Wind Turbine Noise Behaviorally and Physiologically Changes Male Frogs

SIMPLE SUMMARY: We analyzed the behavioral–physiological–immunological interconnected process of Japanese tree frogs (Dryophytes japonicus) during their breeding season when exposed to wind turbine noise. Frogs collected from paddy fields with wind power generators exhibited a faster call rate, higher salivary concentrations of corticosterone, and lower innate immunity. However, frogs exposed to the turbine noise for a short period of time only showed faster call rates. An increase in corticosterone was correlated with an increase in call rate and a decrease in immunity. It seems that the need for energy mobilization due to an increase in the call rate leads to a decrease in innate immunity through an increase in corticosterone. The decrease in immunity due to energy tradeoff or physiological response can change disease epidemiology in the population and create new adaptive patterns within these habitats. ABSTRACT: As the advantages of wind energy as an eco-friendly strategy for power generation continue to be revealed, the number of offshore wind farms also increases worldwide. However, wind turbines can induce behavioral and physiological responses in animals by emitting various types of noises. In this study, we investigated the behavioral, physiological, and immunological responses of male Japanese tree frogs (Dryophytes japonicus) when exposed to wind turbine noise. To determine the effects during the breeding season, frogs were collected from areas with and without wind turbines. Additionally, we exposed the frogs to recorded wind turbine noise at a site without a wind generator for 1 h to 24 h to analyze the short-term effects. Three types of calling patterns (dominant frequency, note duration, and call rate) were analyzed to investigate behavioral responses. Physiological responses were assessed using two steroid hormones assays, namely testosterone and corticosterone detection in the saliva. The immunity of each individual was assessed using a bacterial killing assay. The wind turbine group in the field had a higher call rate and corticosterone levels and lower immunity than the group in the field without turbines present, and all three of these variables were correlated with each other. Conversely, in the noise exposure experiment, a higher call rate was only observed post-exposure compared to pre-exposure. Thus, turbine noise seems to induce decreased immunity in Japanese tree frogs as an increase in energy investment that triggers a behavioral response rather than acting as a sole physiological response that leads to a direct increase in corticosterone. This decreased immunity due to energy tradeoff or physiological response can change the disease epidemiology of the population and create new adaptive patterns in these habitats.