gastroprotective activity of vanillin, including the investigation of anti-inflammatory activity and the modulation of gene expression. Wistar rats were orally treated with vehicle, carbenoxolone, or vanillin (25, 50, or 100 mg/kg) and orally received absolute ethanol to develop gastric ulcers. We analyzed the ulcer area, conducted histological analysis, and measured the levels of the inflammatory cytokines TNF-α, IL-6, IL-1β, and IFN-γ, and anti-inflammatory cytokine IL-10 by ELISA. We analyzed mRNA expression for NF-κB, TNF-α, and Il-10. We measured NOx levels using the Griess reaction. Our results showed similar gastroprotection for the three doses. Vanillin increased mucus production and preserved gastric mucosa integrity. The gastroprotective effect was linked to anti-inflammatory activity as a result of decreasing the levels of TNF-α, IL-6, IL-1β, and IFN-γ and increasing IL-10 levels. Vanillin downregulated the mRNA expression of NF-κB and TNF-α, upregulated the mRNA expression of Il-10, and increased NOx levels in the stomach. The gastroprotective activity of vanillin is related to the maintenance of gastric mucus and the local inflammatory response modulation. MDPI 2022-03-30 /pmc/articles/PMC9031284/ /pubmed/35456589 http://dx.doi.org/10.3390/pharmaceutics14040755 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ciciliato, Murilo Piologo de Souza, Matheus Chiaradia Tarran, Carolina Mendes de Castilho, Ana Laura Tironi Vieira, Ana Júlia Rozza, Ariane Leite Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation |
title | Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation |
title_full | Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation |
title_fullStr | Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation |
title_full_unstemmed | Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation |
title_short | Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation |
title_sort | anti-inflammatory effect of vanillin protects the stomach against ulcer formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031284/ https://www.ncbi.nlm.nih.gov/pubmed/35456589 http://dx.doi.org/10.3390/pharmaceutics14040755 |
work_keys_str_mv | AT ciciliatomurilopiologo antiinflammatoryeffectofvanillinprotectsthestomachagainstulcerformation AT desouzamatheuschiaradia antiinflammatoryeffectofvanillinprotectsthestomachagainstulcerformation AT tarrancarolinamendes antiinflammatoryeffectofvanillinprotectsthestomachagainstulcerformation AT decastilhoanalauratironi antiinflammatoryeffectofvanillinprotectsthestomachagainstulcerformation AT vieiraanajulia antiinflammatoryeffectofvanillinprotectsthestomachagainstulcerformation AT rozzaarianeleite antiinflammatoryeffectofvanillinprotectsthestomachagainstulcerformation |