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Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress

Tomato is an important vegetable crop. In the process of tomato production, it will encounter abiotic stress, such as low temperature, drought, and high salt, and biotic stress, such as pathogen infection, which will seriously affect the yield of tomato. Calcium-dependent protein kinase (CDPK) is a...

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Autores principales: Li, Yingying, Zhang, Haixin, Liang, Sibo, Chen, Xiuling, Liu, Jiayin, Zhang, Yao, Wang, Aoxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031491/
https://www.ncbi.nlm.nih.gov/pubmed/35457042
http://dx.doi.org/10.3390/ijms23084227
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author Li, Yingying
Zhang, Haixin
Liang, Sibo
Chen, Xiuling
Liu, Jiayin
Zhang, Yao
Wang, Aoxue
author_facet Li, Yingying
Zhang, Haixin
Liang, Sibo
Chen, Xiuling
Liu, Jiayin
Zhang, Yao
Wang, Aoxue
author_sort Li, Yingying
collection PubMed
description Tomato is an important vegetable crop. In the process of tomato production, it will encounter abiotic stress, such as low temperature, drought, and high salt, and biotic stress, such as pathogen infection, which will seriously affect the yield of tomato. Calcium-dependent protein kinase (CDPK) is a class of major calcium signal receptor which has an important regulatory effect on the perception and decoding of calcium signals. CDPK plays a key role in many aspects of plant growth, such as the elongation of pollen tubes, plant growth, and response to biotic and abiotic stress. While some studies have concentrated on Arabidopsis and pepper, Solanum habrochaites is a wild species relative of cultivated tomato and there is no report on CDPK in Solanum habrochaites to date. Using tomato genomic data, this study identified 33 members of the CDPK gene family. Evolutionary analysis divides family members into four Asian groups, of which the CDPK family members have 11 gene replication pairs. Subcellular location analysis showed that most proteins were predicted to be located in the cytoplasm, and less protein existed on the cell membrane. Not all CDPK family members have a transmembrane domain. Cis regulatory elements relating to light, hormones, and drought stress are overrepresented in the promoter region of the CDPK genes in Solanum habrochaites. The expression levels of each gene under biotic stress and abiotic stress were quantified by qRT-PCR. The results showed that members of the CDPK family in Solanum habrochaites respond to different biotic and abiotic stresses. Among them, the expression of ShCDPK6 and ShCDPK26 genes change significantly. ShCDPK6 and ShCDPK26 genes were silenced using VIGS (virus-induced gene silencing), and the silenced plants illustrated reduced stress resistance to Botrytis cinerea, cold, and drought stress. The results of this study will provide a basis for the in-depth study of the CDPK gene family in Solanum habrochaites, laying the foundation for further analysis of the function of the gene family.
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spelling pubmed-90314912022-04-23 Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress Li, Yingying Zhang, Haixin Liang, Sibo Chen, Xiuling Liu, Jiayin Zhang, Yao Wang, Aoxue Int J Mol Sci Article Tomato is an important vegetable crop. In the process of tomato production, it will encounter abiotic stress, such as low temperature, drought, and high salt, and biotic stress, such as pathogen infection, which will seriously affect the yield of tomato. Calcium-dependent protein kinase (CDPK) is a class of major calcium signal receptor which has an important regulatory effect on the perception and decoding of calcium signals. CDPK plays a key role in many aspects of plant growth, such as the elongation of pollen tubes, plant growth, and response to biotic and abiotic stress. While some studies have concentrated on Arabidopsis and pepper, Solanum habrochaites is a wild species relative of cultivated tomato and there is no report on CDPK in Solanum habrochaites to date. Using tomato genomic data, this study identified 33 members of the CDPK gene family. Evolutionary analysis divides family members into four Asian groups, of which the CDPK family members have 11 gene replication pairs. Subcellular location analysis showed that most proteins were predicted to be located in the cytoplasm, and less protein existed on the cell membrane. Not all CDPK family members have a transmembrane domain. Cis regulatory elements relating to light, hormones, and drought stress are overrepresented in the promoter region of the CDPK genes in Solanum habrochaites. The expression levels of each gene under biotic stress and abiotic stress were quantified by qRT-PCR. The results showed that members of the CDPK family in Solanum habrochaites respond to different biotic and abiotic stresses. Among them, the expression of ShCDPK6 and ShCDPK26 genes change significantly. ShCDPK6 and ShCDPK26 genes were silenced using VIGS (virus-induced gene silencing), and the silenced plants illustrated reduced stress resistance to Botrytis cinerea, cold, and drought stress. The results of this study will provide a basis for the in-depth study of the CDPK gene family in Solanum habrochaites, laying the foundation for further analysis of the function of the gene family. MDPI 2022-04-11 /pmc/articles/PMC9031491/ /pubmed/35457042 http://dx.doi.org/10.3390/ijms23084227 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yingying
Zhang, Haixin
Liang, Sibo
Chen, Xiuling
Liu, Jiayin
Zhang, Yao
Wang, Aoxue
Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
title Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
title_full Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
title_fullStr Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
title_full_unstemmed Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
title_short Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
title_sort identification of cdpk gene family in solanum habrochaites and its function analysis under stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031491/
https://www.ncbi.nlm.nih.gov/pubmed/35457042
http://dx.doi.org/10.3390/ijms23084227
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