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Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes

Fatty acids (FAs) have been shown to exhibit a pro-inflammatory response in various cell types, but astrocytes have been mostly overlooked. FAs, both saturated and unsaturated, have previously been shown to induce pro-inflammatory responses in astrocytes at high concentrations of hundreds of µg/mL....

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Autores principales: Dobri, Ana-Maria, Codrici, Elena, Popescu, Ionela-Daniela, Albulescu, Lucian, Fertig, Emanuel Tudor, Enciu, Ana-Maria, Tanase, Cristiana, Hinescu, Mihail E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031664/
https://www.ncbi.nlm.nih.gov/pubmed/35448516
http://dx.doi.org/10.3390/metabo12040329
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author Dobri, Ana-Maria
Codrici, Elena
Popescu, Ionela-Daniela
Albulescu, Lucian
Fertig, Emanuel Tudor
Enciu, Ana-Maria
Tanase, Cristiana
Hinescu, Mihail E.
author_facet Dobri, Ana-Maria
Codrici, Elena
Popescu, Ionela-Daniela
Albulescu, Lucian
Fertig, Emanuel Tudor
Enciu, Ana-Maria
Tanase, Cristiana
Hinescu, Mihail E.
author_sort Dobri, Ana-Maria
collection PubMed
description Fatty acids (FAs) have been shown to exhibit a pro-inflammatory response in various cell types, but astrocytes have been mostly overlooked. FAs, both saturated and unsaturated, have previously been shown to induce pro-inflammatory responses in astrocytes at high concentrations of hundreds of µg/mL. SSO (Sulfo-N-succinimidyl Oleate sodium), an inhibitor of FA translocase CD36, has been shown to prevent inflammation in the mouse brain by acting on local microglia and infiltrating monocytes. Our hypothesis was that SSO treatment would also impact astrocyte pro-inflammatory response to FA. In order to verify our assumption, we evaluated the expression of pro- and anti-inflammatory cytokines in normal human astrocyte cell culture pre-treated (or not) with SSO, and then exposed to low concentrations of both saturated (palmitic acid) and unsaturated (oleic acid) FAs. As a positive control for astrocyte inflammation, we used fibrillary amyloid. Neither Aβ 1–42 nor FAs induced CD36 protein expression in human astrocytes in cell culture At low concentrations, both types of FAs induced IL-8 protein secretion, and this effect was specifically inhibited by SSO pre-treatment. In conclusion, low concentrations of oleic acid are able to induce an early increase in IL-8 expression in normal human astrocytes, which is specifically downregulated by SSO.
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spelling pubmed-90316642022-04-23 Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes Dobri, Ana-Maria Codrici, Elena Popescu, Ionela-Daniela Albulescu, Lucian Fertig, Emanuel Tudor Enciu, Ana-Maria Tanase, Cristiana Hinescu, Mihail E. Metabolites Article Fatty acids (FAs) have been shown to exhibit a pro-inflammatory response in various cell types, but astrocytes have been mostly overlooked. FAs, both saturated and unsaturated, have previously been shown to induce pro-inflammatory responses in astrocytes at high concentrations of hundreds of µg/mL. SSO (Sulfo-N-succinimidyl Oleate sodium), an inhibitor of FA translocase CD36, has been shown to prevent inflammation in the mouse brain by acting on local microglia and infiltrating monocytes. Our hypothesis was that SSO treatment would also impact astrocyte pro-inflammatory response to FA. In order to verify our assumption, we evaluated the expression of pro- and anti-inflammatory cytokines in normal human astrocyte cell culture pre-treated (or not) with SSO, and then exposed to low concentrations of both saturated (palmitic acid) and unsaturated (oleic acid) FAs. As a positive control for astrocyte inflammation, we used fibrillary amyloid. Neither Aβ 1–42 nor FAs induced CD36 protein expression in human astrocytes in cell culture At low concentrations, both types of FAs induced IL-8 protein secretion, and this effect was specifically inhibited by SSO pre-treatment. In conclusion, low concentrations of oleic acid are able to induce an early increase in IL-8 expression in normal human astrocytes, which is specifically downregulated by SSO. MDPI 2022-04-06 /pmc/articles/PMC9031664/ /pubmed/35448516 http://dx.doi.org/10.3390/metabo12040329 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dobri, Ana-Maria
Codrici, Elena
Popescu, Ionela-Daniela
Albulescu, Lucian
Fertig, Emanuel Tudor
Enciu, Ana-Maria
Tanase, Cristiana
Hinescu, Mihail E.
Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes
title Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes
title_full Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes
title_fullStr Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes
title_full_unstemmed Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes
title_short Low-Concentrations of Fatty Acids Induce an Early Increase in IL-8 Levels in Normal Human Astrocytes
title_sort low-concentrations of fatty acids induce an early increase in il-8 levels in normal human astrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031664/
https://www.ncbi.nlm.nih.gov/pubmed/35448516
http://dx.doi.org/10.3390/metabo12040329
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